In vitro characterization of pH-sensitive Bletilla Striata polysaccharide copolymer micelles and enhanced tumour suppression in vivo

Abstract

The above results show that DTX-SA-BSP copolymer micelles have pH sensitivity. SA-BSP copolymers are a promising carrier for delivering hydrophobic anticancer drugs.

Interactions of Self-Assembled Bletilla Striata Polysaccharide Nanoparticles with Bovine Serum Albumin and Biodistribution of Its Docetaxel-Loaded Nanoparticles

“…The docetaxel amount released from the nanoparticles followed an initial rapid release and then a slower constant release, which is beneficial for clinical application. The release pattern is similar with the results for pH 5.0, 6.0, and 7.4 phosphate-buffered saline in our previous publication [22]. We have proved that DTX-loaded BSPs-SA nanoparticles had a pH-sensitive release.…”

“…Namely, the release rate of docetaxel from DTX-loaded BSPs-SA nanoparticles in vitro is accelerated in acidic media compared with that in pH 7.4 phosphate buffer solution (physiological media). Nevertheless, no remarkable differences were observed in the release percentages of Duopafei ® among the three corresponding release media [22]. The differences of docetaxel release rates between Duopafei ® and DTX-loaded BSPs-SA nanoparticles were mainly due to the core–shell structure of BSPs-SA nanoparticles.…”

“…The effects of the drug/carrier on the characterization of DTX-loaded BSPs-SA nanoparticles, the in vitro anticancer activity on HepG2, SW480, MCF-7, and HeLa cells, cellular uptake of DTX-fluorescein isothiocyanate (FITC) labeled-BSPs-SA nanoparticles by a flow cytometry assay, the toxicity of BSPs-SA on human umbilical vein endothelial cells, and in vitro hemolysis assay were also studied [21]. The impacts of pH values (5.0, 6.5, and 7.4) on particle size, zeta potential, in vitro release behavior, the in vitro cytotoxic effect of DTX-loaded BSPs-SA nanoparticles on A549 and MCF-7 cells, quantitative cellular uptake determined by high performance liquid chromatography, determining apoptosis, antitumor effect in vivo in mice bearing 4T1 tumor cells, and the effect of DTX-loaded BSPs-SA nanoparticles on H 2 O 2 -induced hemolysis of red blood cells were investigated [22]. The mice subjected to DTX-loaded BSPs-SA nanoparticles and Duopafei ® displayed an obvious reduction in tumor weight and volume in comparison to that in the model control group ( p < 0.05), respectively.…”

“…The mice subjected to DTX-loaded BSPs-SA nanoparticles and Duopafei ® displayed an obvious reduction in tumor weight and volume in comparison to that in the model control group ( p < 0.05), respectively. Notably, the inhibition capability of DTX-loaded BSPs-SA nanoparticles on tumor cells in vitro and antitumor effects in vivo is superior to that of Duopafei ® [22].…”

A review on the applications of Traditional Chinese medicine polysaccharides in drug delivery systems

“…The hydrophobic core of the SA-BSP enveloped lipophilic DTX, and drug release was ascribed to diffusion and dissolution [ 30 ]. DTX was gradually released from the DTX-SA-BSP micelles, and a steady release rate was maintained for a relatively long time [ 31 ]. Similarly, silymarin (SM) was encapsulated in self-assembled nanoparticles of BSP conjugates modified with SA.…”