IN VITRO FERTILIZATION OF IN VITRO MATURED MINKE WHALE (BALAENOPTERA ACUTOROSTRATA) FOLLICULAR OOCYTES

Fukui, Yutaka; Mogoe, Toshihiro; Ishikawa, Hajime; Ohsumi, Seiji

doi:10.1111/j.1748-7692.1997.tb00647.x

Cited by 28 publications

(37 citation statements)

References 23 publications

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“…In contrast to AI, handling is reduced and more embryos may be produced with the same semen dose. IVM or IVF have been tried in various species such as the mink whale (Balaenoptera acutorostrata) [20], African elephant (Loxodonta africana) [36], gorilla (Gorilla gorilla) [53], and zebra (Equus burchelli, Equus zebra) [47]. In our deer preservation program, we developed a method for repeated immature oocyte recovery on live sika deer hinds by laparoscopic OPU.…”

Section: In Vitro Production Of Embryosmentioning

confidence: 99%

Reproductive biotechnologies for endangered mammalian species

2000

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-Assisted reproductive techniques (gamete cryopreservation, artificial insemination, embryo transfer, and in vitro fertilization) allow to propagate small fragmented populations of wild endangered species or domestic breeds. There are the best way for producing several offspring from selected genitors in order to avoid inbreeding depression. However, few mammalian species have been well studied for their reproductive biology whereas huge differences have been observed between these species. Furthermore, materials, methods and experimental designs have to be adapted for each case and each limiting factor (wildness, poor quantity of biological material, disparate locations). Genome resource banking is currently arising and the most applied reproductive biotechnology remains artificial insemination. Assisted reproductive techniques currently developed in domestic species (intracytoplasmic sperm injection, nuclear transfer) may offer new opportunities for the propagation of endangered species. endangered species / artificial insemination / cryopreservation / in vitro fertilization / embryo Résumé -Biotechnologies de la reproduction appliquées aux mammifères en voie de disparition. Les techniques de reproduction assistée (cryoconservation des gamètes, insémination artificielle, transfert embryonnaire, fécondation in vitro) permettent d'accroître des populations, parfois dispersées géographiquement, d'espèces sauvages ou domestiques en voie de disparition. Ces méthodes sont le meilleur moyen pour produire plusieurs descendants à partir de géniteurs sélectionnés de façon à éviter la consanguinité. La biologie de la reproduction est connue pour peu d'espèces de mammifères alors que de grandes différences ont pourtant été mises en évidence entre ces espèces. En outre, les matériels, les méthodes, ainsi que les schémas expérimentaux doivent être adaptés à chaque cas et pour chaque facteur limitant (animaux sauvages, peu de matériel biologique, populations dispersées). Des banques génétiques sont actuellement en voie de constitution mais la technique la plus utilisée reste l'insémination artificielle. Cependant, des biotechnologies de la reproduction en cours de mise au point chez les animaux domestiques (injection intra-cytoplasmique de spermatozoïde, transfert nucléaire) pourraient offrir de nouvelles possibilités pour l'accroissement de populations menacées. espèces menacées / insémination artificielle / congélation / fécondation in vitro / embryon Reprod. Nutr. Dev. 40 (2000)

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Section: In Vitro Production Of Embryosmentioning

confidence: 99%

Reproductive biotechnologies for endangered mammalian species

2000

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“…Fukui et al [14,15] attempted in vitro fertilization (IVF) of in vitro matured minke whale follicular oocytes using frozen-thawed spermatozoa, but the number of cleaved oocytes following IVF was low (5-6% of inseminated oocytes). This low cleavage rate may be due to the high proportion of morphologically abnormal spermatozoa ascertained in the present study.…”

Section: Referencesmentioning

confidence: 99%

Morphological Observations of Frozen-Thawed Spermatozoa of Southern Minke Whales (Balaenoptera acutorostrata).

Mogoe

Fukui

Ishikawa³

et al. 1998

Journal of Reproduction and Development

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Abstract. The morphology, motility and viability of frozen-thawed southern minke whale (Balaenoptera acutorostrata) spermatozoa was evaluated. The whales used in this study were captured during the period from December 1994 to February 1995 for the Japanese Whale Research Programme under Special Permit in the Antarctic. Each sperm sample recovered from the vasa deferentia of eleven whales was diluted to a 1:9 ratio with Tris (300 mM)-citrate (94.75 mM)-glucose (27.75 mM)-egg yolk (15%; v/v)-glycerol (5%; v/v) diluents. The diluted samples were cooled gradually at 5 C for 2 h, frozen at 80 C and kept in liquid nitrogen. After thawing, sperm motility was evaluated subjectively. Sperm viability was assessed by eosin-nigrosin staining. Sperm abnormality and head morphology were assessed by carbol-fuchsin staining using phase-contrast microscopy. Sperm dimensions were measured using a calibrated projection microscope. Sperm motility and viability respectively varied from 1.0 to 20% (7 out of 11 samples), and 1.0 to 11.6%. Sperm concentration ranged from 13 to 585.3 × 10 6 spermatozoa/ml. The mean proportion of morphologically abnormal spermatozoa was 84.0 ± 6.7%. Seven morphotypes of sperm heads were observed, and the most common types were conical and elliptic in shape. The mean total length of spermatozoa was 56.7 ± 0.5 µm, with their sperm heads measuring 5.2 ± 0.1 µm for length and 3.0 ± 0.0 µm for width; the mean length of sperm tail was 51.7 ± 0.5 µm.

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“…Addition of 10% wFF to IVM medium and adjustment of the osmolarity t o 3 9 0 m O s m p r o v i d e s a b e t t e r a n d m o r e physiological environment for in vitro maturation of common minke whale oocytes, although the osmolarity of wFF in common minke whales has not been examined [5]. Iwayama et al [4] reported that IVM medium containing 50% wFF resulted in a similar proportion (approximately 30%) of matured oocytes after IVM culture for 28-30 h compared with the previous studies using 96-120 h [1][2][3]. The present study compared two durations of IVM culture (30 or 40 h) using medium supplemented with 10% wFF for common minke whale oocytes and found that 40 h of IVM culture was superior to 30 h in terms of the proportion of in vitro matured oocytes.…”

Section: Discussionmentioning

confidence: 94%

“…Immediately after death, the vasa deferentia were carefully retrieved to prevent the possibility of contamination by seawater. The spermatozoa c o l l e c t e d f r o m t h e v a s a d e f e r e n t i a w e r e cryopreserved in liquid nitrogen as described previously [2,9]. Briefly, spermatozoa were diluted 5-fold with a cryopreservation medium (Tris-fructose based), and aliquots (0.5 ml) of the sperm suspension were put in micro-cryotubes.…”

Section: Collection and Cryopreservation Of Whale Spermatozoamentioning

confidence: 99%

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Attempt at Intracytoplasmic Sperm Injection of In Vitro Matured Oocytes in Common Minke Whales (Balaenoptera acutorostrata) Captured During the Kushiro Coast Survey

Fukui

Iwayama

Matsuoka

et al. 2007

Journal of Reproduction and Development

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Abstract. The present study was conducted during the Kushiro Coast Survey in an attempt to produce common minke whale embryos. In Experiment 1, we attempted to determine the appropriate culture duration (30 or 40 h) for in vitro maturation (IVM) of immature oocytes using the Well of the Well method. In Experiment 2, and intracytoplasmic sperm injection (ICSI) was applied to matured oocytes from prepubertal and adult common minke whales after IVM culture (40 or 48 h), and then their embryonic development was assessed. In Experiment 1, the maturation rate of oocytes cultured for 40 h (30.4%) was significantly higher than that of oocytes cultured for 30 h (6.8%; P<0.01). In Experiment 2, a total of 35 and 46 immature oocytes derived from adult (n=2) and prepubertal (n=6) minke whales, respectively, were cultured for 40 or 48 h. The maturation rate in the oocytes from the adult whales (34.2%) tended to be higher than that of the oocytes from the prepubertal whales (19.6%), but there was no significant difference. Following ICSI, 3 out of the 10 inseminated and cultured oocytes from the adult whales cleaved (2-, 8-, and 16-cell stages); all of these oocytes had been matured for 40 in culture. However, these oocytes did not develop to further stages. Only one of the 6 oocytes derived from the prebuertal whales, IVM cultured for 40 h and inseminated, developed to the 4-cell stage. The present results indicate that a 40 h IVM culture produces significantly higher rates of in vitro maturation than a 30 h IVM culture for common minke whale oocytes. Following ICSI, some oocytes cleaved to the 16-cell stage, but no further development was observed.

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IN VITRO FERTILIZATION OF IN VITRO MATURED MINKE WHALE (BALAENOPTERA ACUTOROSTRATA) FOLLICULAR OOCYTES

Cited by 28 publications

References 23 publications

Reproductive biotechnologies for endangered mammalian species

Reproductive biotechnologies for endangered mammalian species

Morphological Observations of Frozen-Thawed Spermatozoa of Southern Minke Whales (Balaenoptera acutorostrata).

Attempt at Intracytoplasmic Sperm Injection of In Vitro Matured Oocytes in Common Minke Whales (Balaenoptera acutorostrata) Captured During the Kushiro Coast Survey

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