<i>In vitro</i>copper inhibition of the rabbit spermatozoa motility

Roychoudhury, Shubhadeep; Massányi, Peter

doi:10.1080/10934520801893766

Cited by 35 publications

(16 citation statements)

References 17 publications

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“…The obtained semen sample was diluted with extender mixture for rabbit semen containing antibiotics (6 g in 100 mL Millipore water; Minitub, Germany) according to routine methods. [41][42][43] After processing, the samples were stored in the laboratory at room temperature (20 • C) in air-tight test tubes. For in vitro culture, cadmium (1% CdCl2; Sigma Chemicals, St. Louis, MO, USA; 100 mg/10 mL) was added to semen samples in various concentrations and subsequently diluted.…”

Section: Semen Collection and In Vitro Culturementioning

confidence: 99%

Cadmium toxicity at low concentration on rabbit spermatozoa motility, morphology and membrane integrityin vitro

Roychoudhury

Massányi

Bulla

et al. 2010

Journal of Environmental Science and Health, Part A

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In this study the effect of cadmium on various parameters of spermatozoa motility, morphology as well as on the spermatozoa membrane integrity in rabbits was analyzed in vitro, experimental concentrations ranging from 0.62 to 0.98 micro g CdCl(2)/mL. Pooled rabbit (n = 5) semen was cultured in vitro with cadmium and subsequently diluted to various experimental concentrations apart from control which received no cadmium exposure. Using computer assisted semen analysis method (CASA) we detected decrease of total motility with in the higher concentration range at Time 0. However, with increasing time (after 1 and 2 h of culture), cadmium exerted deleterious effect leading to significant motility reduction in comparison to control. A similar trend was exhibited in case of progressive motility, too. Most of the spermatozoa distance and velocity parameters detected no significant change in comparison to control at the beginning of culture (Time 0), although the toxic effect became significant (P < 0.05) with the passage of culture time (Times 1 and 2 h) in all concentrations. Analysis of spermatozoa morphology detected significant (P < 0.05) alterations at higher concentrations. At higher concentrations acrosomal changes, head without flagellum/separated flagellum, broken flagellum and other abnormalities were significantly higher (P < 0.05), while knob-twisted flagellum and small heads differed significantly (P < 0.05) in comparison to control at all concentrations. In regards to flagellum torso, flagellum ball and retention of cytoplasmic drop statistically higher values (P < 0.05) were noted at the maxium experimental concentration only. Annexin analysis for detection of spermatozoa with disordered membranes revealed higher occurrence of positive spermatozoa in cadmium exposed groups. Annexin-positive reactions suggested alterations in anterior part of head (acrosome) and in flagellum (mitochondrial segment) of spermatozoa. This paper underlines that cadmium is highly toxic for rabbit spermatozoa, as visualized by the toxic effects on parameters of spermatozoa motility, morphology and membrane integrity. The toxic effect is more drastic at higher concentrations. This study also indicates that cadmium requires a minimum one hour incubation time to exert its deletorious effects on various parameters of spermatozoa, particularly at low concentrations.

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Section: Semen Collection and In Vitro Culturementioning

confidence: 99%

Cadmium toxicity at low concentration on rabbit spermatozoa motility, morphology and membrane integrityin vitro

Roychoudhury

Massányi

Bulla

et al. 2010

Journal of Environmental Science and Health, Part A

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“…For example, consuming 2 servings/wk of fish in lieu of 2 servings/wk of processed red meats was associated with an ;60% higher total normal count (95% CI: 17.9, 117.4). serum leads to decreased sperm motility and morphology (42,43) because of oxidative damage (44). Clearly, this intriguing association deserves further study.…”

Section: Figurementioning

confidence: 99%

Processed Meat Intake Is Unfavorably and Fish Intake Favorably Associated with Semen Quality Indicators among Men Attending a Fertility Clinic

Afeiche

Gaskins

Williams

et al. 2014

The Journal of Nutrition

101

105

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Emerging literature suggests that menÕs diets may affect spermatogenesis as reflected in semen quality indicators, but literature on the relation between meat intake and semen quality is limited. Our objective was to prospectively examine the relation between meat intake and indicators of semen quality. Men in subfertile couples presenting for evaluation at the Massachusetts General Hospital Fertility Center were invited to participate in an ongoing study of environmental factors and fertility. A total of 155 men completed a validated food-frequency questionnaire and subsequently provided 338 semen samples over an 18-mo period from 2007-2012. We used linear mixed regression models to examine the relation between meat intake and semen quality indicators (total sperm count, sperm concentration, progressive motility, morphology, and semen volume) while adjusting for potential confounders and accounting for within-person variability across repeat semen samples. Among the 155 men (median age: 36.1 y; 83% white, non-Hispanic), processed meat intake was inversely related to sperm morphology. Men in the highest quartile of processed meat intake had, on average, 1.7 percentage units (95% CI: 23.3, 20.04) fewer morphologically normal sperm than men in the lowest quartile of intake (P-trend = 0.02). Fish intake was related to higher sperm count and percentage of morphologically normal sperm. The adjusted mean total sperm count increased from 102 million (95% CI: 80, 131) in the lowest quartile to 168 million (95% CI: 136, 207) sperm in the highest quartile of fish intake (P-trend = 0.005). Similarly, the adjusted mean percentages of morphologically normal sperm for men in increasing quartiles of fish intake were 5.9 (95% CI: 5.0, 6.8), 5.3 (95% CI: 4.4, 6.3), 6.3 (95% CI: 5.2, 7.4), and 7.5 (95% CI: 6.5, 8.5) (P-trend = 0.01). Consuming fish may have a positive impact on sperm counts and morphology, particularly when consumed instead of processed red meats.

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“…Moreover, Cu deficiency in rams adversely affects Sertoli cell function and impaired the spermatogenic process [5]. On the other hand, high Cu concentration in the semen of rabbits adversely affect the motility of the spermatozoa [21]. The exact reason of increased Cu concentration in the testis of rabbits is unknown, but it could be due to high level of ferroxidase (ceruloplasmin) enzyme in the testis.…”

Section: Discussionmentioning

confidence: 99%

Serum and Testicular Trace Element Concentration in Rabbits at Different Ages

Fayed

2009

Biol Trace Elem Res

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Trace elements such as Zinc (Zn), copper (Cu), iron (Fe), and selenium (Se) are essential for male fertility. The fertilizing capacity of most animals reduced with advancing age. The objective of the present work was to determine the serum and testicular levels of Zn, Cu, and Se in young (10 months old) and old (30 months old) rabbits. Blood and testicular samples were obtained from rabbits after their slaughter. All samples were digested by concentrated acids and analyzed for trace elements by flame emission atomic absorption spectrophotometer. The results showed that serum Zn and Cu were lower in old rabbits than young (P < 0.01). Serum Se was higher in old than young rabbits (P < 0.05). Testicular Cu and Se were significantly lower in old than young rabbits (P < 0.01). However, Zn level was higher in old compared to young rabbits (P < 0.01). The serum Zn and Se were higher than the testicular levels (P < 0.01). The testicular Cu in both ages was higher than the serum level (P < 0.01), which suggest a very important role for Cu in the process of spermatogenesis in rabbits.

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In vitrocopper inhibition of the rabbit spermatozoa motility

Cited by 35 publications

References 17 publications

Cadmium toxicity at low concentration on rabbit spermatozoa motility, morphology and membrane integrityin vitro

Cadmium toxicity at low concentration on rabbit spermatozoa motility, morphology and membrane integrityin vitro

Processed Meat Intake Is Unfavorably and Fish Intake Favorably Associated with Semen Quality Indicators among Men Attending a Fertility Clinic

Serum and Testicular Trace Element Concentration in Rabbits at Different Ages

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