In Vitro Cytotoxic Study of Euphorbia grantii Oliv. Aerial Parts against MCF-7 and MCF-7ADR Breast Cancer Cell Lines: A Bioactivity-Guided Isolation

Radi, Marco; El-Shiekh, Riham A.; El-Halawany, Ali M.; Al‐Abd, Ahmed M.; Abdel‐Sattar, Essam

doi:10.1021/acsomega.3c02091

Cited by 7 publications

(2 citation statements)

References 33 publications

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“…This compound avoids anti-liver metastasis. Factor L1, Euphylbenzoate, and Glutinol induced cell death through apoptosis [ 39 , 51 , 73 ].…”

Section: Discussionmentioning

confidence: 99%

Anti-Inflammatory and Cytotoxic Compounds Isolated from Plants of Euphorbia Genus

Rojas-Jiménez,

Valladares-Cisneros,

Salinas-Sánchez

et al. 2024

Molecules

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Euphorbia is a large genus of the Euphorbiaceae family. Around 250 species of the Euphorbia genus have been studied chemically and pharmacologically; different compounds have been isolated from these species, especially diterpenes and triterpenes. Several reports show that several species have anti-inflammatory activity, which can be attributed to the presence of diterpenes, such as abietanes, ingenanes, and lathyranes. In addition, it was found that some diterpenes isolated from different Euphorbia species have anti-cancer activity. In this review, we included compounds isolated from species of the Euphorbia genus with anti-inflammatory or cytotoxic effects published from 2018 to September 2023. The databases used for this review were Science Direct, Scopus, PubMed, Springer, and Google Scholar, using the keywords Euphorbia with anti-inflammatory or cytotoxic activity. In this review, 68 studies were collected and analyzed regarding the anti-inflammatory and anti-cancer activities of 264 compounds obtained from 36 species of the Euphorbia genus. The compounds included in this review are terpenes (95%), of which 68% are diterpenes, especially of the types ingenanes, abietanes, and triterpenes (approximately 15%).

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“…This compound avoids anti-liver metastasis. Factor L1, Euphylbenzoate, and Glutinol induced cell death through apoptosis [ 39 , 51 , 73 ].…”

Section: Discussionmentioning

confidence: 99%

Anti-Inflammatory and Cytotoxic Compounds Isolated from Plants of Euphorbia Genus

Rojas-Jiménez,

Valladares-Cisneros,

Salinas-Sánchez

et al. 2024

Molecules

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“…It is to be noted that the discovery of most of natural products has been driven by bioactivity-guided fractionation of chemical extracts [27]. Currently, although new strategies have been introduced [28,29], this approach remains effective [30][31][32][33][34]. To this end, aqueous extracts obtained under different pH conditions from both untreated and defatted seeds were investigated for their antibiofilm effect against Staphylococcus epidermidis.…”

Section: Introductionmentioning

confidence: 99%

Aqueous Extracts from Hemp Seeds as a New Weapon against Staphylococcus epidermidis Biofilms

Casillo,

D’Angelo,

Imbimbo

et al. 2023

IJMS

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This study investigated the antibiofilm activity of water-soluble extracts obtained under different pH conditions from Cannabis sativa seeds and from previously defatted seeds. The chemical composition of the extracts, determined through GC-MS and NMR, revealed complex mixtures of fatty acids, monosaccharides, amino acids and glycerol in ratios depending on extraction pH. In particular, the extract obtained at pH 7 from defatted seeds (Ex7d) contained a larger variety of sugars compared to the others. Saturated and unsaturated fatty acids were found in all of the analysed extracts, but linoleic acid (C18:2) was detected only in the extracts obtained at pH 7 and pH 10. The extracts did not show cytotoxicity to HaCaT cells and significantly inhibited the formation of Staphylococcus epidermidis biofilms. The exception was the extract obtained at pH 10, which appeared to be less active. Ex7d showed the highest antibiofilm activity, i.e., around 90%. Ex7d was further fractionated by HPLC, and the antibiofilm activity of all fractions was evaluated. The 2D-NMR analysis highlighted that the most active fraction was largely composed of glycerolipids. This evidence suggested that these molecules are probably responsible for the observed antibiofilm effect but does not exclude a possible synergistic contribution by the other components.

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LC-QToF chemical profiling of Euphorbia grantii Oliv. and its potential to inhibit LPS-induced lung inflammation in rats via the NF-κB, CY450P2E1, and P38 MAPK14 pathways

Radi,

El-Shiekh,

Hegab

et al. 2023

Inflammopharmacol

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Acute lung injury (ALI) is a life-threatening syndrome that causes high morbidity and mortality worldwide. The aerial parts of Euphorbia grantii Oliv. were extracted with methanol to give a total methanolic extract (TME), which was further fractionated into dichloromethane (DCMF) and the remaining mother liquor (MLF) fractions. Biological guided anti-inflammatory assays in vitro revealed that the DCMF showed the highest activity (IC50 6.9 ± 0.2 μg/mL and 0.29 ± 0.01 μg/mL) compared to. celecoxib (IC50 of 88.0 ± 1 μg/mL and 0.30 ± 0.01 μg/mL) on COX-1 and COX-2, respectively. Additionally, anti-LOX activity was IC50 = 24.0 ± 2.5 μg/mL vs. zileuton with IC50 of 40.0 ± 0.5 μg/mL. LC-DAD-QToF analysis of TME and the active DCMF resulted in the tentative identification and characterization of 56 phytochemical compounds, where the diterpenes were the dominated metabolites. An LPS-induced inflammatory model of ALI (10 mg/kg i.p) was used to assess the anti-inflammatory potential of DCMF in vivo at dose of 200 mg/kg and 300 mg/kg compared to dexamethasone (5 mg/kg i.p). Our treatments significantly reduced the pro-inflammatory cytokines (TNF-α, IL-1, IL-6, and MPO), increased the activity of antioxidant enzymes (SOD, CAT, and GSH), decreased the activity of oxidative stress enzyme (MDA), and reduced the expression of inflammatory genes (p38.MAPK14 and CY450P2E1). The western blotting of NF-κB p65 in lung tissues was inhibited after orally administration of the DCMF. Histopathological study of the lung tissues, scoring, and immunohistochemistry of transforming growth factor-beta 1 (TGF-β1) were also assessed. In both dose regimens, DCMF of E. grantii prevented further lung damage and reduced the side effects of LPS on acute lung tissue injury.

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In Vitro Cytotoxic Study of Euphorbia grantii Oliv. Aerial Parts against MCF-7 and MCF-7^ADR Breast Cancer Cell Lines: A Bioactivity-Guided Isolation

Cited by 7 publications

References 33 publications

Anti-Inflammatory and Cytotoxic Compounds Isolated from Plants of Euphorbia Genus

Anti-Inflammatory and Cytotoxic Compounds Isolated from Plants of Euphorbia Genus

Aqueous Extracts from Hemp Seeds as a New Weapon against Staphylococcus epidermidis Biofilms

LC-QToF chemical profiling of Euphorbia grantii Oliv. and its potential to inhibit LPS-induced lung inflammation in rats via the NF-κB, CY450P2E1, and P38 MAPK14 pathways

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