In Vitro Inhibition of Biofilm Formation by Staphylococcus Aureus Under the Action of Selected Plant Extracts

Abstract

In our study we investigated the ability of selected plant extracts to inhibit the formation of biofilms produced by Staphylococcus aureus. In the first phase, we focused on the optimisation of conditions for the correct method of an approach. For optimisation, we standardized the culture media and the bacterial culture in order to obtain interpretable results. The TSB (Tryptone Soya Broth) medium was used for the preparation of an inoculum from the bacterial suspension. For the in vitro tests of antibiofilm activity against the species Staphylococcus aureus CCM 3953, we used propylene glycol (PG) plant extracts from sage and rosemary, prepared in three different concentrations of 0.01 %, 0.05 % and 0.1 %. The tests were implemented in microtitre plates using crystal violet dye at 0.1 % concentration for visualization of the intensity of a biofilm. The results were obtained, by spectrophotometric measurements at a wavelength of 550 nm. Both rosemary and sage plant extracts had a significant effect on the formation of a biofilm by S. aureus. The antibiofilm activity was concentration-dependent as the formation of biofilm was reduced more effectively with increasing concentration of the extracts. The best antibiofilm activity was observed with 0.1 % rosemary extract resulting in 94 % inhibition of the biofilm formation.

Antimicrobial, antibiofilm and biochemichal properties of Thymus vulgaris essential oil against clinical isolates of opportunistic infections

“…The established antibiofilm-forming antimicrobial activity of thyme essential oil is of great importance (Huma et al, 2014;Piegerová et al, 2019), for mouth cavity microorganisms are known to exist mostly in the form of biofilm, which causes a significant problem for treatment of inflammatory diseases of the mouth cavity. The determined antibiofilmforming ability of Th.…”

Targeting S. epidermidis Biofilms by the Tetracycline-Loaded Nanogel Surface Functionalized with Savinase, DNase, and Cellulase

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Nanotechnologies for control of pathogenic microbial biofilms

“…9,90 This is recognized as a huge burden to the public healthcare sector, food processing industry, desalination plants, and drinking water distribution sector, and requires urgent attention. The search for new antimicrobial agents, 1,2 ultrasound, 13,81 physical therapy such as photodynamic, 14,88,89 and bioelectrochemical methods 17,18 with antimicrobial agents and potency enhancement of existing antimicrobial agents are some of the ways that are currently being explored to control pathogenic biofilm infections.…”

“…1 Biofilm formation occurs wherever there are nutrients, moisture and interfaces. 1,2 In the environment, biofilms are formed by bacteria, algae, fungi, and protozoa. Generally, biofilm formation depends on a combination of genetic and environmental factors.…”