In VitroCytotoxic, Antioxidant, and Antimicrobial Activities ofMesua beccariana(Baill.) Kosterm.,Mesua ferreaLinn., andMesua congestifloraExtracts

Abstract

The in vitro cytotoxicity tests on the extracts of Mesua beccariana, M. ferrea, and M. congestiflora against Raji, SNU-1, HeLa, LS-174T, NCI-H23, SK-MEL-28, Hep-G2, IMR-32, and K562 were achieved using MTT assay. The methanol extracts of Mesua beccariana showed its potency towards the proliferation of B-lymphoma cell (Raji). In addition, only the nonpolar to semipolar extracts (hexane to ethyl acetate) of the three Mesua species indicated cytotoxic effects on the tested panel of human cancer cell lines. Antioxidant assays were evaluated using DPPH scavenging radical assay and Folin-Ciocalteu method. The methanol extracts of M. beccariana and M. ferrea showed high antioxidant activities with low EC50 values of 12.70 and 9.77 μg/mL, respectively, which are comparable to that of ascorbic acid (EC50 = 5.62 μg/mL). Antibacterial tests were carried out using four Gram positive and four Gram negative bacteria on Mesua beccariana extracts. All the extracts showed negative results in the inhibition of Gram negative bacteria. Nevertheless, methanol extracts showed some activities against Gram positive bacteria which are Bacillus cereus, methicillin-sensitive Staphylococcus aureus (MSSA), and methicillin-resistant Staphylococcus aureus (MRSA), while the hexane extract also contributed some activities towards Bacillus cereus.

Molecular mechanisms responsible for programmed cell death-inducing attributes of terpenes from Mesua ferrea stem bark towards human colorectal carcinoma HCT 116 cells

“…Subsequently, using bioactivity guided approach from the SB crude soxhlet extracts, non-polar (n-Hex) extract and its terpenes rich-fraction (F-3) were selected for additional studies. Observations of current study are consistent with other studies where n-Hex extract from root bark of M. ferrea was shown to be more cytotoxic towards a panel of human cancer cell lines compared with other solvent extracts (Teh et al, 2013). Noteworthy, the current study reports for the first time the in vitro cytotoxic activity of M. ferrea stem bark against HCT 116, PC-3, PANC-1, Capan-1, MIA PaCa-2 and MKN-74 cell lines respectively whereas other studies reported the anticancer activities of blossoms, leaves, flowers, seeds and root bark extracts of M. ferrea (Adewale et al, 2012;Mahavorasirikul et al, 2010;Sukanya and Samart, 2012;Teh et al, 2011Teh et al, , 2013.…”

“…Mesua ferrea (Penaga lilin) is an ornamental plant of family guttiferae which is extensively studied for its anticancer activities in Asian countries including Malaysia. In vitro anticancer activities of M. ferrea against a variety of cancer cell lines i.e., MCF-7, KB, NCI-H187, NCI-H23, Raji, SNU-1, K562, LS-174T, HeLa, SK-MEL-28, IMR-32, Hep-G2, HT-29 and LIM1215 have already been reported (Mahavorasirikul et al, 2010;Teh et al, 2011Teh et al, , 2013Sukanya and Samart, 2012;Asif et al, 2016b). However, to best of our knowledge, there is no detailed study available that reports the molecular mechanisms responsible for the anticancer effects of M. ferrea stem bark extract against HCT 116 (human colorectal carcinoma) cell line.…”

Recovery and Utilization of Palm Oil Mill Effluent Source as Value-Added Food Products

“…The total phenolic content of extracts were measured using Folin-Ciocalteu method with some modifications as described by Teh et al 22 . Gallic acid was used as the standard in the experiment.…”

“…Scavenging activity of extracts were assessed using DPPH free radical assay 22 . Ascorbic acid was used as the standard in the experiment.…”

Isoledene from Mesua ferrea oleo-gum resin induces apoptosis in HCT 116 cells through ROS-mediated modulation of multiple proteins in the apoptotic pathways: A mechanistic study

“…In addition, in vitro anticancer activities of different parts of M. ferrea towards a variety of cancer cell lines i.e., MCF-7, KB, NCI-H187, NCI-H23, Raji, SNU-1, K562, LS-174T, HeLa, SK-MEL-28, IMR-32 and Hep-G2 have also been reported (Keawsaard et al, 2015;Mahavorasirikul et al, 2010;Teh et al, 2011;Teh et al, 2013).…”