<i>In Vitro</i>. Investigation of the Protective Effects of Tannic Acid Against the Activities of<i>Naja kaouthia</i>. Venom

Pithayanukul, Pimolpan; Ruenraroengsak, Pakatip; Bavovada, Rapepol; Pakmanee, Narumol; Suttisri, Rutt

doi:10.1080/13880200601112885

Cited by 9 publications

(8 citation statements)

References 20 publications

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“…When tannins are present [HA(+)] the extract is able to neutralize the neuromuscular blockade promoted by Bjssu venom (n = 4). The protective effect of commercially available tannic acid was already demonstrated in vivo after subcutaneous injection in mice envenomed with Crotalus adamanteus venom (decreased hemorrhage, creatine kinase release and lethal activity) [ 27 ], or after its pre-incubation for 1 h at 37 ºC with Naja kaouthia Lesson (Elapidae) venom before intravenous injection in mice [ 28 ].…”

Section: Resultsmentioning

confidence: 99%

“…In regard to this issue, tannins and tannic acid share identical properties since both promote the precipitation of proteins [ 29 ]. Tannic acid has been already proved to precipitate proteins present in several snake venoms including venoms of the Elapidae family ( Naja kaouthia ) [ 28 ] and the Viperidae family ( C.d. terrrificus, Bothrops jararacussu ) [ 16 ] and Crotalus adamanteus [ 27 ].…”

Section: Resultsmentioning

confidence: 99%

“…The literature clearly shows the effectiveness of tannic acid in decreasing the venom toxicity of some Viperidae species such as C. d. terrificus [ 16 ], C. adamanteus [ 27 ] and B. jararacussu [ 16 ], and Elapidae, i.e., N. kaouthia [ 28 ] snake venoms, likely by precipitating venom proteins [ 16 ] and hence promoting its denaturation and loss of toxicity. Like tannic acid, tannins from three different plant species demonstrated to be a crucial antiophidian compound present in ME extract, and in HA extract [ 16 ].…”

Section: Resultsmentioning

confidence: 99%

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In Vitro Antiophidian Properties of Dipteryx alata Vogel Bark Extracts

Rubem-Mauro

et al. 2010

Molecules

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Extracts from Dipteryx alata bark obtained with different solvents (hexane, dichloromethane, ethyl acetate and methanol) were mixed in vitro with Bothrops jararacussu (Bjssu, 40 μg/mL) and Crotalus durissus terrificus (Cdt, 15 μg/mL) snake venoms, and applied to a mouse phrenic nerve-diaphragm preparation to evaluate the possible neutralization of venom effects. Cdt venom neurotoxic effect was not inhibited by any of the extracts, while the neurotoxic and myotoxic actions of Bjssu venom were decreased by the methanolic extract. This inhibition appears to be augmented by tannins. Dichloromethane bark extract inhibited ~40% of Bjssu venom effects and delayed blockade induced by Cdt. The methodology used to determine which extract was active allows inferring that: (i) phenolic acids and flavonoids contained in the methanolic extract plus tannins were responsible mostly for neutralization of Bjssu effects; (ii) terpenoids from the dichloromethane extract may participate in the anti-Cdt and anti-Bjssu venom effects; (iii) a given extract could not inhibit venoms from different species even if those belong to the same family, so it is improper to generalize a certain plant as antiophidian; (iv) different polarity extracts do not present the same inhibitory capability, thus demonstrating the need for characterizing both venom pharmacology and the phytochemistry of medicinal plant compounds.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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In Vitro Antiophidian Properties of Dipteryx alata Vogel Bark Extracts

Rubem-Mauro

et al. 2010

Molecules

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“…In vitro treatment showed that tannic acid caused dose-dependent AChE inhibition significantly, such that 0.04 mM of tannic acid reduced the AChE activity to 37% of the control in the nervous tissue of L. acuminata [ 92 ]. In addition, another in vivo research on AChE activity showed that tannic acid completely neutralized the AChE activity in Naja kaouthia venom [ 96 ].…”

Section: Terminalia Chebula Retziusmentioning

confidence: 99%

A Review on Potential Mechanisms ofTerminalia chebulain Alzheimer’s Disease

Afshari

Sadeghnia

Mollazadeh

2016

Advances in Pharmacological Sciences

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The current management of Alzheimer's disease (AD) focuses on acetylcholinesterase inhibitors (AChEIs) and NMDA receptor antagonists, although outcomes are not completely favorable. Hence, novel agents found in herbal plants are gaining attention as possible therapeutic alternatives. The Terminalia chebula (Family: Combretaceae) is a medicinal plant with a wide spectrum of medicinal properties and is reported to contain various biochemicals such as hydrolysable tannins, phenolic compounds, and flavonoids, so it may prove to be a good therapeutic alternative. In this research, we reviewed published scientific literature found in various databases: PubMed, Science Direct, Scopus, Web of Science, Scirus, and Google Scholar, with the keywords: T. chebula, AD, neuroprotection, medicinal plant, antioxidant, ellagitannin, gallotannin, gallic acid, chebulagic acid, and chebulinic acid. This review shows that T. chebula extracts and its constituents have AChEI and antioxidant and anti-inflammatory effects, all of which are currently relevant to the treatment of Alzheimer's disease.

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“…Pithayanukul et al [6], reported in vitro investigation of the protective effects of tannic acid against the action of Naja kaouthia venom. Melo et al [7] demonstrated the effectiveness of tannic acid in precipitating the constituent proteins of the complex mixture of snake venom (Bothrops jararacussu and Crotalus durissus terrificus).…”

Section: Introductionmentioning

confidence: 99%

Development and Preliminary Assessment of Hemoperfusion Cartridge with Tannic Acid for Toxic Proteins’ Precipitation: An In Vitro Model

Hanai-Yoshida¹,

Cavalcante²,

Campanholi³

et al. 2016

Recent Advances in Biology and Medicine

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Charcoal hemoperfusion (CHP) is one of the extracorporeal removal techniques that are used to remove toxins from the body. CHP generally is considered the preferred method for extracorporeal extraction of several toxins-toxins that are adsorbed by activated charcoal. Assessments of the tannic acid's protective effects on ophidian poisoning are associated with the toxic proteins' precipitation by tannic acid. The challenge in treating a snakebite lies in removing the injected poison with minimal damage to blood constituent proteins. An alternative is CHP, and this investigation proposed to develop a column for hemoperfuser cartridge, combining charcoal granules trapped between layers of polymeric material conjugated to tannic acid, using an in vitro model scaled to the Wistar rat, which can be tested in an animal model. The cartridge was evaluated using the 2 2 full factorial design, in duplicate, as a method to study the effects of granulated-charcoal size and tannic acid concentration on the hematologic profile (platelet and leukocyte counts) and biochemical profile (total serum protein and albumin dosages) of sheep blood. The results demonstrate that charcoal in hemoperfuser cartridge: (1) decreases the serum in sheep blood volume, as consequence, (2) increases the serum proteins' concentration, and (iii) exerts slight influence on albumin. The inclusion of tannic acid in hemoperfuser column precipitates some of serum proteins and albumin, decreasing their concentrations in the plasma serum. In conclusion, based on these effects we can suggest the use of 0.02 g tannic acid concentration and 8-20 mesh granulated charcoal in hemoperfuser cartridge for precipitating toxic proteins from snake venoms.

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In Vitro. Investigation of the Protective Effects of Tannic Acid Against the Activities ofNaja kaouthia. Venom

Cited by 9 publications

References 20 publications

In Vitro Antiophidian Properties of Dipteryx alata Vogel Bark Extracts

In Vitro Antiophidian Properties of Dipteryx alata Vogel Bark Extracts

A Review on Potential Mechanisms ofTerminalia chebulain Alzheimer’s Disease

Development and Preliminary Assessment of Hemoperfusion Cartridge with Tannic Acid for Toxic Proteins’ Precipitation: An In Vitro Model

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