In Vitro Effects of Oestrogens, Antioestrogens and SERMs on Pancreatic Solid Pseudopapillary Neoplasm-Derived Primary Cell Culture

Abstract

Background: Solid-pseudopapillary neoplasms of the pancreas (SPNs) are uncommon tumours usually frequent in young women. Although the pathogenesis of SPNs is uncertain a potential influence of the sex hormone milieu on the biology of these tumours has been suggested. The controversial expression of oestrogen receptors (ERs) in SPNs, provide a rationale for studying the effects of oestrogenic molecules on SPN development.Methods: The expression of a large series of hormonal ligands and receptors was evaluated in tissue specimens and in a primary cell culture (SPNC), obtained from a SPN in young female patient. The effects of 17β-oestradiol (17βE2), ICI 182,780 and tamoxifen (Tam) on cell replication and growth were examined.Results: We have established SPNC primary line. Immunocytochemical analysis was positive for vimentin, cyclin D1 and β-catenin and negative for cytokeratin, CD10 and neuroendocrine markers, in line with the immunostaining features of the tumoral tissue. Expression of ERα, ERβ and progesterone mRNAs was demonstrated in SPNC and tumor tissue. A proliferative and antiproliferative action of 17βE2 and Tam respectively were proved in SPNC.Conclusions: In conclusion, we provide the first direct evidence that oestrogenic molecules can influence proliferation of SPNC, offering future strategies in the control of this neoplasia via selective ER modulators.

Sex differences in solid pseudopapillary neoplasm of the pancreas: A population‐based study

“…For estrogen, Tognarini I et al demonstrated the strong expression of ER in tumor tissue and the proliferative action of estrogen in vitro, offering potential treatment strategies for SPN via selective ER modulators. 27 Similarly, another study reported that antiestrogen drugs for cases with unresectable liver metastasis resulted in a favorable prognosis. 28 Therefore, in our study, we presumed that the early onset in women might be attributed to exposure to progesterone and/or estrogen during the reproductive period, while the late onset might be attributed to accumulated lifetime environmental exposure.…”

“…Case reports showed that SPN grew rapidly during pregnancy, 24‐26 and progesterone might act as an oncogenic factor in SPN. For estrogen, Tognarini I et al demonstrated the strong expression of ER in tumor tissue and the proliferative action of estrogen in vitro, offering potential treatment strategies for SPN via selective ER modulators 27 . Similarly, another study reported that antiestrogen drugs for cases with unresectable liver metastasis resulted in a favorable prognosis 28 .…”

The Sex Features of Patients With Solid Pseudopapillary Neoplasms of the Pancreas: A Retrospective Study

“…Moreover, the estrogen receptor was strongly expressed in tumor tissues. The proliferative action of estrogen in vitro was also identified (32). Therefore, the sex features of SPN may be due to exposure to progesterone and/or estrogen during the reproductive period in females (7).…”

Đặc Điểm Hình Ảnh Chụp Cắt Lớp Vi Tính Của U Đặc Giả Nhú Tụy Kích Thước Nhỏ (≤ 3 Cm)

“…This finding suggests that SPTs have relations to female sex hormones. Supporting this hypothesis, the study of Isabella Tognarini et al concluded that estrogenderived molecules could affect the development of SPT cells [7].…”