In VitroFlowering and Micropropagation ofHyptis suaveolens(Linn.) Poit.—An Important Medicinal Herb

Abstract

An optimized protocol for micropropagation of Hyptis suaveolens was developed. A 100% shoot regeneration frequency and 16.9 shoots per node explant were achieved on Murashige and Skoog medium (MS) containing 1.0 mg.L −1 N6-benzylaminopurine (BAP) and 0.5 mg.L −1 gibberellic acid (GA 3 ). Node explants of microshoots could be induced for proliferation of a large number of harvestable shoots on the same medium. The shoots developed in vitro inflorescences and 8.5 flowers per shoot on MS medium supplemented with 2.0 mg.L −1 indole-3-butyric acid (IBA). Rooting of microshoots was induced on MS medium containing 1.0 mg.L −1 indole-3-acetic acid (IAA). The plantlets were acclimatized, transplanted to natural soil, and evaluated. The regeneration protocol may provide a means of ex situ germplasm conservation and for research of active constituents.

Efficient Plant Regeneration from Explants of Compact Oregano (<i>Origanum compactum</i> Bentham)

“…The effect of GA 3 alone or in combination with cytokinins or auxins have been explored in other Lamiaceae species, such as the study by Mandal (2013) [55] which showed that the addition of 4.44 µM BAP and 1.44 µM GA 3 to the culture medium is favorable for the multiplication of Hyptis suaveolens and allows a total regeneration of the explants. In addition, Samantaray et al ( 2013) [56] obtained a maximum regeneration rate of Vitex trifolia explants in the medium containing 1.14 µM GA 3 , 0.54 µM NAA, and 271.5 µM Adenine.…”