<i>In vitro</i>biological evaluation of 100 selected methanol extracts from the traditional medicinal plants of Asia

Li, Chunmei; Wang, Myeong‐Hyeon

doi:10.4162/nrp.2014.8.2.151

Cited by 9 publications

(7 citation statements)

References 25 publications

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“…Medicinal plants contain powerful comfpounds in any of the parts, including the bark, stem, resin, leaf, root, flower, fruit, and seed. Such healing properties of plants cure various health problems [28]. Euphorbia teheranica Boiss.…”

Section: Discussionmentioning

confidence: 99%

“…IC 50 , according to the MTT assay was 850 µg/mL for 24 hours, and 855 µg/mL for 48 hours after treatment. Li et al, reported that whole plant of E. hirta L. showed significant anticancer activity on HT-29 cellline for 24 hours [28]. Also, Patil and Magdum [30], and Sidambaram et al, [31] showed that E. hirta L. had anti-cancer activity on EL-4 mouse lymphoma cell-line and Hep-2 human larynx epithelioma cellline, respectively.…”

Section: Discussionmentioning

confidence: 99%

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Cytotoxic Activity of the Root of Euphorbia Tehranica Ethanolic Extract Against Caco-2 Colorectal Cancer Cell Line

Forouzesh

Sadeghi

Baghaei

et al. 2018

Multidiscip Cancer Investig

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Introduction: Efforts are made to identify the new potential components such as anticancer drugs. Medicinal plants belonging to Euphorbia genus are widely studied with promising effects. The current study aimed at investigating the effect of ethanolic extract of the root of E. tehranica, grown in Iran, on Caco-2 human colorectal cancer cell-line. Methods: The Caco-2 cells were treated with different concentrations of the root of E. tehranica ethanolic extract (25 to 1200 μg/mL) at 24 and 48 hours. Cell growth was evaluated with MTT assay. Anticancer activity of the ethanolic extract was assessed by evaluating the cell viability. Cell viability was determined at the wavelength of 570 nm by MTT method. The IC 50 (half maximal inhibitory concentration) was determined graphically. One-way ANOVA followed by Tukey's post-test; and P values <0.05 were considered statistically significant. Results: The findings showed the significant cytotoxic effect of the root of E. tehranica ethanolic extract against Caco-2 cell-lines (P ≤0.05). Therefore, the viability of Caco-2 cells reduced with the dose and time-dependent manner, when compared with that of the control group. Also, the differences between the IC 50 in 24 and 48 hours were 850 and 855 µg/mL, respectively. Conclusions: It was the first study on the effect of the root of E. tehranica ethanolic extract on human colon cancer cell-lines. The results showed that this extract had the capacity to inhibit proliferation of tumor cells. Therefore, it seems to be a good candidate to defend against cancer cells.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Cytotoxic Activity of the Root of Euphorbia Tehranica Ethanolic Extract Against Caco-2 Colorectal Cancer Cell Line

Forouzesh

Sadeghi

Baghaei

et al. 2018

Multidiscip Cancer Investig

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“…5,38 Previous reports demonstrated the anti-inflammatory effects of the MeOH extracts of G. glabra. 39 To evaluate the anti-inflammatory activities of the 58 compounds, inhibition of LPS-induced NO production in the RAW 264.7 macrophage cell line was assessed. Compounds 4,7,8,15,22,23,26, and 50 at 10 μM inhibited NO secretion by >30% (Table 3).…”

Section: Journal Of Natural Productsmentioning

confidence: 99%

Glycybridins A–K, Bioactive Phenolic Compounds from Glycyrrhiza glabra

Song

et al. 2017

J. Nat. Prod.

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In an attempt to discover bioactive agents from the herbal medicine Glycyrrhiza glabra (widely known as licorice), 11 new phenolic compounds, glycybridins A-K (1-11), along with 47 known phenolics (12-58) were isolated. Their structures were elucidated on the basis of extensive NMR and MS analyses as well as experimental and computed ECD data. According to the clinical therapeutic effects of licorice, enzyme or cell-based bioactivity screenings of 1-58 were conducted. A number of compounds significantly activate Nrf2, inhibit tyrosinase or PTP1B, inhibit LPS-induced NO production and NF-κB transcription, and inhibit the proliferation of human cancer cells (HepG2, SW480, A549, MCF7). Glycybridin D (4) showed moderate cytotoxic activities against the four cancer cell lines, with IC values ranging from 4.6 to 6.6 μM. Further studies indicated that 4 (10 mg/kg, ip) decreased tumor mass by 39.7% on an A549 human lung carcinoma xenograft mice model, but showed little toxicity.

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“…Our previous report (26) has demonstrated considerable anti-inflammatory effect of methanolic extracts from different medicinal plants on LPS-stimulated RAW 264.7 macrophages. In particular, the extract from Glycyrrhiza glabra L. root (EGGR), significantly inhibited nitric oxide production.…”

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confidence: 94%

<i>Glycyrrhiza glabra</i> L. Extract Inhibits LPS-Induced Inflammation in RAW Macrophages

Eom

Jeong

2015

Journal of Nutritional Science and Vitaminology, J Nutr Sci Vit

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Glycyrrhiza glabra has been used in medicine for thousands of years. Our previous study revealed that the methanolic extract of Glycyrrhiza glabra L. (EGGR) exhibits significant nitric oxide (NO) inhibitory effect on lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages among 100 other extracts. Accordingly, the aim of the present study was to investigate the potential anti-inflammatory effect of EGGR. The anti-inflammatory effect of EGGR on LPS-stimulated RAW 264.7 macrophages was measured by MTT assay, NO content analysis, reactive oxygen species (ROS) level analysis, RT-PCR, Western blot analysis, and ELISA assay. Low doses of EGGR were non-toxic to macrophages and imparted protective effect against LPS induced cell death. Incubation of LPS-treated macrophages with 100 μg/mL EGGR led to an increase in cell viability from 66.6 to 99%. Moreover, EGGR led to down regulation of NO (NO2+NO3) and ROS productions in a dose-dependent manner. In particular, 100 μg/mL EGGR led to a reduction in NO2+NO3 level from 336.2 to 24.1 pM/mL, and ROS level from 483.5 to 128.4%. Consistent with the result related to NO production, EGGR suppressed the ability of LPS to induce mRNA and protein expressions of nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) cytokines, tumor necrosis factor-α (TNF-α), interleukin 1β (IL-1β), and IL-6 productions which were analyzed by an ELISA assay. These results provide a comprehensive approach into the anti-inflammatory effect of EGGR on LPS-stimulated macrophages; however, efforts are underway on gaining detailed insight into anti-inflammatory signaling pathways.

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In vitrobiological evaluation of 100 selected methanol extracts from the traditional medicinal plants of Asia

Cited by 9 publications

References 25 publications

Cytotoxic Activity of the Root of Euphorbia Tehranica Ethanolic Extract Against Caco-2 Colorectal Cancer Cell Line

Cytotoxic Activity of the Root of Euphorbia Tehranica Ethanolic Extract Against Caco-2 Colorectal Cancer Cell Line

Glycybridins A–K, Bioactive Phenolic Compounds from Glycyrrhiza glabra

<i>Glycyrrhiza glabra</i> L. Extract Inhibits LPS-Induced Inflammation in RAW Macrophages

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