In vitro Cholesterol Assimilation by Bifidobacterium animalis subsp. lactis (BPL1) Probiotic Bacteria Under Intestinal Conditions

Abstract

Background: Hypercholesterolemia is one of the principal causes of the development of cardiovascular diseases. Recently, probiotics consumption has been also proposed as a non-pharmacological intervention to control cholesterol concentrations. Objective: To evaluate in vitro assimilation of cholesterol by Bifidobacterium animalis subsp. lactis (BPL1) under simulated intestinal environment in anaerobic conditions and to review and discuss potential physiological mechanisms in this context. Methods: Bacterial viability and cholesterol assimilation was evaluated in both standard MRS and stimulated intestinal fluid (SIF) medium under anaerobic conditions, and in presence or absence of cholesterol. For assimilation assays, cholesterol concentrations in the different suspensions, containing the probiotic or not, was determined by chromatography coupled to mass spectrometry. Results: Results showed that the growth of B. lactis BPL1 under intestinal conditions is favored when cholesterol is present in the culture medium. In addition, a cholesterol assimilation of up to 44.4% under intestinal and anaerobic conditions was observed. Conclusion: Taking into account the revised literature and the experimental results herein presented, administration of functional foodstuffs together with probiotic bacteria such as B. lactis BPL1 could be a potential effective option to decrease hypercholesterolemia, thus preventing the development of cardiovascular diseases. Nevertheless, further studies on mechanisms of effectiveness in animals and clinical trials are still needed.

The Effect of Duck Bile Acid Extract on the Fecal Microbiota, Short Chain Fatty Acids, Fat Digestibility and Blood Biochemical Indices in Cats

“…The mechanism for lowering cholesterol by Bifidobacterium spp. was not very clear until now [24]. The research also found that, after adding…”

“…The mechanism for lowering cholesterol by Bifidobacterium spp. was not very clear until now [24]. The research also found that, after adding BAs, fat digestibility was improved greatly (p < 0.05), which could reduce strain on the liver for cats.…”

“…as the former study showed that Bifidobacterium spp. lowers cholesterol activity [24,25]. The mechanism for lowering cholesterol by Bifidobacterium spp.…”

Evaluation of the Probiotic Properties of Lacticaseibacillus casei 431® Isolated from Food for Special Medical Purposes

“…Bacterial cells remove cholesterol from the medium by adsorption or absorption by incorporating cholesterol into the cell membrane (33). According to the recent literature data, the administration of functional food containing probiotic bacteria was effective in cholesterol assimilation under simulated GIT conditions (34). Nutrient-rich food matrices such as FSMPs might be associated with a higher capacity Please note that this is an unedited version of the manuscript that has been accepted for publication.…”

Відбір Пробіотичних Мікроорганізмів Та Їх Композицій Як Основи Для Лінійки Функціональних Продуктів Харчування З Гіпохолестеринемічними Властивостями

“…3) transformation of cholesterol into coprostanol and its removal from the body together with feces; 4) inhibition of cholesterol synthesis in the liver (Hassan et al, 2019;Bhat et al, 2019;Daliri et al, 2022;Alaqil et al, 2020;O'Morain & Ramji, 2020;Jia et al, 2023;Asan-Ozusaglam & Gunyakti, 2019;Park et al, 2018;Damodharan et al, 2016;Bendali et al, 2017;Singhal et al, 2019;Zhang et al, 2017;Saikia et al, 2018;Bidura et al, 2019;Majeed et al, 2019;Huang et al, 2021;Haldar & Gandhi, 2019;Tom et al, 2021;Palaniyandi et al, 2020;Yusuf et al, 2020;Fernandez-Calderon et al, 2022).…”