<i>In vitro</i> growth inhibition and cytotoxicity of <i>Euphorbia caducifolia</i> against four human cancer cell lines and its phytochemical characterisation

Bano, Shaheen; Siddiqui, Bina Shaheen; Farooq, Ahsana Dar; Begum, Sabira; Siddiqui, Faheema; Kashif, Muhammad; Azhar, Mudassar

doi:10.1080/14786419.2017.1305380

Cited by 12 publications

(5 citation statements)

References 17 publications

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“…The second focus of this study was to analyse the potency of MP in reducing in vivo toxicity of Sorafenib using a promising zebra fish model 59 . The leaf extracts of different plants having MP as main constituent exhibited cytotoxicity, anti‐angiogenesis, anti‐proliferative and apoptosis induction activities on many human cancer cells 40,60‐63 . An in vivo study has proven the hepato‐protective property of MP without causing toxic effects 45 .…”

Section: Discussionmentioning

confidence: 99%

“…59 The leaf extracts of different plants having MP as main constituent exhibited cytotoxicity, anti-angiogenesis, anti-proliferative and apoptosis induction activities on many human cancer cells. 40,[60][61][62][63] An in vivo study has proven the hepato-protective property of MP without causing toxic effects. 45 From our study results, we conclude that the 3 mmol/L of MP used in this study is a safer dose as the higher tested concentration (5 nmol/L) did not show toxicity to the embryos.…”

Section: Discussionmentioning

confidence: 99%

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Methyl Palmitate—A suitable adjuvant for Sorafenib therapy to reduce in vivo toxicity and to enhance anti‐cancer effects on hepatocellular carcinoma cells

Breeta

Grace

Wilson

2020

Basic Clin Pharma Tox

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This study focused on evaluating the potency of Methyl Palmitate in reducing in vivo toxicity with enhancement of anti‐cancer effects of Sorafenib. In vitro anti‐cancer effects on human Hep‐G2 cell line were analysed by MTT, Trypan blue, clonogenic, wound scratch migration and TUNEL assays. An in vivo study for anti‐angiogenesis effect, toxicity and teratogenicity was analysed in Zebrafish embryos. The combination of Sorafenib (4.5 µmol/L) with Methyl Palmitate (3 mmol/L) significantly enhanced anti‐cancer effects on Hep‐G2 cell line by increasing cytotoxicity (P ≤ .05 in MTT assay; P ≤ .01 in Trypan blue assay), apoptosis (P ≤ .05) and decreasing the metastatic migration (P ≤ .01) than Sorafenib alone treatment. A prominent inhibition of angiogenesis in vivo was observed for combination treatment. At 5 dpf, only <20% toxicity was observed for 3 mmol/L Methyl palmitate while it was 65.75% for Sorafenib treatment which implies that it is a safer dose for in vivo treatments. A highly significant (P ≤ .001) reduction (43.20%) in toxicity was observed in combination treatment. Thus, the Sorafenib‐Methyl Palmitate combination showed a promising treatment effect with significantly reduced in vivo toxicity when compared with Sorafenib alone treatment, and hence the Methyl Palmitate may serve as a good adjuvant for Sorafenib therapy.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Methyl Palmitate—A suitable adjuvant for Sorafenib therapy to reduce in vivo toxicity and to enhance anti‐cancer effects on hepatocellular carcinoma cells

Breeta

Grace

Wilson

2020

Basic Clin Pharma Tox

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“…Modern medicine and traditional Chinese medicine studies have found that Euphorbia fischeriana displays anti-tumor effects and is clinically used to treat lung cancer and malignant tumors of the digestive system. Various monomer compounds extracted from Euphorbia fischeriana have demonstrated good therapeutic effects on various malignant tumors and leukemia, and have no long-term adverse reactions, unlike chemotherapy ( Choene & Motadi, 2016 ; Bano et al, 2017 ). The root of Euphorbia contains diterpenoids, triterpenoids, flavonoids, and volatile oils.…”

Section: Introductionmentioning

confidence: 99%

WASH regulates the oxidative stress Nrf2/ARE pathway to inhibit proliferation and promote apoptosis of HeLa cells under the action of Jolkinolide B

Hong

Liu

Kong

et al. 2022

PeerJ

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Jolkinolide B (JB), a diterpenoid compound isolated from the roots of Euphorbia fischeriana, has gained research attention for its antitumor effects. In recent years, JB reportedly displayed anti-tumor activity in solid tumors, such as breast, ovarian, and prostate cancer, and leukemia. In this study, we evaluated the effect of JB on HeLa cells with a focus on cell growth inhibition and related mechanisms. HeLa cells were cultured in vitro and divided into a blank control group, HeLa-Scramble (0, 0.25, 0.5 mM), and Wiskott-Aldrich syndrome protein and SCAR homolog (WASH) protein silenced group, HeLa-shWASH (0, 0.25, 0.5 mM). Morphological changes were observed using an inverted microscope. The inhibition rate of cell proliferation was detected using the WST-1 method. Flow cytometry Brdu+PI double standard method was used to detect cell replication ability and FITC+PI double standard method was used to detect cell apoptosis rate. Western blot was used to verify the expression of Nrf2, HO-1, WASH, Bax, Bcl-2, and PCNA. The mRNA expression of cytokines (IL-1α, IL-6, and IL-8) was detected using RT-qPCR. The results showed that JB induced cell apoptosis and arrested cells at the G2/M phase in HeLa-shWASH cells compared with HeLa-Scramble cells (P < 0.05, P < 0.01, respectively). In addition, JB upregulated IL-1α, IL-6, and IL-8 in HeLa-shWASH cells. We conclude that WASH protein participates in JB-induced regulation of the Nrf2/ARE pathway, aggravates inflammatory responses, and promotes cancer cell apoptosis, thus inhibiting the proliferation and invasion abilities of HeLa cells. JB may have anti-tumor effects and potential clinical value for the treatment of cervical cancer.

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“…Various monomer compounds extracted from Euphorbia fischeriana have demonstrated good therapeutic effects on various malignant tumors and leukemia, and have no long-term adverse reactions, unlike chemotherapy. (4)(5) The root of Euphorbia contains diterpenoids, triterpenoids, flavonoids, and volatile oils. Jolkinolide B (JB) is a diterpenoid extracted from the root of Euphorbia; the compound has multiple biological activities (such as anti-proliferation, anti-bacterial, and anti-viral), (6) a molecular formula of C 20 H 26 O 4 , and a molecular weight of 330.42 Da.…”

Section: Introductionmentioning

confidence: 99%

Peer Review #2 of "WASH regulates the oxidative stress Nrf2/ARE pathway to inhibit proliferation and promote apoptosis of HeLa cells under the action of Jolkinolide B (v0.2)"

2022

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In vitro growth inhibition and cytotoxicity of Euphorbia caducifolia against four human cancer cell lines and its phytochemical characterisation

Cited by 12 publications

References 17 publications

Methyl Palmitate—A suitable adjuvant for Sorafenib therapy to reduce in vivo toxicity and to enhance anti‐cancer effects on hepatocellular carcinoma cells

Methyl Palmitate—A suitable adjuvant for Sorafenib therapy to reduce in vivo toxicity and to enhance anti‐cancer effects on hepatocellular carcinoma cells

WASH regulates the oxidative stress Nrf2/ARE pathway to inhibit proliferation and promote apoptosis of HeLa cells under the action of Jolkinolide B

Peer Review #2 of "WASH regulates the oxidative stress Nrf2/ARE pathway to inhibit proliferation and promote apoptosis of HeLa cells under the action of Jolkinolide B (v0.2)"

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