<i>In Vitro</i>Evaluation of the Genotoxic Activity and Apoptosis Induction of the Extracts of Roots and Leaves from the Medicinal Plant<i>Coccoloba mollis</i>(Polygonaceae)

Tsuboy, Marcela Stefanini Ferreira; Marcarini, Juliana Cristina; Luiz, Rodrigo Cabral; Barros, Iuri Bezerra de; Ferreira, Dalva Trevisan; Ribeiro, Lúcia Regina; Mantovani, Mário Sérgio

doi:10.1089/jmf.2009.0119

Cited by 20 publications

(17 citation statements)

References 31 publications

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“…They are used worldwide as self-prescribed home medicines, especially in developing countries, as an aid in basic health care in 60%–80% of the population (Agra et al , 2008). Of late, they have even become a source of compounds for the pharmaceutical industry (Tsuboy et al , 2010; Rispler and Sara, 2011). As bioactive compounds from plants have many biological activities, the appreciable biodiversity among Brazilian plants presents great potential in the development of new drugs for application in the treatment of human diseases (Balbani et al , 2009).…”

Section: Introductionmentioning

confidence: 99%

Genotoxicity assessment of Copaiba oil and its fractions in Swiss mice

Almeida¹,

Darin²,

Hernandes³

et al. 2012

Genet. Mol. Biol.

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Copaiba oil-resin, extracted from the trunk of Copaifera, and traditionally used in folk medicine in the treatment of various disorders, has been shown to be an effective antiinflamatory, antitumor, antitetanus, antiseptic and anti-blenorrhagea agent. As, there are few studies evaluating its genotoxicity, this aspect of the commercial oil-resin, and its volatile and resinous fractions, were evaluated in mice by comet assay and micronucleus (MN) test. A single dose of oil resin, volatile or resin fractions (500; 1,000 or 2,000 mg/kg b.w.) was administered by gavage. The chemical compositions of Copaiba oil resin and its fractions was analyzed by gas chromatography. According to comet assaying, treatment with either one did not increase DNA damage, and as to MN testing, there was no alteration in the incidence of micronucleated polychromatic erythrocytes. Chromatographic analysis of the oil-resin itself revealed sesquiterpenes, diterpenic carboxylic acid methyl esters and high levels of β-caryophyllene. Thus, it can be assumed that the oil resin and volatile and resinous fractions from the commercial product are not genotoxic or mutagenic.

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Section: Introductionmentioning

confidence: 99%

Genotoxicity assessment of Copaiba oil and its fractions in Swiss mice

Almeida¹,

Darin²,

Hernandes³

et al. 2012

Genet. Mol. Biol.

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“…The results showed that the extract obtained with the roots of C. mollis is more cytotoxic than that obtained with the leaves, and that the decrease in cell viability observed in the MTT assay was a result, at least in part, of induced apoptosis. Both extracts induced DNA damage at a concentration of 20 μg/mL in the comet assay, but genotoxicity was not detected in any of the treatments carried out in the micronucleus test (TSUBOY, 2009). The aim of the present study was to evaluate the ethanolic extracts of root and leaf for toxicity against Artemia salina (brine shrimp larvae), antioxidant activity and phytochemical content.…”

Section: Introductionmentioning

confidence: 90%

Toxicidade, atividade antioxidante e caracterização fitoquímica das raízes e folhas de Coccoloba mollis.

Barros¹,

Fidelis²,

Daniel³

et al. 2010

Semina: Tech. Ex.

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Coccoloba mollis is a plant that is used in medicine in Londrina, Brazil. The root extract showed stronger activity against A. salina (68.5 μg/mL) than did the leaf extract (1342 μg mL), and demonstrated good activity when compared with the positive control (16.24 μg /mL). The antioxidant potential (Diphenylpicrylhydrazyl-DPPH) of root and leaf extracts was comparable to that of commonly used BHT (butylated hydroxytoluene). In the chemical characterization the compounds identified were a mixture of long chain hydrocarbons, carboxyl esters and 3-taraxerone from leaf ethanolic extract and two anthraquinones (emodin and physcion) from the root one. Phytochemical screening using pharmacognostic methodology revealed the presence of flavonoids and tannins in leaves and roots. Anthraquinones only in the roots. While this analysis resulted negative for alkaloids, coumarins, saponins and simple phenolics. We conclude that the identified emodin and physcion in roots extract, corroborate with the anti-stress action and the use for memory loss attributed by popular use of this medicinal plant in the Londrina region.

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“…The results showed that C. mollis roots extract had higher cytotoxic activity than the resulting extract from the leaves and that the alleviation observed in cell viability in the MTT assay was, at least in part, a result of apoptosis induction. In the comet assay both extracts at a concentration of 20 µg/mL induced DNA damage, but with no genotoxicity detected in the micronucleus test with any of the treatments carried out[21].…”

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confidence: 88%

Biological and phytochemical review on the genus Coccoloba (Polygonaceae)

Hakim

Gad

Radwan

et al. 2019

Archives of Pharmaceutical Sciences Ain Shams University

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Polygonaceae is one of the largest medicinal plant families, vastly distributed worldwide, containing around 1,200 species from 48 genera. Most of the species are located in the northern temperate region, while the other species are allocated from the tropics to the arctic. The prime genera in Polygonaceae are Eriogonum which includes 240 species, Rumex with 200 species, Coccoloba with 120 species, Persicaria with 100 species, and Calligonum with 80 species. Coccoloba is one of the most interesting genera of the family Polygonaceae in terms of biological activities and secondary metabolites. Plants of this genus are used worldwide in traditional folk medicine. The review is a comprehensive literature survey on different Coccoloba species regarding their biological activities and their isolated phytochemicals. Different classes of secondary metabolites were isolated from this genus including flavonoids, phenolic acids, tannins, triterpenes, diterpenes, anthraquinones, isochromene, and volatile oils. Crude extracts and isolated compounds of various Coccoloba species displayed diversity in biological activities. Further investigations are required to explore new bioactive compounds and their pharmacological activities.

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In VitroEvaluation of the Genotoxic Activity and Apoptosis Induction of the Extracts of Roots and Leaves from the Medicinal PlantCoccoloba mollis(Polygonaceae)

Cited by 20 publications

References 31 publications

Genotoxicity assessment of Copaiba oil and its fractions in Swiss mice

Genotoxicity assessment of Copaiba oil and its fractions in Swiss mice

Toxicidade, atividade antioxidante e caracterização fitoquímica das raízes e folhas de Coccoloba mollis.

Biological and phytochemical review on the genus Coccoloba (Polygonaceae)

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