<i>In Vitro</i>Immunomodulatory Effects of Ten Commonly Used Herbs on Murine Lymphocytes

Wilasrusmee, Chumpon; Kittur, Smita; Siddiqui, Josephine; Bruch, David; Wilasrusmee, Skuntala; Kittur, Dilip S.

doi:10.1089/107555302760253667

Cited by 81 publications

(64 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The second study 15 demonstrated that macrophage activity increased 10-fold in vivo after intravenous (IV) injection of the purified polysaccharide. An in vitro study 4 on human lymphocytes confirmed that E purpurea had no lymphoproliferative effect and showed no effect on lymphocyte production of IL-2 or IL-10. Burger et al 16 first reported use of an unpurified extract of E purpurea in human macrophages, which increased production of IL-1, IL-10, and TNF-␣ to levels comparable to that achieved with LPSs.…”

Section: Casementioning

confidence: 90%

Activation of Autoimmunity Following Use of Immunostimulatory Herbal Supplements

Lee¹,

Werth²

2004

Arch Dermatol

104

View full text Add to dashboard Cite

Background: Evidence for the scientific basis of purported therapeutic effects and adverse effects of herbal supplements continues to grow. Many herbal supplements are touted for their immunostimulatory properties, and both in vitro and in vivo experiments have supported this claim. Although this explains their beneficial effects in preventing or curtailing disease, to our knowledge, no immunostimulatory herbal supplements have been reported to exacerbate disorders of immune system overactivity.

show abstract

Section: Casementioning

confidence: 90%

Activation of Autoimmunity Following Use of Immunostimulatory Herbal Supplements

Lee¹,

Werth²

2004

Arch Dermatol

104

View full text Add to dashboard Cite

show abstract

“…The cell culture was performed in microtiter plates (TTP, Techno Plastic Products, Trasadingen, Switzerland) with incubation of the cells (50,000 cells/mL) and methanol extracts (10, 50, 100 and 200 µg/mL) in the presence or absence of PHA (Sigma, 5 µg/mL) for 72 hours at 37 °C and in 5% CO 2 (Jouan, model IG150, Saint Herbain, France) (Yasni et al, 1993;Roseghini et al, 2006). The cell and mitogen concentrations were determined by the growth curve (data not shown), while the concentrations of the extracts were defined according to the literature (Wilasrusmee et al, 2002;Manosroi, Saraphanchotiwitthaya, Manosroi, 2003;Mehrotra et al, 2003). The assay revelation was performed by blue tetrazolium reduction assay (MTT), with the addition of 10 µL of MTT at 5 mg/mL (Amreco, Solon, Ohio, USA) in 0.9% NaCl to each well of the microplate three hours before the end of the incubation period.…”

Section: Cell Culturementioning

confidence: 99%

Evaluation of the proliferative activity of methanol extracts from six medicinal plants in murine spleen cells

Zandonai

Coelho

Ferreira

et al. 2010

Braz. J. Pharm. Sci.

View full text Add to dashboard Cite

A number of natural compounds have been used as immunomodulatory agents, enabling the function of the immune system to be modified by stimulating or suppressing it. There has been increasing interest in the study of therapeutic action of plant extracts regarding their immunomodulatory activity. The aim of this study was to identify and evaluate the action of extracts of the medicinal plants Calophyllum brasiliense, Ipomoea pes-caprae, Matayba elaeagnoides, Maytenus robusta, Rubus imperialis and Vernonia scorpioides on the development of spleen cells from mice, using the in vitro cellular proliferation assay. The cells, obtained by mechanical rupture of mice spleen (5x10 4 cells/mL), were incubated with methanol extracts (10, 50, 100 and 200 mg/mL) and phytohemagglutinin (PHA, 5 mg/mL). The basal control for proliferation consisted of cells alone, while the positive control consisted of cells and PHA. The cell culture was kept at 37 o C in 5% CO 2 for 72 hours, and cell proliferation was revealed by the blue tetrazolium reduction assay (MTT). The results were expressed as percentage of growth and were analyzed using the Kruskal-Wallis and Mann-Whitney tests. The C. brasiliense, I. pes-caprae and M. elaeagnoides extracts showed dose-dependent induction of cell proliferation, with a significant increase in cell proliferation (p<0.03) and percentage growth of 88.2%, 73.1% and 52.7%, respectively, suggesting T lymphocyte stimulation. By contrast, M. robusta, R. imperialis and V. scorpioides extracts showed significance only with a negative percentage of growth, suggesting inhibition of cell proliferation (p<0.04). Further biomonitoring studies will enable the fractions and isolated substances responsible for the immunomodulatory activities to be identified. Várias substâncias de origem natural têm sido utilizadas como agentes imunomoduladores, permitindo modificar a função do sistema imune e propiciando o estudo de atividades terapêuticas de extratos de plantas. Este trabalho objetivou identificar a atividade imunomodulatória dos extratos de seis plantas medicinais da flora brasileira, Calophyllum brasiliense, Ipomoea pes-caprae, Matayba elaeagnoides, Maytenus robusta, Rubus imperialis e Vernonia scorpioides, sobre a proliferação de células esplênicas de camundongos. As células esplênicas murinas obtidas por ruptura mecânica do baço (5x14 3 células/mL) foram incubadas com os extratos metanólicos das plantas (10, 50, 100, 200 mg/mL) e fito-hemaglutinina (PHA, 5 mg/mL). O controle basal de proliferação foi constituído de células apenas e o controle positivo formado por células e PHA. O cultivo celular foi mantido a 37 o C, 5% de CO 2 , 72 horas, com quantificação da proliferação celular pelo ensaio de redução do azul de tetrazólio. Os resultados expressos em percentagem de crescimento foram analisados pelos testes de Kruskal-Wallis e Mann-Whitney. Os extratos de C. brasiliense, I. pes-caprae e M. elaeagnoides mostraram indução dose-dependente da proliferação celular (p<0,03), com percentagem de crescimento de, respecti...

show abstract

“…Modulatory effects of ginger on in vitro and in vivo cellular and humoral immune response was observed in mice and fish [12][13][14][15]. 6-Gingerol, a natural component of ginger, has been reported to possess antiinflammatory and antitumorigenic activities.…”

Section: Introductionmentioning

confidence: 99%

Experimental immunology The effect of multi-component herbal remedy PERVIVO on cellular immunity and tumor angiogenesis in mice

Bałan¹,

Stankiewicz²,

Skopińska-Różewska³

et al. 2013

cejoi

View full text Add to dashboard Cite

show abstract

In VitroImmunomodulatory Effects of Ten Commonly Used Herbs on Murine Lymphocytes

Abstract: Our study provides a uniform survey of the immunomodulatory properties of 10 commonly used herbal products and paves the way for testing these effects in vivo and in clinical setting.

Cited by 81 publications

References 35 publications

Activation of Autoimmunity Following Use of Immunostimulatory Herbal Supplements

Activation of Autoimmunity Following Use of Immunostimulatory Herbal Supplements

Evaluation of the proliferative activity of methanol extracts from six medicinal plants in murine spleen cells

Experimental immunology The effect of multi-component herbal remedy PERVIVO on cellular immunity and tumor angiogenesis in mice

Contact Info

Product

Resources

About