In vitrocytotoxicity effects of date palm (Phoenix dactyliferaL.) pollen on neonate mouse spermatogonial stem cells

Abstract

There is a fast growing tendency in the use of herbal remedies in developing countries. One of the traditional medicines used for male infertility treatment is date palm (Phoenix dactylifera) pollen (DPP). Isolated spermatogonial stem cells and sertoli cells using enzymatic digestion were grown in Dulbecco's modified Eagle's medium supplemented with 4% foetal bovine serum in the absence or presence of 0.06, 0.25 and 0.62 mg/mL concentrations of aqueous extract of DPP for 2 weeks. The assessment of mean number of the whole cells and the living cells showed that there were no significant differences between the mean viability percentage and proliferation rate between control and experimental groups (P>0.05). As there are no cytotoxicity effects of DPP in our cultural system, this system can be utilised for the enrichment or differentiation of these cells in clinical applications, cell replacement therapy, tissue regeneration and tissue engineering applications.

Graminex Pollen: Phenolic Pattern, Colorimetric Analysis and Protective Effects in Immortalized Prostate Cells (PC3) and Rat Prostate Challenged with LPS

“…The inhibitory effects on cell viability could be related to the capability of steroid fraction of pollen to induce PC3 cell apoptosis [ 29 ]. On the other hand, the aqueous pollen extract did not modify the viability of intestinal Caco-2, spermatogonial stem cells and sertoli cell lines [ 30 , 31 ]. In PC3 cells, we also observed a protective effect induced by the aqueous pollen extract (10–100 μg/mL) supplementation, which was able to inhibit hydrogen peroxide-induced ROS production ( Figure 2 ).…”

Efficacy of Ficus carica leaf extract on morphological and molecular behavior of mice germ stem cells

“…One of the most significant current attention of in vitro SSCs enrichment is the use of antioxidants to break the oxidative chain reaction (Naskar et al, 2010). In recent years, there have been increasing amounts of literature on herbal medicines as the preferred choice for infertility treatment due to the presence of antioxidants in plants (Safarnavadeh and Rastegarpanah, 2011;Mahaldashtian et al, 2015Mahaldashtian et al, , 2016Naghdi et al, 2016).…”