<i>In vitro</i> Fertilisation mit transmigriertem (TM) Rindersamen

Rosenkranz, C; Holzmann, A.; Dickie, M. B.

doi:10.1111/j.1439-0531.1994.tb00565.x

Cited by 4 publications

(1 citation statement)

References 13 publications

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“…After IVF (day 0), COCs were removed by repeated pipetting and denuded zygotes were placed into a well of a 4-well plate containing 700 l of CR1-aa medium (Rosenkranz et al, 1993), supplemented with 44 g/ml sodium pyruvate, 14.6 g/ml glutamine, 10 l/ml penicillin-streptomycin, 3 mg/ml bovine serum albumin (BSA), and 310 g/ml glutathione for 3 days (IVC-I). The presumed zygotes were cultured until day 7 of embryonic development (day 1 was the day on which IVC was performed) in the same medium, except that BSA was replaced with 10% (v:v) FBS from day 3 (IVC-II) at 38.5°C under a humidified atmosphere of 5% CO 2 in air.…”

Section: In Vitro Production Of Bovine Embryos and Embryo Transfer(et)mentioning

confidence: 99%

Generation of large pig and bovine blastocysts by culturing in human induced pluripotent stem cell medium

Gao

Jin

et al. 2015

Zygote

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We investigated the effect of human induced pluripotent stem cell (hiPS) medium on porcine somatic cell nuclear transfer and bovine in vitro fertilized early blastocysts, in comparison with North Carolina State University (NCSU)-37 medium and in vitro culture (IVC)-II medium. After 2 days of culture, the diameter of the portion of the blastocyst that was extruded from the zona pellucid dramatically differed between porcine blastocysts cultured in hiPS medium and those cultured in NCSU-37 medium (221.47 ± 38.94 μm versus 481.87 ± 40.61 μm, P < 0.01). Moreover, the diameter of the portion of the blastocyst significantly differed between bovine blastocysts cultured in hiPS medium and those cultured in IVC-II medium (150.30 ± 29.49 μm versus 195.58 ± 41.59 μm, P < 0.01). Furthermore, the total number of cells per porcine and bovine blastocyst was more than two-fold higher in blastocysts cultured in hiPS medium than in those cultured in NCSU-37 medium (44.33 ± 5.28 and 143.33 ± 16.05, P < 0.01) or IVC-II medium (172.12 ± 45.08 and 604.83 ± 242.64, P < 0.01), respectively. These results indicate that hiPS medium markedly improves the quality of porcine and bovine blastocysts.

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Section: In Vitro Production Of Bovine Embryos and Embryo Transfer(et)mentioning

confidence: 99%