<i>In vitro</i>cytogenetic evaluation of the particular combination of flurbiprofen and roxithromycin

Timocin, Taygun; Hüsunet, Mehmet Tahir; Valipour, Ebrahim; Tazehkand, Mostafa Norizadeh; Çelik, Rima; Topaktaş, Mehmet; İla, Hasan Basri

doi:10.1080/01480545.2016.1223097

Cited by 2 publications

(2 citation statements)

References 43 publications

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“…In the SC form, the plasmid migrates more rapidly than OC and LIN forms. These conformations, in order of electrophoretic mobility from slowest to fastest, are OC DNA, LIN DNA, and SC DNA (Timocin et al , 2017; Fernandez et al , 2011; Simandan et al , 1998; Suksomtip & Pongsamart, 2008). DNA cleavage and protection activities of SRT were evaluated on pBR322 plasmid DNA.…”

Section: Methodsmentioning

confidence: 99%

In vitro cytogenotoxic evaluation of sertraline

İstifli

Çelik

Hüsunet

et al. 2018

Interdisciplinary Toxicology

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Sertraline (SRT) is an antidepressant agent used as a neuronal selective serotonin-reuptake inhibitor (SSRI). SRT blocks serotonin reuptake and increases serotonin stimulation of somatodendritic serotonin 1A receptor (5-HT1AR) and terminal autoreceptors in the brain. In the present study, the genotoxic potential of SRT was evaluated using cytokinesis-block micronucleus (CBMN) cytome assay in peripheral blood lymphocytes of healthy human subjects. DNA cleavage-protective effects of SRT were analyzed on plasmid pBR322. In addition, biochemical parameters of total oxidant status (TOS) and total antioxidant status (TAS) in blood plasma were measured to quantitate oxidative stress. Human peripheral blood lymphocytes were exposed to four different concentrations (1.25, 2.5, 3.75 and 5 μg/mL) of SRT for 24- or 48-h treatment periods. In this study, SRT was not found to induce MN formation either in 24- or 48-h treatment periods. In contrast, SRT concentration-dependently decreased the percentage of MN and MNBN (r=–0.979, p<0.01; r=–0.930, p<0.05, respectively) when it was present for the last 48 hr (48-h treatment) of the culture period. SRT neither demonstrated a cleavage activity on plasmid DNA nor conferred DNA protection against H2O2. The application of various concentrations of SRT significantly increased the TOS and oxidative stress index (OSI) in human peripheral blood lymphocytes for both the 24- and 48-h treatment periods. Morover, the increase in TOS was potent as the positive control MMC at both treatment times. However, SRT did not alter the TAS levels in either 24- or 48-h treatment periods when compared to control. In addition, exposing cells to SRT caused significant decreases in the nuclear division index at 1.25, 2.50 and 3.75 μg/mL in the 24-h and at the highest concentration (5 μg/mL) in the 48-h treatment periods. Our results suggest that SRT may have cytotoxic effect via oxidative stress on cultured human peripheral blood lymphocytes.

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Section: Methodsmentioning

confidence: 99%

In vitro cytogenotoxic evaluation of sertraline

İstifli

Çelik

Hüsunet

et al. 2018

Interdisciplinary Toxicology

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“…It is also used as anti‐inflammatory agent to treat chronic diseases such as bronchitis, diffuse panbronchiolitis, lungs disorder, and bronchial asthma . ROX has also been reported to possess few antitumor effects, for example, ROX induces apoptosis of some T‐cell lines .…”

Section: Introductionmentioning

confidence: 99%

Synthesis of ^99mTc‐roxithromycin: A novel diagnostic agent to discriminate between septic and aseptic inflammation

et al. 2018

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Roxithromycin is a second‐generation macrolide antibiotic derived from erythromycin. In the current study, roxithromycin (ROX) was successfully labeled with technetium‐99m for early diagnosis of bacterial infection and discrimination between septic and aseptic inflammation. The highest radiochemical purity of ≥95% was achieved by investigating different labeling parameters such as pH, ligand/reducing agent concentration, temperature, and amount of stabilizing agent. For this purpose, 0.3–0.5 mg ligand, 2–6 μg SnCl2·2H2O as a reducing agent at basic pH (8–10 pH) and 2 mg mannitol used as a stabilizing agent, in the end, 370 MBq 99mTc added into the reaction vials and incubated for a wide range of temperature (−4 to 65°C). The percent radiochemical purity of 99mTc‐roxithromycin was assessed with the help of the radio‐thin‐layer chromatography technique. The characterization studies were carried out using electrophoresis and Radio‐HPLC techniques as well as saline stability and serum stability studies were also performed. Furthermore, biodistribution study was also performed in an inflamed animal model to discriminate between septic (heat‐killed Staphylococcus aureus) and aseptic (turpentine oil) inflammatory lesions. The results were elaborated that 99mTc‐roxithromycin (99mTc‐ROX) was clearly bounded at the septic inflammation site (T/NT ratio of 7.08 ± 1.14) at 30 min postadministration, and maximum accumulation was seen in heart, lungs, liver, stomach, kidneys, and intestine. The results were suggested that 99mTc‐ROX might be used to discriminate between septic and aseptic inflammatory lesions at an early stage.

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In vitrocytogenetic evaluation of the particular combination of flurbiprofen and roxithromycin

Cited by 2 publications

References 43 publications

In vitro cytogenotoxic evaluation of sertraline

In vitro cytogenotoxic evaluation of sertraline

Synthesis of ^99mTc‐roxithromycin: A novel diagnostic agent to discriminate between septic and aseptic inflammation

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In vitrocytogenetic evaluation of the particular combination of flurbiprofen and roxithromycin

Cited by 2 publications

References 43 publications

In vitro cytogenotoxic evaluation of sertraline

In vitro cytogenotoxic evaluation of sertraline

Synthesis of 99mTc‐roxithromycin: A novel diagnostic agent to discriminate between septic and aseptic inflammation

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Synthesis of ^99mTc‐roxithromycin: A novel diagnostic agent to discriminate between septic and aseptic inflammation