<i>In vitro</i> evaluation of the fermentation properties and potential prebiotic activity of caprine cheese whey oligosaccharides in batch culture systems

Oliveira, Diana; Costabile, Adele; Wilbey, R. A.; Grandison, Alistair S.; Duarte, Luís C.; Roseiro, Luísa B.

doi:10.1002/biof.1043

Cited by 30 publications

(24 citation statements)

References 44 publications

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“…The ability of B. infantis ATCC 15697 to use multiple specific carbohydrate structures (2′FL, LNT, 3′SL, 6′SL, DSL, LNH, Sialic Acid, LSTc, LNnT and LNnH) within the pool was also Previous studies have demonstrated statistically significant increases in Bifidobacterium and Bacteroides growth following incubation with GMO [12]. Similarly, GMO has also been shown to increase levels of bifidobacteria using in vitro fermentation models and in in vivo mouse trials [15,39].…”

Section: Prebiotic Effects Of Gmomentioning

confidence: 95%

Bifidobacterium longum subsp. infantis ATCC 15697 and Goat Milk Oligosaccharides Show Synergism In Vitro as Anti-Infectives against Campylobacter jejuni

Quinn

Slattery

Walsh

et al. 2020

Foods

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Bifidobacteria are known to inhibit, compete with and displace the adhesion of pathogens to human intestinal cells. Previously, we demonstrated that goat milk oligosaccharides (GMO) increased the attachment of Bifidobacterium longum subsp. infantis ATCC 15697 to intestinal cells in vitro. In this study, we aimed to exploit this effect as a mechanism for inhibiting pathogen association with intestinal cells. We examined the synergistic effect of GMO-treated B. infantis on preventing the attachment of a highly invasive strain of Campylobacter jejuni to intestinal HT-29 cells. The combination decreased the adherence of C. jejuni to the HT-29 cells by an average of 42% compared to the control (non-GMO treated B. infantis). Increasing the incubation time of the GMO with the Bifidobacterium strain resulted in the strain metabolizing the GMO, correlating with a subsequent 104% increase in growth over a 24 h period when compared to the control. Metabolite analysis in the 24 h period also revealed increased production of acetate, lactate, formate and ethanol by GMO-treated B. infantis. Statistically significant changes in the GMO profile were also demonstrated over the 24 h period, indicating that the strain was digesting certain structures within the pool such as lactose, lacto-N-neotetraose, lacto-N-neohexaose 3 -sialyllactose, 6 -sialyllactose, sialyllacto-N-neotetraose c and disialyllactose. It may be that early exposure to GMO modulates the adhesion of B. infantis while carbohydrate utilisation becomes more important after the bacteria have transiently colonised the host cells in adequate numbers. This study builds a strong case for the use of synbiotics that incorporate oligosaccharides sourced from goat s milk and probiotic bifidobacteria in functional foods, particularly considering the growing popularity of formulas based on goat milk. Foods 2020, 9, 348 2 of 15Listeria monocytogenes, and Clostridium difficile [4][5][6]. Microbe-associated molecular patterns (MAMPs) are recognized by the host s intestinal pattern recognition receptors (PRRs), and these interactions play key roles in the association of pathogens with the intestinal epithelia. Probiotics also express molecular patterns which can recognize the same trans-membrane receptors as the pathogens, thus blocking the sites for pathogenic contact by competitive exclusion and, in some cases, displacing already-attached pathogens [7]. However, the health benefits associated with bifidobacteria are reliant on such strains colonising the host in sufficient numbers [8]. The important step in microbial colonisation of the intestinal epithelium is the attachment of bacterial surface lectins to intestinal sugar structures. Recent studies have suggested that milk oligosaccharides may enhance the specific ability of bifidobacteria to attach to the GI epithelium [9][10][11]. Indeed, our group investigated the ability of goat milk oligosaccharides (GMO) to increase the attachment of Bifidobacterium longum subsp. infantis ATCC 15697 to HT-29 cells. Exposure of the strain ...

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Section: Prebiotic Effects Of Gmomentioning

confidence: 95%

Bifidobacterium longum subsp. infantis ATCC 15697 and Goat Milk Oligosaccharides Show Synergism In Vitro as Anti-Infectives against Campylobacter jejuni

Quinn

Slattery

Walsh

et al. 2020

Foods

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“…Recovery of OSs from caprine milk is an expensive option, whereas it would be more economical to extract them from a by‐product such as cheese whey, as most of the lactose and OSs are transferred into the whey (Oliveira et al . ). Numerous papers and patents deal with OSs in whey, but they mostly refer to specific galactooligosaccharides (GOSs) obtained from lactose by enzymic reaction (transgalactosylation) and do not refer to the natural milk OSs, produced in the lactating mammary gland of domestic species (Oliveira et al .…”

Section: Animal Milk Os As Alternative Sources For Hmomentioning

confidence: 97%

“…Also, recent research work points out to the potential of caprine milk as a source of bioactive compounds, namely for naturally extracted OSs from caprine milk whey and its utilisation as prebiotics (Oliveira et al . ,b) and lactoferrin (Parc et al . ).…”

Section: Future Opportunities With Animal Milks: Whey As Raw Materialsmentioning

confidence: 99%

Milk oligosaccharides: A review

Oliveira

Wilbey

Grandison

et al. 2015

Int J of Dairy Tech

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Milk oligosaccharides (OSs) confer unique health benefits to the neonate. Although human digestive enzymes cannot degrade these sugars, they support specific commensal microbes and act as decoys to prevent the adhesion of pathogenic micro-organisms to gastrointestinal cells. The limited availability of human milk oligosaccharides (HMOs) impedes research into these molecules and their potential applications in functional food formulations. Recent studies show that complex OSs with fucose and N-acetyl neuraminic acid (key structural elements of HMO bioactivity) also exist in caprine milk, suggesting a potential source of bioactive milk OSs suitable as a functional food ingredient.

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“…We have previously shown that a caprine oligosaccharideenriched fraction (CMOF) supported the growth of selected bifidobacterial strains isolated from breast-fed infants, and stimulated in vitro production of acetate and lactate [16]. Oliveira et al (2012) [17] has also demonstrated the prebiotic activity of CMOF in a batch culture system inoculated with adult human feces. The aim of this study was to determine the effects of prenatal consumption of CMOF on the colon microbiota and milk composition of the dams, and the offspring's development.…”

Section: Wwwmnf-journalcommentioning

confidence: 99%

“…Oliveira et al. (2012) has also demonstrated the prebiotic activity of CMOF in a batch culture system inoculated with adult human feces. The aim of this study was to determine the effects of prenatal consumption of CMOF on the colon microbiota and milk composition of the dams, and the offspring's development.…”

Section: Introductionmentioning

confidence: 99%

Prenatal caprine milk oligosaccharide consumption affects the development of mice offspring

Thum

McNabb

Young

et al. 2016

Molecular Nutrition Food Res

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Scope:The composition of the gastrointestinal (GIT) microbiota, particularly in early life, influences the development of metabolic diseases later in life. The maternal microbiota is the main source of bacteria colonising the infant GIT and can be modified by dietary prebiotics. Our objective was to determine the effects of prenatal consumption of prebiotic caprine milk oligosaccharides (CMO) on the large intestine of female mice, milk composition, and offspring's development. Methods and results: C57BL/6 mice were fed either a control diet, CMO diet, or galactooligosaccharide diet from mating to weaning. From weaning, some pups nursed by CMO, GOS, and control-dams were fed the control diet for 30 days. CMO or GOS-fed dams had increased colon length and milk protein concentration compared to control-fed dams. At weaning, pups from CMO-fed dams had increased body weight and colon length and increased proportions of colonic Bifidobacterium spp compared to the pups from control-fed dams. Thirty days after weaning, pups from CMO-fed dams had increased visceral fat weight compared to pups from control-fed dams. Conclusion: Consumption of CMO by the dams during gestation and lactation improved the development of the pups, and the relative abundance of bifidobacteria and butyric acid in the colon, at weaning. Keywords:Caprine milk oligosaccharides / Development / Prebiotic / Pregnancy / Prenatal diet Additional supporting information may be found in the online version of this article at the publisher's web-site

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In vitro evaluation of the fermentation properties and potential prebiotic activity of caprine cheese whey oligosaccharides in batch culture systems

Cited by 30 publications

References 44 publications

Bifidobacterium longum subsp. infantis ATCC 15697 and Goat Milk Oligosaccharides Show Synergism In Vitro as Anti-Infectives against Campylobacter jejuni

Bifidobacterium longum subsp. infantis ATCC 15697 and Goat Milk Oligosaccharides Show Synergism In Vitro as Anti-Infectives against Campylobacter jejuni

Milk oligosaccharides: A review

Prenatal caprine milk oligosaccharide consumption affects the development of mice offspring

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