In vitro evaluation of the antibacterial activity of Arctium lappa as a phytotherapeutic agent used in intracanal dressings

Abstract

The discovery of natural biocomponents from plants with antibacterial activity on endodontic microbiota may lead to new therapies. This study evaluated the antibacterial activity of a phytotherapeutic agent prepared from an ethyl acetate fraction (AcOEt) extracted from Arctium lappa. This agent was compared with calcium hydroxide as an intracanal dressing. Twenty-seven maxillary canines were instrumented, sterilized and inoculated with a mixed bacterial suspension of Pseudomonas aeruginosa, Escherichia coli, Lactobacillus acidophilus, Streptococcus mutans and Candida albicans. The teeth were divided into three groups and their canals filled with: group 1, calcium hydroxide and propylene glycol; group 2, a paste containing AcOEt fraction of A. lappa and propylene glycol; group 3, propylene glycol (control). At 7, 14 and 30 days, three teeth from each group were opened and a paper point was placed in the root canal for 5 min. The paper points were transferred to Petri dishes with Brain Heart Infusion (BHI). The bacterial growth was classified. Mild bacterial growth was found in group 1 at all time intervals; in group 2 there was severe growth at 7 days, but no growth at 14 and 30 days. The phytotherapeutic agent extracted from an AcOEt fraction of A. lappa inhibited the growth of all the microorganisms in this study.

Comparison of the Immunomodulatory Properties of Root and Leaves of Arctium Lappa (Burdock) in Vitro

“…Burdock leaves also has been used therapeutically for treatment of bacterial infections [6], attenuating excessive nitric oxide (NO) generation at inflammatory sites [7], and improvement of skin problems in eczema, acne and psoriasis [1].…”

Phytomedicine - A Holistic Approach in Dentistry

“…It has anti-bacterial and antifungal , diuretic, anti-oxidan, anxiolytic action, platelet anti-aggregating effect and HIV-inhibitory action. In dentistry, Arctiumlappa has been investigated due to its antimicrobial potential against oral microorganisms, specifically those associated with endodontic infections [9,10].…”

Antibacterial effect of chlorhexidine-cetrimide combination, <i>Salvia officinalis</i> plant extract and octenidine in comparison with conventional endodontic irrigants

“…Most of the irrigation solutions commonly used in Antibacterial effect of chlorhexidine-cetrimide combination, Salvia officinalis plant extract and octenidine in comparison with conventional endodontic irrigants endodontic treatment still have a number of issues, which means that an alternative solution with good antimicrobial activity, less toxicity and better tissue dissolving properties is still being researched. The role of natural extracts for endodontic purpose has been researched for plants such as Arctium lappa, Morinda citrifolia, Triphala, Green Tea Polyphenols, Liquorice, Ocimum sanctum, Cinnamomum zeylanicum and Syzygium aromaticum due to their antimicrobial efficacy against E. faecalis [22][23][24][25][26] . Salvia officinalis, commonly known as sage, belongs to the Lamiaceae (Labiatae) family.…”