<i>In vitro</i> effects of two polysaccharide fractions from <i>Laminaria japonica</i> on gut microbiota and metabolome

Lu, Xingyu; Xu, Huji; Fang, Fang; Liu, Juncheng; Wu, Kaizhang; Zhang, Yuwei; Wu, Jihong; Gao, Jie

doi:10.1039/d2fo04085a

Cited by 10 publications

(6 citation statements)

References 44 publications

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“…Furthermore, as shown in Figures 5B,C , compared with the CON group, SFSP significantly reduced the abundance of Firmicutes and promoted the growth of Bacteroidetes ( p < 0.05), resulting in the significantly heighted ratio of Firmicutes phylum to Bacteroidetes phylum (F/B) in SFSP group ( p < 0.05) in Figure 5D . Our results are similar to those of a recent study on the fermentation of sulfated polysaccharides from Laminaria japonica , which the high molecular weight component of sulfated polysaccharides from Laminaria japonica significantly down regulated the value of F/B ( 35 ). This suggests that molecular weight may be an important factor in regulating gut microbiota structure.…”

Section: Resultssupporting

confidence: 91%

Modulation effect of sulfated polysaccharide from Sargassum fusiforme on gut microbiota and their metabolites in vitro fermentation

Jiang,

Song,

et al. 2024

Front. Nutr.

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The present study demonstrated the digestion behavior and fermentation characteristics of a sulfated polysaccharide from Sargassum fusiforme (SFSP) in the simulated digestion tract environment. The results showed that the molecular weight of two components in SFSP could not be changed by simulated digestion, and no free monosaccharide was produced. This indicates that most of SFSP can reach the colon as prototypes. During the fermentation with human intestinal flora in vitro, the higher-molecular-weight component of SFSP was utilized, the total sugar content decreased by 16%, the reducing sugar content increased, and the galactose content in monosaccharide composition decreased relatively. This indicates that SFSP can be selectively utilized by human intestinal flora. At the same time, SFSP also changed the structure of intestinal flora. Compared with the blank group, SFSP significantly increased the abundance of Bacteroidetes and decreased the abundance of Firmicutes. At the genus level, the abundances of Bacteroides and Megamonas increased, while the abundances of Shigella, Klebsiella, and Collinsella decreased. Moreover, the concentrations of total short-chain fatty acids (SCFAs), acetic, propionic and n-butyric acids significantly increased compared to the blank group. SFSP could down-regulate the contents of trimethylamine, piperidone and secondary bile acid in fermentation broth. The contents of nicotinic acid, pantothenic acid and other organic acids were increased. Therefore, SFSP shows significant potential to regulate gut microbiota and promote human health.

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Section: Resultssupporting

confidence: 91%

Modulation effect of sulfated polysaccharide from Sargassum fusiforme on gut microbiota and their metabolites in vitro fermentation

Jiang,

Song,

et al. 2024

Front. Nutr.

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“…The decrease in abundance of Bacteroidota was attributed to the decrease in structural polysaccharides in the rumen caused by the increase in concentrate supplementation. Corroboratively, Bacteroidota are the main degraders of structural polysaccharides in the rumen ( Lu et al, 2023 ).…”

Section: Discussionmentioning

confidence: 99%

Concentrate supplementation improves cold-season environmental fitness of grazing yaks: responsive changes in the rumen microbiota and metabolome

Yi,

Wu,

Liu

et al. 2023

Front. Microbiol.

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Yak (Bos grunniens) is an important economic animal species on the Qinghai-Tibet Plateau. Yaks grazed in the cold season often suffer from nutritional stress, resulting in low production performance. This situation can be improved by properly feeding the grazing yaks in the cold season; however, there is still little information about the effect of different feeding levels on the intestinal microflora and metabolites of yaks. Therefore, this study aimed to explore the effect of feeding different doses of concentrate supplements on rumen bacterial communities and metabolites in grazing yaks during the cold season. Feed concentrate supplementation significantly improved the production performance and rumen fermentation status of grazing yaks during the cold season, and switched the type of ruminal fermentation from acetic acid fermentation to propionic acid fermentation. Ruminal fermentation parameters and ruminal bacterial abundance correlated strongly. At the phylum level, the abundance of Firmicutes increased with increasing concentrate supplementation, while the opposite was true for Bacteroidota. At the genus level, the abundance of Christensenellaceae_R-7_group, NK4A214_group, Ruminococcus, norank_f__Eubacterium_coprostanoligenes_group, norank_f__norank_o__ Clostridia_UCG-014, Lachnospiraceae_NK3A20_group, Acetitomaculum, and Family_XIII_AD3011_group increased with increasing concentrate supplementation, while the abundance of Rikenellaceae_RC9_gut_ group decreased. Dietary concentrate supplementation altered the concentration and metabolic mode of metabolites in the rumen, significantly affecting the concentration of metabolites involved in amino acid and derivative metabolism (e.g., L-aspartic acid, L-glutamate, and L-histidine), purine metabolism (e.g., guanine, guanosine, and hypoxanthine), and glycerophospholipid metabolism (e.g., phosphatidate, phosphatidylcholine, and phosphocholine), and other metabolic pathways. The strong correlation between yak rumen microorganisms and metabolites provided a more comprehensive understanding of microbial community composition and function. This study showed significant changes in the composition and abundance of bacteria and metabolites in the rumen of cool season grazing yaks fed with concentrate supplements. Changes in ruminal fermentation parameters and metabolite concentration also showed a strong correlation with ruminal bacterial communities. These findings will be helpful to formulate supplementary feeding strategies for grazing yaks in the cold season from the perspective of intestinal microorganisms.

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“…The feces of high-glucose mice (C57BL/6 N) were purchased from Honfer White Rat Breeding Export Base (Tsingtao China) to simulate the intestinal environment of animals ( Fan J. et al, 2022 ; Fan Y. et al, 2022 ). Samples of 2 g of clean feces from 5 high-sugar obese mice (blood sugar content >300 mg 100 mL −1 ) and 2 g of clean feces from 5 normal mice were collected and immediately transferred to sterile centrifuge tubes and stored at −80°C for future use within 2 h ( Lu et al, 2023 ). The fecal samples used in this study were obtained from 7-week-old male mice.…”

Section: Methodsmentioning

confidence: 99%

Lonicera caerulea L. polyphenols improve short-chain fatty acid levels by reshaping the microbial structure of fermented feces in vitro

Cao,

Wang,

Ren

et al. 2023

Front. Microbiol.

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Increasing evidence suggests that the pathogenesis of type 2 diabetes mellitus (T2DM) is closely related to the gut microbiota. Polyphenols have been shown to alleviate T2DM, but the effects of L. caerulea L. polyphenols (LPs) on the gut microbiota and metabolites remain elusive. In this study, the inhibitory effects of fermented L. caerulea L. polyphenols (FLPs) and unfermented L. caerulea L. polyphenols (ULPs) on α-amylase and α-glucosidase and the impact of LP on the gut microbiota and metabolites were investigated. Furthermore, the relationship between the two was revealed through correlation analysis. The results showed that ULP and FLP had the highest inhibitory rates against α-amylase and α-glucosidase at 4 mg ml−1, indicating a strong inhibitory ability. In addition, LP plays a regulatory role in the concentration of short-chain fatty acids (SCFAs) and tends to restore them to their normal levels. LP reversed the dysbiosis of the gut microbiota caused by T2DM, as evidenced by an increase in the abundance of bacterial genera such as Lactobacillus, Blautia, and Bacteroides and a decrease in the abundance of bacterial genera such as Escherichia-Shigella and Streptococcus. Similarly, after LP intervention, the relationships among microbial species became more complex and interconnected. In addition, the correlation between the gut microbiota and metabolites was established through correlation analysis. These further findings clarify the mechanism of action of LP against T2DM and provide a new target for T2DM interventions.

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In vitro effects of two polysaccharide fractions from Laminaria japonica on gut microbiota and metabolome

Abstract: To investigate the prebiotic potential of two Laminaria japonica polysaccharides (LJP) fractions with different molecular weights and structures, we conducted in vitro simulated digestion and fermentation with hyperlipidemia-associated human gut...

Cited by 10 publications

References 44 publications

Modulation effect of sulfated polysaccharide from Sargassum fusiforme on gut microbiota and their metabolites in vitro fermentation

Modulation effect of sulfated polysaccharide from Sargassum fusiforme on gut microbiota and their metabolites in vitro fermentation

Concentrate supplementation improves cold-season environmental fitness of grazing yaks: responsive changes in the rumen microbiota and metabolome

Lonicera caerulea L. polyphenols improve short-chain fatty acid levels by reshaping the microbial structure of fermented feces in vitro

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