<i>In vitro</i> comparison of induction capacity and biomineralization ability of mineral trioxide aggregate and a bioceramic root canal sealer

Güven, Esra Pamukçu; Taşlı, Pakize Neslihan; Yalvaç, Mehmet Emir; Sofiev, N.; Kayahan, Mehmet Baybora; Şahın, Fikrettin

doi:10.1111/iej.12115

Cited by 69 publications

(66 citation statements)

References 47 publications

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“…These results are consistent with previous studies, where mineral deposits were found in cells cultured with ProRoot MTA (15,37,38).…”

Section: Discussionsupporting

confidence: 94%

“…PC, PC 15% and MTA were mixed, placed into Teflon rings (3.5 mm in diameter and 3.5 mm in thickness) and incubated for 24 h at 37°C, 95% humidity and 5% CO 2 . Teflon rings containing the test materials were then placed on insert wells (3.0 µm) (Corning, Lowell, MA), into the 6-well plate and incubated for 15 days (15). The experiment was performed in duplicates.…”

Section: Gene Expression Assaysmentioning

confidence: 99%

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Physico-chemical and Biological Properties of a New Portland Cement-based Root Repair Material

Aminsobhani¹,

MERAJI²,

Khoshdel³

et al. 2017

Eur Endod J

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Objective: To propose bismuth carbonate, a radiopacifying agent, as a new endodontic root repair material that was added to Portland cement (PC) at 2 wt%, 5 wt%, 10 wt% and 15 wt%, and physicochemical and biological properties of each formulation were evaluated in comparison to MTA-Angelus. Methods: Mixed and powder samples were analysed by scanning electron microscopy (SEM) and X-ray power diffraction (XRD), and the semiquantitative constitution of the powder was determined by energy-dispersive spectrometer (EDS). Setting time was evaluated by Vicat needle and radiopacity analysed with digital X-ray. The pH of all tested materials was observed after immersion in water for 3, 24, 48, 72 and 168 h (or 7 days). Solubility and calcium release were measured after immersion in water for 24 h. A multiparametric assay XTT-NR-CVDE was used to evaluate the cytotoxicity of the materials in human periodontal ligament (HPDL) fibroblasts. HPDL fibroblasts were exposed to PC 15% and mineral trioxide aggregate (MTA), and the expression of proinflammatory cytokines (IL1A, IL6, IL8, TNF) and bone formation genes (ALP, COL1 , RUNX2) was evaluated by real-time PCR. Mineralisation of HPDL fibroblasts cocultured with PC, PC 15% and MTA was evaluated with Von Kossa staining. Results: PC-based groups presented more irregular and larger particles than MTA. PC and MTA showed similarities as observed by XRD and EDS. Setting time of PC-based groups was increased with the addition of bismuth carbonate. All tested materials were alkaline, and pH tended to reduce over time. All cements had solubility lower than recommended, with no difference between them (P>0.05) and showed calcium release. PC 15% had similar radiopacity when compared with MTA (P>0.05). Cell viability was higher for the tested materials than the positive control (P<0.001), but there was no difference when they were compared with negative control (P>0.05). Gene expression levels were similar for all tested groups (P>0.05). Analysed cements had positive Von Kossa staining. Conclusion: Overall, the addition of 15% of bismuth carbonate did not result in significant changes to its physicochemical and biological properties when compared with MTA, except for the setting time, and may be considered a potential substitute for MTA. Keywords: Bismuth carbonate, mineral trioxide aggregate, portland cement, root repair material INTRODUCTIONMineral trioxide aggregate (MTA) is a root repair material with Portland cement (PC) and bismuth oxide as major components (1). Several studies have shown that both MTA and PC present similar physicochemical and biological properties (2-5). PC has been proposed as an alternative material to MTA, but its major disadvantage is that it does not achieve the radiopacity level required to be distinguished from the surrounding anatomical structures, thus requiring the addition of a radiopacifying agent prior to its use as an endodontic material (6).Previous studies have shown that bismuth oxide, the radiopacifying agent in ProRoot MTA and MTA-Angelus, decrea...

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“…These results are consistent with previous studies, where mineral deposits were found in cells cultured with ProRoot MTA (15,37,38).…”

Section: Discussionsupporting

confidence: 94%

Section: Gene Expression Assaysmentioning

confidence: 99%

Physico-chemical and Biological Properties of a New Portland Cement-based Root Repair Material

Aminsobhani¹,

MERAJI²,

Khoshdel³

et al. 2017

Eur Endod J

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“…A bioatividade de materiais biocerâmicos endodônticos foi confirmada no Bioagregado 12 , EndoSequence Root Repair Material 12,18 , ProRoo tMTA 12 , iRoot SP 17 . Com relação à adaptação marginal, essa propriedade foi considerada aceitável para materiais biocerâmicos 21,22 , com elevada resistência adesiva nas paredes do canal radicular 24,25 , as quais sofrem pouca influência dos métodos de irrigação 16 . Entretanto, destaca-se que a resistência adesiva desses materiais pode sofrer alteração pela clorexidina 26,27 e remanescentes de hidróxido de cálcio 27 , sendo necessária uma limpeza efetiva do canal radicular.…”

Section: Discussionunclassified

“…A utilização de EDTA a 17%, sozinho ou seguido por 5,25% de NaOCl, CHX a 2% ou solução salina, resultou em resistência adesiva semelhante à do EndoSequence BC Sealer nas paredes dentiná-rias 16 . A resistência adesiva dos cimentos de resina (AH-Plus e Adseal) não é afetada pela solução de irrigação (hipoclorito de sódio ou clorexidina); contudo, a umidade do canal afeta negativamente a resistência de união do AH-Plus.…”

Section: Resistência De União (Push-out)unclassified

Cimentos biocerâmicos em endodontia: revisão de literatura

Lima¹,

Santos²,

Pedrosa³

et al. 2017

Rev. da Fac. de Odontologia, UPF

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Cimentos biocerâmicos vêem se tornando populares na ndodontia como material obturador do sistema de canais radicular devido às suas várias vantagens. Objetivo: Revisar a literatura atualmente disponível sobre as propriedades de cimentos biocerâmicos na endodontia. Metodologia: Uma pesquisa bibliográfica exploratória foi realizada utilizando as bases de dados eletrônicas Public Medline (PubMed), Scopus, Embase, e Web of Science empregando o termo de busca “Bioceramic” AND “Endodontic”. Os critérios de inclusão foram: artigos de pesquisas originais e ensaios clínicos publicados em português, espanhol e inglês. Não foram aplicados limites a data de publicação. Resultados: Foram identificados 30 estudos que abordaram propriedades de cimentos biocerâmicos em endodontia publicados em um período de 7 anos (2011-2017). As propriedades e características vantajosas destacadas nos estudos foram: biocompatibilidade, citotoxicidade, bioatividade, capacidade de manchamento dentário, radiopacidade, pH e extravasamento (extravasamento não se enquadra como propriedade), adaptação marginal, resistência de união, resistência a fratura das raízes, capacidade seladora, e propriedades antibacterianas. Conclusão: Os estudos selecionados para esta revisão de literatura mostram que os cimentos endodônticos biocerâmicos apresentam propriedades promissoras para serem utilizados no tratamento de canais radiculares.

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“…Similar results were reported by Ciasca M et al (2012) 16 and Damas BA et al (2011). 17 In a comparative study done by Guven EP et al (2013) 18 both MTA and EndoSequence have been shown to have 'Apatite-forming ability'. In addition, both the sealers show setting expansion that enhances their seal.…”

Section: Discussionmentioning

confidence: 99%

A Comparative Evaluation of Sealing Ability of Endosequence Bc Sealer and Proroot Mta as Root Canal Sealer: An Ex Vivo Study

Sroa¹,

Sidhu²,

Sharma³

2017

jemds

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BACKGROUNDThe most common method of obturation uses semisolid material such as gutta-percha in combination with a root canal sealer. The sealers must preferably stimulate the process of apical and periapical repair along with providing a hermetic seal. MATERIALS AND METHODSA total of 38 single rooted freshly extracted mandibular teeth were selected and divided into two groups with 15 samples each; Group A (obturated with EndoSequence BC sealer) and Group B (obturated with ProRoot MTA ) and 4 samples each were used for positive control and negative control groups. Sealing ability of the two sealers was evaluated using Microbial Leakage test. RESULTSThe results of the study showed that 5 samples leaked in Group A (33.33%) and 6 samples leaked in Group B (40%). The results of this study suggest that the percentage of specimens without leakage was higher for Group A when compared with Group B. Moreover, early leakage was observed in samples with ProRoot MTA than with EndoSequence BC sealer. However, on applying Mann-Whitney test, the difference was found to be statistically insignificant. Hence, it can be inferred that 'EndoSequence BC Sealer' has comparable sealing ability as compared to 'ProRoot MTA'. CONCLUSIONIt may be concluded that 'EndoSequence BC Sealer' being antibacterial, hydrophilic, bioactive, biocompatible, aluminium-free nanoparticulate bioceramic sealer having superior handling characteristics may be recommended for clinical use. However, before any definite conclusion can be drawn, meticulous studies, a larger number of samples and clinical evaluation for longer duration should be encouraged to evaluate the efficacy of these sealers and potential for achieving the hermetic seal. BACKGROUNDEndodontic sealers are necessary to seal the space between the dentinal walls and the obturating core interface. Moreover, sealers fill the voids and irregularities in the root canal, lateral and accessory canals, and spaces between core materials.Recently, challenges in handling properties of sealers have now been met with the new 'EndoSequence bioceramic sealer' composed of zirconium oxide, calcium silicates, calcium phosphate monobasic and calcium hydroxide along with filler and thickening agents. Since the literature is deficient as regard the sealing ability of EndoSequence bioceramic sealer, the purpose of this study is to evaluate and compare the sealing ability of 'EndoSequence BC sealer (Brassler, Savannah, GA)' in comparison with white 'Pro-Root MTA (Dentsply, Tulsa Dental, Johnson City, USA)' when used as a root canal sealer in an ex vivo study.

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In vitro comparison of induction capacity and biomineralization ability of mineral trioxide aggregate and a bioceramic root canal sealer

Abstract: MTA and iRoot SP induced hTGSC differentiation into odontoblast-like cells, but MTA might provide more inductive potential and hard tissue deposition compared with iRoot SP.

Cited by 69 publications

References 47 publications

Physico-chemical and Biological Properties of a New Portland Cement-based Root Repair Material

Physico-chemical and Biological Properties of a New Portland Cement-based Root Repair Material

Cimentos biocerâmicos em endodontia: revisão de literatura

A Comparative Evaluation of Sealing Ability of Endosequence Bc Sealer and Proroot Mta as Root Canal Sealer: An Ex Vivo Study

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