<i>In vitro</i> effects of nonesterified fatty acids and β-hydroxybutyric acid on inflammatory cytokine expression in bovine peripheral blood leukocytes

Li, Chengyan; Liao, Yu-Wen; Liu, Chia-So; Cheng, C. K.; Chan, Jacky Peng-Wen; Wang, Chia-Woei

doi:10.1080/1828051x.2021.2006806

Cited by 5 publications

(2 citation statements)

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“…Multiparous dairy cows with greater IGF‐I concentrations had higher first‐service conception rate (Patton et al, 2007) and conceived earlier (Wathes, Bourne, et al, 2007). An important feature of nonadaptive high‐producing dairy cows is high blood NEFA concentrations (Jorritsma et al, 2003), which may have negative impacts on fertility (Canfield & Butler, 1990), probably due to inflammation induced by NEFA (Fu et al, 2014; LeBlanc, 2014; Sordillo & Raphael, 2013) probably inducing inflammation through the expression of related genes (Gai et al, 2019; Li et al, 2021; Sun et al, 2020). Greater NEFA and less IGF‐I concentrations have been associated with impaired reproductive performance in dairy cows (Abdelli et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Effect of body condition loss in early lactation Holstein cows on plasma IGF‐I, prolactin and NEFA during breeding period and its association with some reproductive indices

Mirzaei

Mohebbi‐Fani

Omidi

et al. 2023

Reprod Domestic Animals

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The associations between early lactation changes in the body condition score (BCS) and mid‐lactation levels of blood insulin‐like Growth Factor‐I (IGF‐1), prolactin, insulin, nonesterified fatty acids (NEFA), milk production and some reproductive indices were investigated. Monthly milk yield and BCS were recorded. Blood samples were collected on days 60, 90, and 120 (±5 days) of lactation. Twelve and 17 cows showed BCS losses ≤0.75 and >0.75 on day 60, respectively. BCS losses ≤0.75 till day 60 after calving enhanced the IGF‐I concentration on day 90 (57.8 ± 10.7 vs. 35.6 ± 17.8 ng/mL; p = .001). The differences in blood metabolites were prominent on day 90. Trends of insulin changes between day 60 BCS (p = .04) groups were different. Inverse correlations were detected between IGF‐I and NEFA (−0.6, p = .001), IGF‐I and milk production (−0.4, p = .05), NEFA and prolactin (−0.8, p < .001) on day 90. Higher percentages of cows with BCS loss >0.75 (day 60) showed their first heat after day 40 postpartum. In conclusion, irrespective of BCS at the time of breeding, the level of BCS loss during the first 60 days of lactation may affect further reproductive performance due to prolonged alterations of IGF‐I, NEFA, prolactin and insulin levels. The extent of postpartum BCS loss may be a predictor of the metabolic status of cows during mid‐lactation.

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Section: Discussionmentioning

confidence: 99%

Effect of body condition loss in early lactation Holstein cows on plasma IGF‐I, prolactin and NEFA during breeding period and its association with some reproductive indices

Mirzaei

Mohebbi‐Fani

Omidi

et al. 2023

Reprod Domestic Animals

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“…Moreover, the immune function of peripheral leukocytes is suppressed during the transition period, involving issues such as decreased cell proliferation, increased secretion of proinflammatory cytokines, and reduced phagocytic activity [6]. Culturing with high concentrations of NEFA results in impaired cell functions for bovine neutrophils [7], induces the expression of pro-inflammatory cytokines in peripheral leukocytes, and intensifies the inflammatory response induced by lipopolysaccharides [8]. Hence, higher NEFA levels may disrupt the immune cell functions of bovine peripheral leukocytes and increase the health risks posed to dairy cows during the transition period.…”

Section: Introductionmentioning

confidence: 99%

The Protective Role of Vitamin E against Oxidative Stress and Immunosuppression Induced by Non-Esterified Fatty Acids in Bovine Peripheral Blood Leukocytes

Li,

Lin,

Moonmanee

et al. 2024

Animals

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High levels of non-esterified fatty acids (NEFAs) during the transition period lead to increased oxidative stress and immunosuppression in cows. Feeding them a vitamin-E-supplemented diet reduces reactive oxygen species (ROS) levels in the blood and diminishes immunosuppression in the transition period. However, whether the restoration of immune cell function occurs through the direct action of vitamin E in cells is still a topic that requires further discussion. Therefore, in this experiment, we aimed to investigate the effect of NEFAs on peripheral blood leukocytes (PBLs) and whether vitamin E mitigates the impact of NEFAs. We employed three groups: (1) blank, (2) NEFA only, and (3) pre-culturing with vitamin E before NEFA treatment (VENEFA). In peripheral blood mononuclear cells (PBMCs), there were no differences in vitamin E content among the three groups. However, in the vitamin E pre-treatment group, the vitamin E levels of polymorphonuclear neutrophils (PMNs) were significantly higher than those in the other two groups. NEFA levels increased malondialdehyde (MDA) levels in PBMCs, but pre-treatment with vitamin E reduced accumulation of MDA levels. Regarding the expression of proinflammatory genes, NEFAs increased the expression of interleukin-1β in PBMCs and colony-stimulating factor 2 in PMNs. Vitamin E pre-treatment restored the increase in interleukin-1β levels caused by NEFAs in PBMCs. None of the groups affected the phagocytosis of PMNs. Few studies have confirmed that NEFAs cause oxidative stress in bovine PBLs. In summary, this study found that NEFAs induce oxidative stress in PBLs and alter the expression of inflammation-related genes; meanwhile, vitamin E can reduce some of the effects caused by NEFAs. This result may suggest that vitamin E can assist bovine PBLs in resisting the immune suppression caused by an NEB during the transition period.

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L-Histidine attenuates NEFA-induced inflammatory responses by suppressing Gab2 expression

Li,

Wang,

Ren

et al. 2024

Life Sciences

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In vitro effects of nonesterified fatty acids and β-hydroxybutyric acid on inflammatory cytokine expression in bovine peripheral blood leukocytes

Cited by 5 publications

References 50 publications

Effect of body condition loss in early lactation Holstein cows on plasma IGF‐I, prolactin and NEFA during breeding period and its association with some reproductive indices

Effect of body condition loss in early lactation Holstein cows on plasma IGF‐I, prolactin and NEFA during breeding period and its association with some reproductive indices

The Protective Role of Vitamin E against Oxidative Stress and Immunosuppression Induced by Non-Esterified Fatty Acids in Bovine Peripheral Blood Leukocytes

L-Histidine attenuates NEFA-induced inflammatory responses by suppressing Gab2 expression

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