In vitro biological evaluation of electrospun cellulose acetate fiber mats containing asiaticoside or curcumin

Abstract

Ultra-fine cellulose acetate (CA; M(w) approximately 30,000 Da; degree of acetyl substitution approximately 2.4) fiber mats containing either asiaticoside [from the plant Centella asiatica (L.); either in the form of a crude extract (CACE) or pure substance (PAC)] or curcumin (CM; from the plant Curcuma longa L.) were successfully prepared. The proposed use of these materials is as topical/transdermal patches or wound dressings. Here, the potential for use of these herb-loaded CA fiber mats as wound dressings was evaluated in terms of the stability and the antioxidant activity of the as-loaded herbal substances, the ability to support both the attachment and the proliferation of fibroblasts and the ability of the cultured fibroblasts to synthesize collagen. Normal human dermal fibroblasts (NHDF) were used as the reference fibroblastic cells. The results showed that the as-loaded herbal substances were stable even after the herb-loaded CA fiber mats had been aged either at room temperature or at 40 degrees C for a period of up to 4 months. The inclusion of asiaticoside [either 2% (w/w) CACE or 40% (w/w) PAC] rendered the resulting CA fiber mats their superiority in supporting the attachment, promoting the proliferation, and upregulating the production of collagen of the seeded and/or the cultured NHDF to the corresponding solvent-cast films and the neat CA fiber mats. On the other hand, the presence of CM imparted the antioxidant activity to the resulting CA fiber mats.

Synthesis of Antibacterial Silver–Poly(ɛ-caprolactone)-Methacrylic Acid Graft Copolymer Nanofibers and Their Evaluation as Potential Wound Dressing

“…To disinfect the bacteria attack, numerous active ingredients such as silver [16,17] and iodine complex [18] have been studied. The immobilization of silver to polyvinyl alcohol nanofiber mats showed good antibacterial activity and enhanced a moist environment [16].…”

“…The immobilization of silver to polyvinyl alcohol nanofiber mats showed good antibacterial activity and enhanced a moist environment [16]. Iodine has been incorporated into polyvinylpyrrolidone solution to prepare iodine containing polyvinylpyrrolidone nanofiber mats and the slow release of iodine from the electrospun nanofibers has been studied [19].…”

Electrospinning of Asiaticoside/2-Hydroxypropyl-β-cyclodextrin Inclusion Complex-loaded Cellulose Acetate Fiber Mats: Release Characteristics and Potential for Use as Wound Dressing

“…18,19 The up-regulation of collagen and the increase of tensile strength of guinea pig wounds with topical treatment of varying dosage were observed. 20 Moreover, Suwantong et al 21 observed an increase in synthesized collagen by fibroblasts which had been cultured on a cellulose acetate (CA) fiber mat containing pure AC at a high loading amount (i.e., 40% (wt/wt), based on the weight of CA) in comparison with those cultured on a neat CA fiber.…”

“…We found that the cyclohexene ring moiety of AC is captivated well within the HPβCD cavity (Figure 1(b)). 27 According to Suwantong et al, 21 the efficacy of AC loaded within the electrospun CA fibers in promoting the production of collagen of cultured fibroblasts was only observed at a high concentration of 40% (wt/wt). Here, we aimed to use HPβCD to improve the efficacy of AC at a much lower concentration of AC (i.e., at 10% (wt/wt)).…”

Functional Applications

“…Silk nanofiber mats were electrospun with epidermal growth factor to promote wound healing process (Schneider et al, 2009a), and it has been found the functionalized mat increased the wound closure by 90%. An anti-cancer, anti-oxidant and anti-flammatory substance, curcumin, was loaded into PCL (Merrell et al, 2009) and CA (Suwantong et al, 2010) nanofibers for wound dressing. These dressings were superior in supporting human dermal fibroblast attachment and proliferation and exhibited high rate of wound closure.…”