2019
DOI: 10.1098/rsif.2018.0709
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In vitro cell migration quantification method for scratch assays

Abstract: Motivation:e scratch assay is a standard experimental protocol used to characterize cell migration. It can be used to identify genes that regulate migration and evaluate the e cacy of potential drugs that inhibit cancer invasion. In these experiments, a scratch is made on a cell monolayer and recolonisation of the scratched region is imaged to quantify cell migration rates. A drawback of this methodology is the lack of its reproducibility resulting in irregular cell-free areas with crooked leading edges. Exist… Show more

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Cited by 117 publications
(86 citation statements)
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“…The wound healing and migration assay was adapted from the method described Bobadilla et al [ 16 ]. Briefly, A549 or H1299 cells were seeded into 6-well plates (5 × 10 5 cells/well) and cultured until 90% confluence.…”
Section: Methodsmentioning
confidence: 99%
“…The wound healing and migration assay was adapted from the method described Bobadilla et al [ 16 ]. Briefly, A549 or H1299 cells were seeded into 6-well plates (5 × 10 5 cells/well) and cultured until 90% confluence.…”
Section: Methodsmentioning
confidence: 99%
“…The wound healing and migration assay was adapted from the method described Bobadilla et al [16]. Brie y, A549 or H1299 cells were seeded into 6-well plates (5x10 5 cells/well) and cultured until 90% con uence.…”
Section: Wound Healing and Migration Assaymentioning
confidence: 99%
“…Scratch assays are routinely used to study collective cell behaviour in vitro [1][2][3][4][5]. These experiments are conducted by placing a monolayer of cells on a two-dimensional substrate and creating an artificial wound, or scratch, in the centre of the population (figure 1a-d) [3].…”
Section: Introductionmentioning
confidence: 99%