<i>In vitro</i> development of secondary follicles from pre-pubertal and adult goats cultured in two-dimensional or three-dimensional systems

Silva, Gerlane Modesto da; Rossetto, R.; Chaves, Roberta Nogueira; Duarte, A.B.G.; Araújo, Valdevane Rocha; Feltrin, Cristiano; Bernuci, Marcelo Picinin; Anselmo-Franci, Janete Aparecida; Xu, Min; Woodruff, Teresa K.; Campello, C.C.; Figueiredo, J.R.

doi:10.1017/s0967199414000070

Cited by 52 publications

(36 citation statements)

References 28 publications

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“…Thus, there are many complexities when growing follicles in culture. That said, we have had success with the mouse, goat, dog, bovine, baboon and rhesus monkey (Xu et al 2006a, Xu et al 2009a, Xu et al 2009b, Xu et al 2010, Songsasen et al 2011, Xu et al 2011a, Silva et al 2014) and we, and others continue to develop in vivo and in vitro techniques to advance the field (De Vos et al 2014). We believe the discoveries described in the present report will further refine the eIVFG system advancing the clinical application of human follicle culture for women who wish to preserve their fertility in the face of diseases or treatments that may compromise their reproductive health.…”

Section: Discussionmentioning

confidence: 99%

Size-specific follicle selection improves mouse oocyte reproductive outcomes

Xiao¹,

Duncan

Bai³

et al. 2015

Reproduction

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Encapsulated in vitro follicle growth (eIVFG) has great potential to provide an additional fertility preservation option for young women and girls with cancer or other reproductive health threatening diseases. Currently, follicles are cultured for a defined period of time and analyzed as a cohort. However, follicle growth is not synchronous, and culturing follicles for insufficient or excessive times can result in compromised gamete quality. Our objective is to determine whether the selection of follicles based on size, rather than absolute culture time, better predict follicle maturity and oocyte quality. Multilayer secondary mouse follicles were isolated and encapsulated in 0.25% alginate. Follicles were cultured individually either for defined time periods or up to specific follicle diameter ranges, at which point several reproductive endpoints were analyzed. The metaphase II (MII) percentage after oocyte maturation on day 6 was the highest (85%) when follicles were cultured for specific days. However, if follicles were cultured to a terminal diameter of 300–350 μm irrespective of absolute time in culture, 93% of the oocytes reached MII. More than 90% of MII oocytes matured from follicles with diameters of 300–350 μm showed normal spindle morphology and chromosome alignment, 85% of oocytes showed 2 pronuclei after in vitro fertilization (IVF), 81% developed into the 2-cell embryo stage, and 38% developed to the blastocyst stage, all significantly higher than the percentages in the other follicle size groups. Our study demonstrates that size-specific follicle selection can be used as a non-invasive marker to identify high quality oocytes and improve reproductive outcomes during eIVFG.

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Section: Discussionmentioning

confidence: 99%

Size-specific follicle selection improves mouse oocyte reproductive outcomes

Xiao¹,

Duncan

Bai³

et al. 2015

Reproduction

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“…This system has provided encouraging results for advancing follicle development in the human [8–10], rhesus monkey [11], baboon [12], goat [13], and dog [14, 15], although with limited or no success at producing fertilizable oocytes. The lack of direct application from achievements in the mouse to other species likely is due to physical, anatomical and physiological differences [15, 16].…”

Section: Introductionmentioning

confidence: 99%

Short-term hypertonic exposure enhances in vitro follicle growth and meiotic competence of enclosed oocytes while modestly affecting mRNA expression of aquaporin and steroidogenic genes in the domestic cat model

et al. 2017

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Using the domestic cat as a non-rodent, larger animal model, the objective of the present study was to determine the impact of a brief incubation in a hypertonic microenvironment on (1) ovarian follicle and oocyte growth in vitro, (2) developmental capacity of the resident oocyte, and (3) expression of aquaporin (AQP) genes in parallel with genes involved in the regulation of folliculogenesis. In Study 1: Secondary or early antral follicles encapsulated in alginate were allocated to one of three treatment groups: 1) culture in standard medium at 290 mOsm for 15 d (Control); 2) incubation in 350 mOsm medium for 1 h followed by culture in standard medium for 15 d (Hypertonic-1h); or 3) incubation in 350 mOsm medium for 24 h followed by incubation in standard medium for additional 14 d (Hypertonic-24h). After measuring follicle and oocyte diameters on Day 15, in vitro-grown oocytes were incubated for 24 h before assessing their nuclear status. In Study 2: secondary or early antral follicles were subjected to one of the three treatments: 1) culture in standard medium at 290 mOsm for 48 h; 2) incubation in 350 mOsm medium for 1 h followed by culture in standard medium for additional 47 h; or 3) incubation in 350 mOsm medium for 24 h followed by culture in standard medium for additional 24 h. At the end of the culture period, all follicles were assessed for mRNA level of Cyp17a1, Cyp19a1, Star (Steroidogenesis genes)AQP1, 3, 5, 7 and 8 as well as Fshr using qPCR. Freshly collected follicles also were subjected to gene expression analysis and served as ‘Non-cultured control’. Hypertonic-24h follicles grew larger (P < 0.05) than the control, whereas those in Hypertonic-1h group exhibited intermediate growth, especially when the culture started at the early antral stage. Oocytes in the Hypertonic-24h group were larger and resumed meiosis at a higher rate than in the other treatments. In vitro culture affected (P < 0.05) mRNA expression of Cyp19a1, Star, AQP1 and AQP7 in both secondary and early antral stage while FSHr was only affected in the former compared to the non-cultured control. Pre-incubating follicles in 350 mOsm medium for 24 h enhanced (P < 0.05) Star and AQP7 while decreasing (P < 0.05) AQP1 expression compared to the control in secondary follicles, but not in the early antral stage. In summary, short-term hypertonic exposure promoted cat follicle development in vitro (including the meiotic competence of the enclosed oocyte) possibly through a mechanism that does not involve water transport genes.

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“…Follicle culture at different stages of development has been used to study diverse biological processes (folliculogenesis, steroidogenesis, and oocyte maturation) in various species, such as goat [9,10], mouse [11][12][13], rat [14], sheep [15,16], dog [17][18][19], and monkey [20][21][22][23]. Regarding the domestic cat, efforts to date have focused on oocytes [7,[24][25][26][27][28][29][30] or preantral follicle cultures [19,31].…”

Section: Introductionmentioning

confidence: 99%

Felis catus ovary as a model to study follicle biology in vitro

Rojo

Linari

Musse

et al. 2015

J Assist Reprod Genet

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Purpose The current study was designed to evaluate the response of individual intact antral follicles from adult female domestic cats to a luteinizing hormone (LH) stimulus in vitro by assessing cumulus-oocyte expansion (C-OE) and steroid production. Methods C-OE and steroid levels (estradiol [E2] and progesterone [P4]) obtained from individual antral feline follicles (n=366 follicles; n=56 cats) were analyzed after 12 or 24 h of culture in the presence or absence of LH (low [3.4 ng/ml] or high [100 ng/ml]). Results At the end of the culture, the highest percentage of expanded cumulus-oocyte complexes (COCs) was observed in the LH groups at 12 or 24 h in comparison to their controls (p<0.001). There was a significant increase in expanded COCs when comparing LH concentrations (high vs. low) at 12 or 24 h. Higher levels of both E2 and P4 were observed in the media from antral follicles after 12 and 24 h of culture in the presence of LH (both concentration, p<0.05). There was no association between hormone levels and follicle diameter; high variability was observed in the steroid levels produced by antral follicles within all treatment groups.Conclusions These data indicate, for the first time, that feline antral follicles (0.5-2 mm) from different stages of the natural estrous cycle can be cultured and will respond to an LH stimulus, based on an increase in steroid levels as well as C-OE after 12 or 24 h in culture.

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In vitro development of secondary follicles from pre-pubertal and adult goats cultured in two-dimensional or three-dimensional systems

Cited by 52 publications

References 28 publications

Size-specific follicle selection improves mouse oocyte reproductive outcomes

Size-specific follicle selection improves mouse oocyte reproductive outcomes

Short-term hypertonic exposure enhances in vitro follicle growth and meiotic competence of enclosed oocytes while modestly affecting mRNA expression of aquaporin and steroidogenic genes in the domestic cat model

Felis catus ovary as a model to study follicle biology in vitro

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