<i>In vitro</i> evaluation of the genotoxic and cytotoxic effects of artesunate, an antimalarial drug, in human lymphocytes

Mota, Tatiane Cristina; Cardoso, Plínio C S; Gomes, Lorena Monteiro; Vieira, Priscilla Cristina Moura; Corrêa, Regianne M. S.; Santana, Priscila Di Paula Bessa; Miranda, M. S.; Burbano, Rommel Rodrı́guez; Bahia, Marcelo de Oliveira

doi:10.1002/em.20659

Cited by 21 publications

(39 citation statements)

References 23 publications

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“…Artesunate, is a derivative of artemisinin isolated from Artemisia annua L, has been used to treat malaria, especially against cerebral malaria (Padilla-Camberos et al, 2010;Bachmeier et al, 2011;Berdelle et al, 2011;Jin et al, 2011;Mota et al, 2011;Jiang et al, 2012;Zhou et al, 2012), new report shown that it has a variety of biological activities, such as hepatoprotective, antioxidative, antiinflammatory, antidiabetic, antiallergic, and antibacterial effects (Li et al, 2009;Bat-Chen et al, 2010;Du et al,…”

Section: Introductionmentioning

confidence: 99%

The Synergistic Anticancer Effect of Artesunate Combined with Allicin in Osteosarcoma Cell Line in Vitro and in Vivo

Jiang¹,

Huang²,

Wang³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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Background: Artesunate, extracted from Artemisia annua, has been proven to have anti-cancer potential. Allicin, diallyl thiosulfinate, the main biologically active compound derived from garlic, is also of interest in cancer treatment research. This object of this report was to document synergistic effects of artesunate combined with allicin on osteosarcoma cell lines in vitro and in vivo. Methods: After treatment with artesunate and allicin at various concentrations, the viability of osteosarcoma cells was analyzed by MTT method, with assessment of invasion and motility, colony formation and apoptosis. Western Blotting was performed to determine the expression of caspase-3/9, and activity was also detected after drug treatment. Moreover, in a nude mouse model established with orthotopic xenograft tumors, tumor weight and volume were monitored after drug administration via the intraperitoneal (i.p.) route. Results: The viability of osteosarcoma cells in the combination group was significantly decreased in a concentration and time dependent manner; moreover, invasion, motility and colony formation ability were significantly suppressed and the apoptotic rate was significantly increased through caspase-3/9 expression and activity enhancement in the combination group. Furthermore, suppression of tumor growth was evident in vivo. Conclusion: Our results indicated that artesunate and allicin in combination exert synergistic effects on osteosarcoma cell proliferation and apoptosis.

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Section: Introductionmentioning

confidence: 99%

The Synergistic Anticancer Effect of Artesunate Combined with Allicin in Osteosarcoma Cell Line in Vitro and in Vivo

Jiang¹,

Huang²,

Wang³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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“…These authors also concluded that in mammalian cells, artesunate generated damage through direct or indirect oxidative stress, resulting in DNA strand breakage. Mota et al (2011) also reported the genotoxicity of artesunate in human peripheral lymphocytes. The present study confirmed such genotoxicity in vitro for human metabolizing cells, and further studies are necessary to determine whether the generation of ROS could be involved in the genotoxicity of artesunate.…”

Section: Discussionmentioning

confidence: 99%

“…Aquino et al (2011) reported genotoxic effects of artesunate in liver cells of mice at low doses and clastogenic effects at high doses in bone marrow cells of the same animals. Li et al (2008) and Mota et al (2011) reported genotoxic and cytotoxic effects of artesunate in vitro in Chinese hamster ovary cells and in human peripheral lymphocytes, respectively. To the best of our knowledge, no genotoxic studies on mammalian cells of artemisinin and no genotoxic studies on metabolizing cells of artemisinin and artesunate have been carried out.…”

Section: Introductionmentioning

confidence: 99%

Genotoxic evaluation of the antimalarial drugs artemisinin and artesunate in human HepG2 cells and effects on CASP3 and SOD1 gene expressions

Aquino

Tsuboy²,

Marcarini³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. The malaria treatment recommended by the World Health Organization involves medicines derived from artemisinin, an active compound extracted from the plant Artemisia annua, and some of its derivatives, such as artesunate. Considering the lack of data regarding the genotoxic effects of these compounds in human cells, the objective of this study was to evaluate the cytotoxicity and genotoxicity, and expressions of the CASP3 and SOD1 genes in a cultured human hepatocellular liver carcinoma cell line (HepG2 cells) treated with artemisinin and artesunate. We tested concentrations of 2.5, 5, 7.5, 10, and 20 mg/mL of both substances with a resazurin cytotoxicity assay, and the concentrations used in the genotoxicity experiments (2.5, 5, and 10 mg/mL) and gene expression analysis (5 mg/mL) were determined. The results of the comet assay in cells treated with artemisinin and artesunate showed a significant dosedependent increase (P < 0.001) in the number of cells with DNA damage at all concentrations tested. However, the gene expression analysis revealed no significant change in expression of CASP3 or SOD1. Our data showed that although artemisinin and artesunate exhibited genotoxic effects in cultured HepG2 cells, they did not significantly alter expression of the CASP3 and SOD1 genes at the doses tested.

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“…Genotoxicity and cytotoxicity of artisunate were also studied by Mota et al, 52 in human lymphocyte. Comet assay and micronucleus test were used to evaluate the possible genotoxic effects of artesunate.…”

Section: Artimisininmentioning

confidence: 99%

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In vitro evaluation of the genotoxic and cytotoxic effects of artesunate, an antimalarial drug, in human lymphocytes

Cited by 21 publications

References 23 publications

The Synergistic Anticancer Effect of Artesunate Combined with Allicin in Osteosarcoma Cell Line in Vitro and in Vivo

The Synergistic Anticancer Effect of Artesunate Combined with Allicin in Osteosarcoma Cell Line in Vitro and in Vivo

Genotoxic evaluation of the antimalarial drugs artemisinin and artesunate in human HepG2 cells and effects on CASP3 and SOD1 gene expressions

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