<i>In Vitro</i>
            Evaluation of Tellurium-Based AS101 Compound against Neisseria gonorrhoeae Infectivity

Yang, Tsung-Ying; Tseng, Sung-Pin; Ho, Han‐Chen; Chen, Li-Hsuan; Hsueh, Po‐Ren; Lu, Po‐Liang; Lin, Chia-Hsuan; Wang, Liangchun

doi:10.1128/spectrum.01496-22

Cited by 5 publications

(2 citation statements)

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“…Our previous work has evaluated the effects of a tellurium-based inorganic compound (AS101) on GC [ 37 ]. Comparing teleost-derived AMP to AS101, we found that the teleost-derived AMP possess more bactericidal properties than AS101, as shown in the time-kill curve and TEM, thus suggesting that less antibiotic resistance could occur using AMP.…”

Section: Discussionmentioning

confidence: 99%

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The Evaluation of Teleost-Derived Antimicrobial Peptides Against Neisseria gonorrhoeae

Huang,

Liou,

Lee

et al. 2024

Cureus

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Introduction Gonorrhea has become an emerging sexually transmitted infection worldwide. The multi-antibiotic resistance facilitates the transmission; thus, new antibiotics or alternatives are needed. Antimicrobial peptides (AMP) are antimicrobials naturally secreted by the host as a defense material. Teleost-derived AMP have gained attention over the past two decades due to their potent efficacy toward microorganisms. This study examines teleost-derived AMP against Neisseria gonorrhoeae (GC), the responsible bacteria for gonorrhea, to evaluate the antibiotic potential as a future alternative for preventing gonorrhea. Methods Minimal inhibitory concentration (MIC) and time-killed assay were conducted to evaluate the inhibition concentration of each AMP. Transmission electron microscopy was used to confirm the potential mode of action. The inhibition of microcolony formation and adherence to epithelial cells were examined to assess the infection inhibition. Results Pardaxin-based (flatfish pardaxin {PB2}) and piscidin-based (striped bass piscidin 1 {PIS} and tilapia piscidin {TP} 4) AMP were effective toward GC under or equal to 7.5 μg/mL as of minimal inhibitory concentration. Transmission electron microscopy images revealed that these AMP attack bacterial membranes as membrane blebbing and breakage were observed. These AMP also effectively reduced the GC biofilm formation, as well as their adherence to human endocervical epithelial cells. Conclusion Pardaxin-based (PB2) and piscidin-based (PIS and TP4) teleost-derived AMP can inhibit GC and potentially serve as the new antibiotic alternative for preventing GC colonization and infection. This study will shed some light on the future development of teleost-derived AMP in treating gonorrhea and maintaining reproductive health.

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Section: Discussionmentioning

confidence: 99%

“…Our previous work has evaluated the effects of a tellurium-based inorganic compound (AS101) on GC [37].…”

Section: Discussionmentioning

confidence: 99%