In vitro interaction between Phlebiopsis sp. and Ganoderma philippii isolates

Abstract

Ganoderma philippii causes root-rot disease in a wide range of commercial perennial woody crops such as rubber and tropical acacias. In terms of productivity loss, the disease is considered as the most economically damaging disease of Acacia mangium. Many strategies to manage root rot have been developed and applied, including application of biological control which has focused on species and strains of the ascomycete genus Trichoderma. This research aimed to test the potential of a wood-rotting basidiomycete as a biocontrol agent for root rot caused by Ganoderma philippii. This basidiomycete was isolated from a Eucalyptus pellita stump in a coppicing trial in Riau province, central Sumatra. DNA sequence analysis indicated that this isolate is most likely a Phlebiopsis sp. In vitro tests and microscopic morphological description of mycelial interactions between the Phlebiopsis and G. philippii isolates showed a mycoparasitic reaction. This result offered an alternative potential fungal isolate for root-rot management in Indonesia as Phlebiopsis gigantea is already successfully used as a biocontrol agent for managing root rot caused by Heterobasidion annosum throughout pine forests in Europe and North America.

Acacia plantations in Indonesia facilitate clonal spread of the root pathogen Ganoderma philippii

“…philippii–A . mangium pathosystem (Agustini et al , ). This may be an effective strategy to prevent new infections initiated by basidiospores in disease‐free areas, but, given the propensity for clonal spread, is not expected to eradicate the pathogen from plantations with pre‐existing inoculum.…”

Mycoparasitic Scytalidium parasiticum as a potential biocontrol agent against Ganoderma boninense basal stem rot in oil palm

“…Mycoparasitic Phlebiopsis sp. was found to inhibit, overgrow, and coil around G. philippii (Agustini et al 2014). The results of our studies suggested that S. parasiticum was not a biotrophic mycoparasite but a potential necrotrophic hyperparasite.…”

“…Cladobotryum semicirculare and S. ganodermophthorum also reduced the production of commercially medicinal G. lingzhi and G. lucidum fruiting bodies in Taiwan and Korea, respectively (Kang et al 2010;Kirschner et al 2007). In a study on root rot of Eucalyptus pellita, a Phlebiopsis species was found to parasitize and reduce the viability of G. philippii (Agustini et al 2014). This Phlebiopsis species has been proposed as a potential biocontrol agent against G. philippii.…”

Pengujian Penanda Jenis Spesifik Pada Jamur Yang Berpotensi Sebagai Agens Pengendali Hayati Penyakit Busuk Akar Pada Akasia

“…Terdapat beberapa jenis jamur dengan track record yang bagus sebagai pengendali hayati sebelumnya dari koleksi isolat yang dimiliki dari hasil isolasi sampel di lapangan, antara lain jamur Phlebiopsis sp. 1 (Agustini, Wahyuno, Indrayadi, & Glen, 2014), jamur Cerrena sp. dan jamur Phlebia spp (Agustini, Francis, Glen, Indrayadi, & Mohammed, 2014).…”