<i>In vitro</i>
            cytotoxic capacity against tumor cell lines and antioxidant activity of acidic polysaccharides isolated from the Andean Patagonian fungus
            <i>Phylloporia boldo</i>

Albornoz, Verónica; Casas-Arrojo, Virginia; Figueroa, Fabián; Riquelme, Cristian; Hernández, Vı́ctor; Rajchenberg, Mario; Smith, Carlos T.; Campos, Víctor; Abdala-Díaz, Roberto; Becerra, José; Pérez, Claudia A.; Cabrera‐Pardo, Jaime R.

doi:10.1080/14786419.2022.2158331

Natural Product Research

2022

DOI: 10.1080/14786419.2022.2158331

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In vitro cytotoxic capacity against tumor cell lines and antioxidant activity of acidic polysaccharides isolated from the Andean Patagonian fungus Phylloporia boldo

Verónica Albornoz

Virginia Casas-Arrojo

Fabián Figueroa

et al.

Abstract: Fungal polysaccharides possess a broad biological activity, including cytotoxic and antioxidant activities. This work aimed to evaluate the cytotoxic and antioxidant activity of the acidic polysaccharides of Phylloporia boldo strain (named PBAP40). Cytotoxic activity of polysaccharide was evaluated determining the viability of three tumor cell lines by MTT assay. The effect of acidic polysaccharide on the cell cycle of HL-60 cell line was evaluated by flow cytometry, and the antioxidant activity was determined… Show more

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2024

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Cited by 4 publications

References 21 publications

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Metabolomics analysis of rice fermented by medicinal fungi providing insights into the preparation of functional food

Luo,

Jiang,

Liu

et al. 2024

Food Chemistry

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Metabolomics analysis of rice fermented by medicinal fungi providing insights into the preparation of functional food

Luo,

Jiang,

Liu

et al. 2024

Food Chemistry

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In vitro anti-tumoral and immunomodulatory effects of acidic polysaccharides isolated from the fungus Gloeosoma mirabile

Torres,

Figueroa,

Casas Arrojo

et al. 2024

Natural Product Research

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Polysaccharides from wood-rooting fungi have attracted attention due to their broad pharmacological properties. Herein, we report the antitumor and immunomodulatory activities of acid polysaccharides isolated from fungi Gloeosoma mirabile. The polysaccharide extracts displayed significant antiproliferative activity against cancer cell lines (MCF-7, HCT-116, U-937) in a dose-dependent manner and induction of IL-6 in macrophage RAW 264.7. Furthermore, flow cytometry analysis showed that high polysaccharide concentrations induced apoptosis by 83% in HL-60 cells. Based on gas chromatography-mass spectrometry (GC-MS) and Fourier transform infrared (FT-IR) spectroscopy studies, acidic polysaccharides from G. mirabile were mainly composed of arabinose, α-D-galactopyranose and methyl β-D-galactopyranoside. 2 Experimental Materials and Reagents The reagents were obtained from Merck (Darmstadt, Germany). Culture medium, Dulbecco's Modified Eagle Medium-DMEM (Biowest, REF: L0103-500) and RPMI-1640 (Biowest, REF: L0500-500) were purchased from Biowest. Cell culture medium components such as: fetal bovine serum (FBS) (Biowest, REF: S1810-500), penicillinstreptomycin solution 100X (Biowest, REF: L0022-020) and amphotericin B (Biowest, REF: L0009-020) were also purchased from Biowest. Phosphate buffered saline (PBS) were purchased from Invitrogen Corporation (Burlington, ON, USA). Macrophage cell line RAW 264.7 and tumoral cells was purchased from ATTC. The Interleukin-6 was purchased from Invitrogen. All other solvents and chemicals were of analytical grade. Derivatization reagent (BSTFA) was obtained from Sigma Aldrich Fluka (Fluka, U.S.A.) and MTT [(3-(4,5dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide was obtained from Sigma Aldrich (Sigma, MO, USA). Distilled water was used in all the experiments. Determination of Gloeosoma mirabile The strain of Gloesoma mirabile (Berk. & M.A.Curtis) Rajchenb., Pildain & C.Riquelme (Stereaceae, Russulales, Agaricomycetes) was obtained from basidiomes collected from Brazil,

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Investigation on the reversal effect of closantel on colistin resistance in MCR-1 positive Escherichia coli based on dose–response relationship

Cui,

Liu,

Wang

et al. 2024

Journal of Antimicrobial Chemotherapy

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Background The lack of research on the dose–response relationship of adjuvants in reversing colistin resistance will lead to a lack of scientific theoretical basis for determining the dosage of adjuvants in clinical combination therapy plans or their compound formulations. Objectives This study investigates the dose–response relationship of the deworming drug closantel (CST) on the reversal of colistin resistance in mcr-1-positive Escherichia coli (E. coli). Methods Firstly, the reversal effect of different concentrations of CST on colistin resistance in mcr-1-positive E. coli was analysed using broth microdilution method, checkerboard method and time-killing curves. Then, the inhibitory effect of CST on the development of colistin resistance, as well as the haemolytic and cytotoxic properties of CST, was analysed. Finally, the in vivo efficacy of the combination of CST and colistin was evaluated. Results Both the checkerboard assays and the time-killing curves indicate that there is a special dose–response relationship between CST and its reversal effect on colistin resistance, which is not concentration-dependent. High reversal efficiency can be achieved within a low concentration range. However, as the CST concentration increases, the ability to reverse colistin resistance remains unchanged or decreases, which resulted in a gradual decrease in reversal efficiency. Additionally, CST can inhibit the development of colistin resistance and reduce the cytotoxicity of colistin. Importantly, in a mouse model of E. coli infection, the combination of CST and colistin showed a significant therapeutic effect. Conclusions This study indicates a special dose–response relationship between CST and its reversal effect on colistin resistance, which was not concentration-dependent.

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In vitro cytotoxic capacity against tumor cell lines and antioxidant activity of acidic polysaccharides isolated from the Andean Patagonian fungus Phylloporia boldo

Cited by 4 publications

References 21 publications

Metabolomics analysis of rice fermented by medicinal fungi providing insights into the preparation of functional food

Metabolomics analysis of rice fermented by medicinal fungi providing insights into the preparation of functional food

In vitro anti-tumoral and immunomodulatory effects of acidic polysaccharides isolated from the fungus Gloeosoma mirabile

Investigation on the reversal effect of closantel on colistin resistance in MCR-1 positive Escherichia coli based on dose–response relationship

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