In Vitro Flower Induction from Shoots Regenerated from Cultured Axillary Buds of Endangered Medicinal Herb Swertia chirayita H. Karst.

Abstract

In vitro flowering and effective micropropagation protocol were studied in Swertia chirayita, an important medicinal plant using axillary bud explants. The Murashige and Skoog's medium (MS) supplemented with benzyl amino purine (BAP) 1.0 mg L−1 and adenine sulfate 70.0 mg L−1 was found optimum for production of multiple shoots. In the present study, incubation of flowering cultures on BAP supplemented medium (during shoot multiplication) was found necessary for flowering (6 weeks). However, concentrations of auxins-like IBA (0–2.0 mg/L) were ineffective to form reproductive buds. Subculture duration, photoperiod, and carbon source type do have influence on the in vitro flowering. The mature purple flowers were observed when the cultures were maintained in the same medium. This is the very first report that describes in vitro flowering system to overcome problems associated with flower growth and development as well as lay foundation for fruit and seed production in vitro in Swertia chirayita.

Penggunaan Adenin Sulfat Pada Perbanyakan Mikro Talas Jepang

“…Efek dari adenin sulfat dalam multiplikasi tunas juga telah ditunjukkan pada beberapa spesies tanaman, seperti Pterocarpus marsupium [21], Cichorium intybus [22], Hildegardia populifolia [23] , Stevia rebaudiana [24] , Swertia chirayita [25] dan lain-lain.…”

Plant Tissue Culture

“…In vitro flowering of valuable medicinal plants is divided on the basis of its available floral organs as follows. Whereas other factors such as optimum temperature, relative humidity, and nature of carbon source also play important roles in the formation of reproductive buds [52]. Flower cultures have not been a subject of large-scale experimentation.…”

Comparative Study of in Vitro and in Vivo Inflorescence of Feathered Amaranth

“…Globally the floriculture market has been estimated to be worth about US $60 billion [1]. Flower formation in whole plants grown in the glasshouse or under field conditions (herewith referred to as the in vivo conditions) are under the complex control of many factors [2]. In vitro flowering may be useful to aid gaining a better understanding of regulation of flowering as this phenomenon could occur in many different plants under highly controlled in vitro environmental conditions [3]- [7].…”

“…Studies on in vitro flowering are not only of academic interest but also of applied interest [2]. For example, in vitro flowering could be used to aid plant breeding [8].…”

“…Pollen morphology and germination from in vitro and in vivo florets of feathered amaranth, PD = pollen diameter; PL = pollen tube length, PG = pollen germination means of 50 replications ± SD 2. Values are means of 25 replications ± SD.…”