In vitro effect of autologous platelet‐rich plasma on H2O2‐induced oxidative stress in human spermatozoa

Bader, Rainer; Ibrahim, J. N.; Moussa, Mayssam; Mourad, Ali; Azoury, J.; Alaaeddine, Nada

doi:10.1111/andr.12648

Cited by 39 publications

(30 citation statements)

References 47 publications

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“…Different concentrations of H 2 O 2 were added separately to simulate the high-ROS environment observed in H-DFI sperm. 57 Protein expression in the H 2 O 2 -treated sperm was examined. Treatment with different concentrations of H 2 O 2 revealed a gradual decrease in the expression of AKAP4 and PKARII and a gradual increase in the expression of SPA17.…”

Section: Discussionmentioning

confidence: 99%

Decreased AKAP4/PKA signaling pathway in high DFI sperm affects sperm capacitation

Zhang,

Xu,

et al. 2023

Asian Journal of Andrology

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The sperm DNA fragmentation index (DFI) is a metric used to assess DNA fragmentation within sperm. During in vitro fertilization-embryo transfer (IVF-ET), high sperm DFI can lead to a low fertilization rate, poor embryo development, early miscarriage, etc. A kinase anchoring protein (AKAP) is a scaffold protein that can bind protein kinase A (PKA) to subcellular sites of specific substrates and protects the biophosphorylation reaction. Sperm protein antigen 17 (SPA17) can also bind to AKAP. This study intends to explore the reason for the decreased fertilization rate observed in high sperm DFI (H-DFI) patients during IVF-ET. In addition, the study investigates the expression of AKAP, protein kinase A regulatory subunit (PKARII), and SPA17 between H-DFI and low sperm DFI (L-DFI) patients. SPA17 at the transcriptional level is abnormal, the translational level increases in H-DFI patients, and the expression of AKAP4/PKARII protein decreases. H2O2 has been used to simulate oxidative stress damage to spermatozoa during the formation of sperm DFI. It indicates that H2O2 increases the expression of sperm SPA17 protein and suppresses AKAP4/PKARII protein expression. These processes inhibit sperm capacitation and reduce acrosomal reactions. Embryo culture data and IVF outcomes have been documented. The H-DFI group has a lower fertilization rate. Therefore, the results indicate that the possible causes for the decreased fertilization rate in the H-DFI patients have included loss of sperm AKAP4/PKARII proteins, blocked sperm capacitation, and reduced occurrence of acrosome reaction.

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Section: Discussionmentioning

confidence: 99%

Decreased AKAP4/PKA signaling pathway in high DFI sperm affects sperm capacitation

Zhang,

Xu,

et al. 2023

Asian Journal of Andrology

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“…Intracellular ROS levels in spermatozoa were assessed using the nitroblue tetrazolium (NBT) test 38 . Briefly, 0.1% of NBT was added to the spermatozoa (v/v) and incubated for 30 min at 37°C.…”

Section: Methodsmentioning

confidence: 99%

“…Intracellular ROS levels in spermatozoa were assessed using the nitroblue tetrazolium (NBT) test. 38 Briefly, 0.1% of NBT was added to the spermatozoa (v/v) and incubated for 30 min at 37°C. After this incubation period, sperm cells were smeared on a glass slide, air-dried, and stained using a Wright stain.…”

Section: Intracellular Reactive Oxygen Speciesmentioning

confidence: 99%

“…A systematic search of published studies was conducted in the PubMed and Ovid Embase databases for studies published from December 2019 to 18 August 2020 in accordance with PRISMA. 38 All titles or abstracts of English-language studies were reviewed for eligibility. Citations and references of the retrieved studies were used as additional sources.…”

Section: Me Thodsmentioning

confidence: 99%

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Differential impact of four sperm preparation techniques on sperm motility, morphology, DNA fragmentation, acrosome status, oxidative stress, and mitochondrial activity: A prospective study

Raad

Bakos

Bazzi³

et al. 2021

Andrology

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Background: Suboptimal human semen handling in vitro may induce sperm damage.However, the effects of semen swim-up, pellet swim-up, density gradient, and density gradient followed by SU on sperm motility, morphology, DNA fragmentation, acrosome reaction, intracellular reactive oxygen species, and mitochondrial activity were not fully understood.Objectives: To study the impact of four sperm preparation techniques on sperm functional parameters. Materials and methods:This study was conducted on 60 infertile men with a minimum sperm concentration of 20 × 10 6 /ml and total sperm motility of ≥30%. Each raw semen sample was divided into four aliquots. Each aliquot was prepared by one of the tested techniques. Various sperm characteristics were assessed before and after sperm preparation.Results: Density gradient and density gradient followed by SU resulted in significantly higher DNA fragmentation percentages compared with semen swim-up (p < 0.001 and p < 0.001, respectively) and pellet swim-up (p < 0.001 and p < 0.001, respectively). Significantly higher percentages of spermatozoa with intact acrosome were detected in semen swim-up (p < 0.001) and pellet swim-up (p < 0.001) compared with raw semen. The percentage of reactive oxygen species-positive spermatozoa was significantly higher after pellet swim-up (p < 0.001), density gradient (p < 0.001), and density gradient followed by SU (p < 0.001) than raw semen. In addition, the percentages of 100% stained midpiece (active mitochondria) were significantly higher in semen swim-up (p < 0.001) and pellet swim-up (p < 0.001) compared with raw semen. Discussion and conclusion:To the best of our knowledge, this is the first report comparing the impact of these techniques on various sperm functional parameters.Semen swim-up was more effective than density gradient in selecting better spermatozoa in terms of DNA integrity, reactive oxygen species levels, acrosome status, and

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“…A high DNA fragmentation index (DFI) (DFI ≥ 30%) reduces the rate of biochemical pregnancy, the clinical pregnancy rate of frozen embryo transfer (FET), and the delivery rate of fresh embryo transfer [ 5 ]. Autologous platelet-rich plasma (PRP) is used at a 2% concentration to decrease ROS, significantly improving sperm parameters, reducing ROS and DNA fragmentation, and improving motility [ 6 ]. PRP is often used to treat infertility in females but is rarely utilized for men with similar issues.…”

Section: Introductionmentioning

confidence: 99%

Overcoming Male Factor Infertility: A Journey Through Assisted Reproductive Technology With Platelet-Rich Plasma Therapy

Ulhe,

Choudhary,

Shrivastava

et al. 2024

Cureus

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This case study presents a couple’s journey through assisted reproductive technology (ART) experiencing two failed in vitro fertilization cycles. The couple underwent a comprehensive examination, revealing the normal parameters for the female, but asthenoteratozoospermia in the male indicating high morphological defects and reduced sperm motility. Subsequently, intracytoplasmic sperm injection (ICSI) was planned. Despite retrieving six oocytes during ovum pickup (OPU), all blastocysts stopped growth on the second day, prompting a sperm chromatin test disclosing highly DNA-fragmented sperm. Platelet-rich plasma (PRP) therapy was initiated to improve sperm quality, along with frozen embryo transfer (FET). Sperm were incubated with PRP, yielding improved sperm motility and reduced sperm DNA fragmentation. OPU yielded five good-quality metaphase II (MII) oocytes, which were successfully fertilized with PRP-treated sperm, resulting in the formation of four blastocysts. These blastocysts were frozen and later used for FET, resulting in a positive pregnancy outcome and successful conception. This case highlights the importance of personalized intervention in addressing the infertility factor in males and achieving successful ART outcomes.

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In vitro effect of autologous platelet‐rich plasma on H₂O₂‐induced oxidative stress in human spermatozoa

Cited by 39 publications

References 47 publications

Decreased AKAP4/PKA signaling pathway in high DFI sperm affects sperm capacitation

Decreased AKAP4/PKA signaling pathway in high DFI sperm affects sperm capacitation

Differential impact of four sperm preparation techniques on sperm motility, morphology, DNA fragmentation, acrosome status, oxidative stress, and mitochondrial activity: A prospective study

Overcoming Male Factor Infertility: A Journey Through Assisted Reproductive Technology With Platelet-Rich Plasma Therapy

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