In vitro degradation and protein release of semi‐IPN hydrogels consisted of poly(acrylic acid‐acrylamide‐methacrylate) and amylose

Abstract

ABSTRACT:The enzymatic degradation mechanism of semi-interpenetrating network (semi-IPN) hydrogel of poly (acrylic acid-acrylamide-methacrylate) crosslinked by azocompound and amylose in vitro was investigated in the presence of Fungamyl 800L (a-amylase) and rat cecum content (cecum bacteria). The degradation mechanism involves degradable competition, i.e., reduction of azo crosslinkage is dominant in the earlier period of degradation. Subsequently, the degradation of gels is continued by combination of reduction of azo crosslinkage and hydrolysis of amylose. The cumulative release ratios of Bovine serum albumin (BSA, as a model drug) loaded semi-IPN gels are 25% in pH 2.2 buffer solutions and 74% in pH 7.4 buffer solutions within 48 h. Moreover, the release behavior of BSA from the semi-IPN gels indicates that it follows Fickian diffusion mechanism in pH 2.2 media and non-Fickian diffusion and polymer chains relaxation mechanism in pH 7.4 media. The results indicate that the release of BSA from the semi-IPN gels was controlled via a combined mechanism of pH dependent swelling and specificity to enzymatic degradation.

Synthesis, characterization and evaluation of semi‐IPN hydrogels consisted of poly(methacrylic acid) and guar gum for colon‐specific drug delivery

“…Similar results were also found in our previous work. 11 The degradation of the hydrogels was further confirmed by SEM micrographs of the gels before and after enzymatic degradation (Fig. 5).…”

“…In vitro release of 5-ASA of the hydrogels was carried out by a method from the literature. 11 Briefly, dehydrated 5-ASA loaded hydrogels were immersed at 378C in vials containing 20 ml of phosphate buffer solution with or without addition of rat cecum content. For those added rat cecum content, the above solution was bubbled with nitrogen for 5 min to obtain anaerobic conditions, and the vials were closed, tightly sealed, and incubated in a thermostatic rotary shaker (HQ45Z, Chinese Academy of Sciences Instrument Corp. Ltd., Wuhan, China) at shaking speed of 50 rpm.…”

Removal of Cationic Dyes from Aqueous Solution by Hydrophobically Modified Poly(acrylic Acid) Hydrogels

“…These polymeric hydrogels often have porous structure networks and allow solute diffusion through the hydrogel structure [9][10][11][12][13] . Moreover, these polymeric hydrogels can absorb and trap ionic dyes because that they possess ionic functional groups.…”

“…The hydrogels were prepared through aqueous free radical polymerization of AA with n-alkyl methacrylate (MMA, EMA and BMA), according to the method described earlier [9] . The feed composition for the preparation of hydrogels is listed in Table 2.…”

The concept of self-assembling and the interactions involved

“…Starch copolymerized and cross-linked simultaneously with vinyl monomers generated good superabsorbence and also a matrix for drug delivery systems (Li et al, 2007;Parvathy and Jyothi, 2012).…”