<i>In vitro</i>gossypol induced spermatozoa motility alterations in rabbits

Roychoudhury, Shubhadeep; Massányi, Peter; Slamečka, J.; Chlebec, Ivan; Trandžík, J.; Bulla, J.; Okab, Aly B.; Taha, Taha A.; Salem, M. H.; Ayoub, Mohammed Akli

doi:10.1080/03601230903163905

Cited by 10 publications

(4 citation statements)

References 47 publications

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“…2014, Rafay andParkanyi 2016). Some herbal extracts have been proven to have effects on male infertility, for example, gossypol, papaya seed, neem oil, neem seaweed and verbascoside (Mosher and Pratt 1991, Dehghan et al 2005, Roychoudhury et al 2009, Okab et al 2013, Vizzarri et al 2019.…”

Section: Discussionmentioning

confidence: 99%

Time and Dose-Dependent Effects of Viscum Album Quercus on Rabbit Spermatozoa Motility and Viability in Vitro

Halo

Massányi²,

Greń³

et al. 2019

Physiol Res

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The target of this study was to evaluate the effect of extract of the European mistletoe -Viscum album quercus L. on spermatozoa motility and viability in vitro. The CASA system was used to determine the spermatozoa motility parameters at different time intervals (0, 1, 2 and 3 h) and spermatozoa viability was determined in five different doses of Viscum album quercus L [10 (QA), 6.6 (QB), 3.3 (QC), 2.5 (QD) and 2 (QE) mg/ml]. Results in experimental groups detected a significant deterioration on rabbit spermatozoa after 1, 2 and 3 hours, compared to the control. The initial total spermatozoa motility showed increased value for all doses of Viscum album quercus in comparison to control. After in vitro culture a dose-dependent decrease (QA: reduction of 69.7 %, QB: reduction of 40.9 %) was found. For the progressive spermatozoa most significant decrease (86.8 % for QA vs. 48.5 % for QB) was detected compared to the control after 3 hours of culture. Spermatozoa viability (MTT test) was decreased in all experiment groups at the end of experiment, but the differences were not significant.Significant alterations of membrane integrity were found in groups with the highest Viscum album quercus concentration (QA, QB), but acrosome integrity showed no significant changes. Results suggest negative dose-and time-dependent effect ofViscum album quercus at higher doses on spermatozoa motility and viability parameters in vitro.

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Section: Discussionmentioning

confidence: 99%

Time and Dose-Dependent Effects of Viscum Album Quercus on Rabbit Spermatozoa Motility and Viability in Vitro

Halo

Massányi²,

Greń³

et al. 2019

Physiol Res

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“…The accuracy of CASA for evaluation of rabbit spermatozoa motility, as well as Makler chamber manual examination has already been described. [41,43,45] A heated stage (37 • C) was used during the entire analysis and multiple fields in less than 2 seconds/field were analysed for each sample as per the specific CASA -Sperm Vision software for rabbit spermatozoa motility analysis.…”

Section: Spermatozoa Motilitymentioning

confidence: 99%

Cadmium toxicity at low concentration on rabbit spermatozoa motility, morphology and membrane integrityin vitro

Roychoudhury

Massányi

Bulla

et al. 2010

Journal of Environmental Science and Health, Part A

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In this study the effect of cadmium on various parameters of spermatozoa motility, morphology as well as on the spermatozoa membrane integrity in rabbits was analyzed in vitro, experimental concentrations ranging from 0.62 to 0.98 micro g CdCl(2)/mL. Pooled rabbit (n = 5) semen was cultured in vitro with cadmium and subsequently diluted to various experimental concentrations apart from control which received no cadmium exposure. Using computer assisted semen analysis method (CASA) we detected decrease of total motility with in the higher concentration range at Time 0. However, with increasing time (after 1 and 2 h of culture), cadmium exerted deleterious effect leading to significant motility reduction in comparison to control. A similar trend was exhibited in case of progressive motility, too. Most of the spermatozoa distance and velocity parameters detected no significant change in comparison to control at the beginning of culture (Time 0), although the toxic effect became significant (P < 0.05) with the passage of culture time (Times 1 and 2 h) in all concentrations. Analysis of spermatozoa morphology detected significant (P < 0.05) alterations at higher concentrations. At higher concentrations acrosomal changes, head without flagellum/separated flagellum, broken flagellum and other abnormalities were significantly higher (P < 0.05), while knob-twisted flagellum and small heads differed significantly (P < 0.05) in comparison to control at all concentrations. In regards to flagellum torso, flagellum ball and retention of cytoplasmic drop statistically higher values (P < 0.05) were noted at the maxium experimental concentration only. Annexin analysis for detection of spermatozoa with disordered membranes revealed higher occurrence of positive spermatozoa in cadmium exposed groups. Annexin-positive reactions suggested alterations in anterior part of head (acrosome) and in flagellum (mitochondrial segment) of spermatozoa. This paper underlines that cadmium is highly toxic for rabbit spermatozoa, as visualized by the toxic effects on parameters of spermatozoa motility, morphology and membrane integrity. The toxic effect is more drastic at higher concentrations. This study also indicates that cadmium requires a minimum one hour incubation time to exert its deletorious effects on various parameters of spermatozoa, particularly at low concentrations.

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“…It may be that the residual gossypol in LCSM exerted an inhibitory effect on liver glycolysis. In vitro studies found that decreased sperm motility of rabbit could result from a reduced energy supply after inhibition of glycolysis by gossypol [ 97 ]. In addition, gossypol is thought to inhibit glycolysis by inhibiting lactate dehydrogenase isozyme 5 [ 98 ].…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic Analysis of Liver in Silver sillago, Sillago sihama Fed with High-Level Low-Gossypol Cottonseed Meal in Replacement of Fishmeal Diet

Líu

Zhou

Dong

et al. 2023

Animals

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Understanding the molecular mechanisms involved in adaptation to alternate diets has become a significant concern, as increasing amounts of fishmeal (FM) protein in aquafeeds are being substituted with plant protein. Thus, the goal of this study was to assess growth performance, quality, and liver function of juvenile Sillago sihama (S. sihama) through growth indices, whole-body composition, histology of the liver, and RNA-sequencing (RNA-seq), after they were fed a formulated diet with 64% low-gossypol cottonseed meal (LCSM) for 56 days, compared to those fed a traditional FM-based diet. Indicators of growth, including final body weight (FBW), weight gain rate (WGR), specific growth rate (SGR), protein efficiency ratio (PER), and condition factor (CF), were considerably lower in the 64% LCSM (R64) group than in the FM diet group. In the R64 diet, the whole crude lipid was significantly lower than in the FM diet. The hematoxylin–eosin section showed that dietary high levels of LCSM resulted in diffuse lipid vacuolation in the liver of S. sihama. According to a liver transcriptome analysis, high LCSM intake in the diet significantly impacted lipid synthesis and catabolism, elevated pathways for cholesterol synthesis, blocked several amino acid metabolic pathways, and adversely affected hepatic gluconeogenesis and glycolysis. The findings of this study indicate that feeding high levels of LCSM in S. sihama is harmful to the growth of the organism and can harm the liver’s structural integrity, as well as obstruct the normal metabolism of amino acids, lipids, and carbohydrates. Therefore, it is not recommended to substitute LCSM for high levels of FM in the diet of S. sihama.

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In vitrogossypol induced spermatozoa motility alterations in rabbits

Cited by 10 publications

References 47 publications

Time and Dose-Dependent Effects of Viscum Album Quercus on Rabbit Spermatozoa Motility and Viability in Vitro

Time and Dose-Dependent Effects of Viscum Album Quercus on Rabbit Spermatozoa Motility and Viability in Vitro

Cadmium toxicity at low concentration on rabbit spermatozoa motility, morphology and membrane integrityin vitro

Transcriptomic Analysis of Liver in Silver sillago, Sillago sihama Fed with High-Level Low-Gossypol Cottonseed Meal in Replacement of Fishmeal Diet

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