In vitro effects of four polysaccharides containing β-D-Glu p on intestinal function

Abstract

Plant polysaccharides exert a wide range of biological effects on the intestinal function. In this study, four polysaccharides containing β-D-Glup, including Dendrobium officinale polysaccharide (DOP), Dendrobium aphyllum polysaccharide (DAP), and glucans from yeast (G-Y) and barley (G-B), were investigated in vitro for their intestinal-promoting functions such as inhibition of digestive enzymes, effect of blood glucose and blood lipid levels by using ultraviolet spectrophotometry, as well as the relationship of their structures to those functions. The results indicated that all polysaccharides significantly slowed glucose diffusion rate, decreased protease, and pancreatic lipase activities. Increased branching degrees had a deleterious effect on the α-amylase activity but increased bile acid binding. Increased calcium concentration in glucans (G-Y and G-B) and increased mannose molar ratio in Dendrobium polysaccharides (DOP and DAP) were correlated with increased pancreatic lipase inhibition. The polysaccharides with higher glucose content and branching degrees had higher bile acid binding and disruption of cholesterol micelle formation. These polysaccharides exerted a variety of potential health-promoting effects attributed to their different structures, which should be further investigated in vivo.

New Water Soluble Polysaccharide From Periploca Laevigata: Functional Characterization and Evaluation of Multi-biological Activities.

“…Furthermore, enzyme activity was decreased in concentration depending-manner. The obtained results were in agreement with several works reported in the literature with regards to the anti-protease effects of polysaccharides 42,43 .…”

“…It was reported that there was a relationship between polysaccharide viscosity, protease activity 43 and reduction of protein digestibility 44 . Therefore, there may be multiple mechanisms by which polysaccharides affect protease activity.…”

“…In a recent study, Wang et al 43 reported that there may be multiple ways in which polysaccharides affect protease activity and protein digestibility. The decrease in proteolytic activity may have been due to the ability of polysaccharide to reduce protein digestibility to some extent, possibly because of their monosaccharide compositions.…”

Physicochemical Characteristics, Anti-Lipase and Antioxidant Activities of Polysaccharide Extracted from Astragalus spinosis Grown in the Northern Region of Saudi Arabia

“…The lipase activity was totally inhibited at 2.5 mg and 3.0 mg/mL. These results are similar to those found by [39]. The antioxidant activity of polysaccharide from plant extracts have received an extensive study and attention.…”

“…The inhibitory capacities of the polysaccharide on pancreatic lipase were evaluated [18]. Briefly, various concentrations of the polysaccharide solution (0.5, 0.75, 1.0, 1.5, 2.0, 2.5, 3.0 mg/L, 1.0 mL), soybean oil as a substrate (1.0 mL), 5 mL phosphate buffer solution (0.1 mol/L, pH 7.2) and 1 mL of lipase solution (0.71 mg/mL, pig pancreatic Type II) were combined and incubated at 37°C for 1 h. The mixture was transferred to a boiling water bath for 5 min and then centrifuged (4800 g, 10 min) (L600, Cence Test Instrument Co., Ltd., Hunan, China).…”