In vitrocharacterization of a nanostructured fibrin agarose bio-artificial nerve substitute

Abstract

Neural tissue engineering is focused on the design of novel biocompatible substitutes to repair peripheral nerve injuries. In this paper we describe a nanostructured fibrin-agarose bioartificial nerve substitute (NFABNS), based on nanostructured fibrin-agarose hydrogels (FAHs) with human adipose-derived mesenchymal stem cells (HADMSCs). These NFABNSs were mechanically characterized and HADMSCs behaviour was evaluated using histological and ultrastructural techniques. Mechanical characterization showed that the NFABNSs were resistant, flexible and elastic, with a high deformation capability. Histological analyses carried out in vitro during 16 days revealed that the number of HADMSCs decreased over time, with a significant increase after 16 days. HADMSCs formed cell clusters and degraded the surrounding scaffold during this time; additionally, HADMSCs showed active cell proliferation and cytoskeletal remodelling, with a progressive synthesis of extracellular matrix molecules. Finally, this study demonstrated that it is possible to generate biologically active and mechanically stable tissue-like substitutes with specific dimensions, based on the use of HADMSCs, FAHs and a nanostructure technique. However, in vivo analyses are needed to demonstrate their potential usefulness in peripheral nerve repair. Copyright © 2015 John Wiley & Sons, Ltd.

Magnetorheology of alginate ferrogels

“…These anisotropic structures were maintained after cessation of the stress due to the appearance of lateral H-bonds between polymer fibres during drying. Unfortunately, these processes result in a reduction of porosity of the hydrogels and the consequent hindering of cell proliferation, as demonstrated for example for fibrin-based hydrogels [24].…”

Tissue engineering, university and health system. History of an experience

“…Fruto de esta actividad investigadora ha sido la generación de distintos tejidos artificiales como córnea (14) , piel (15,16), mucosa oral (17), nervio periférico (18), paladar (19), cartílago (20), etc. utilizando distintos tipos celulares, biomateriales , procesos de biofabricación (21, 22, 23) y protocolos de control de calidad de los que se ha dado cuenta en publicaciones científicas (24,25,26,27,28,29,30).…”

Type I and type VII agarose characterization for use in tissue engineering

“…Para estudiar el comportamiento celular, se analizaron diferentes procesos celulares mediante técnicas inmunohistoquímicas (13). Se utilizó el antígeno nuclear de células en proliferación (PCNA) para identificar las células proliferantes (20).…”

“…Los grandes poros y la baja rigidez mecánica de los geles a bajas concentraciones de agarosa pueden permitir la migración y proliferación de células (9). Entre sus aplicaciones en Ingeniería Tisular destaca su uso conjugado con fibrina para la obtención de hidrogeles de fibrina-agarosa que permiten la elaboración de tejidos artificiales como piel (10), córnea (11), mucosa oral (12) y nervio (13). Por otro lado, los geles de agarosa suelen también utilizarse, en Ingeniería Tisular, para la reparación del cartílago (14), ya que favorecen el crecimiento y la diferenciación de los condrocitos (15).…”