<i>In vitro</i> genotoxicity of root canal sealers

Brzović, Valentina; Miletić, Ivana; Želježić, Davor; Mladinić, Marin; Kašuba, Vilena; Ramić, Snježana; Anić, Ivica

doi:10.1111/j.1365-2591.2008.01510.x

Cited by 32 publications

(31 citation statements)

References 45 publications

(78 reference statements)

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“…In a further in vitro study GuttaFlow was tested on human peripheral blood lymphocytes using the comet assay and chromosomal aberration analysis. The results of this study displayed a good biocompatibility and genotoxicity of GuttaFlow [16]. …”

Section: Introductionmentioning

confidence: 96%

Tissue reaction to sealing materials: different view at biocompatibility

et al. 2010

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The biodegradability of root canal sealers in areas other than the root canal system is crucial to the overall success rate of endodontic treatment. The aim of the present study was to investigate, the cell and tissue reaction to GuttaFlow and AHPlus, both in vitro and in vivo. For the in vitro experiments the materials were incubated with Human Periodontal Ligament Fibroblasts and cell proliferation and cytotoxicity analyses were performed. Additional fluorescence-microscope stainings were carried out in order to visualize cell growth and morphology. For assessment of the tissue reaction to the materials a subcutaneous implantation model in Wistar rats was employed and the inflammatory response to the materials was visualized by means of general and specific histology after 6 weeks. Human gingival fibroblasts proliferation seemed to be dependent upon dental material and cultivation time. After an incubation period of 96 hrs AHPlus proved to be significantly (p < 0.002) more cytotoxic than GuttaFlow, as only a small number of fibroblasts survived on AHPlus. In vivo, GuttaFlow was surrounded by a fibrous capsule and no degradation took place, while AHPlus induced a well-vascularized granulation tissue in which the material was phagocyted by macrophages. The results of this study demonstrate that a potential cytotoxic effect of a sealing material may beneficial in order to have antibacterial properties and induce self degradation when accidentally extruded over the apical foramen.

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Section: Introductionmentioning

confidence: 96%

Tissue reaction to sealing materials: different view at biocompatibility

et al. 2010

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“…Several studies were performed to demonstrate that not only MTA but also its basic component PC presents excellent biological properties (6,7). Both materials were able to promote cellular response favorable to bone and dental tissue repair, including enhancement of important osteogenic factors gene expression, such as bone morphogenetic proteins and collagenous proteins synthesis (8)(9)(10). A drawback of PC is its lack of radiopacity.…”

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confidence: 99%

Cytotoxicity of Portland Cement with Different Radiopacifying Agents: A Cell Death Study

Cornélio

Salles

Paz

et al. 2011

Journal of Endodontics

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“…It is also widely mentioned in endodontic literature that the cytotoxicity of GuttaFlow is less than GP or other root canal sealers [40][41][42] , and that it has acceptable biocompatibility in terms of genotoxicity 43) . Therefore, whether the extruded GuttaFlow might pose a potential damage to the periapical tissue would require further studies to clarify and confirm.…”

Section: Discussionmentioning

confidence: 99%