In vitro lymphocyte reactivity to soluble tumor extracts in sinclair melanoma swine

Abstract

An antigen-stimulated active rosette assay was adapted as an in vitro assay for the detection of lymphocyte reactivity t o soluble tumor extracts. The specificity of this assay was demonstrated with lymphocytes from non-sensitized swine and swine sensitized t o either mammalian tuberculin o r DNCB-bovine serum albumin conjugates (DNCB-BSA). A n increase i n active rosette-forming cells (A-RFC) occurred only when sensitized lymphocytes were stimulated with specific sensitizing antigen. The Sinclair swine melanoma model represents a unique animal tumor system for basic studies on tumor development, growth and regression. This model is characterized by a high incidence of spontaneous tumor development, progressive tumor growth and a high incidence of tumor regression (Hook et al., 1977a, b ; Millikan et al., 1974). A recent study (Hook et al., 1979) with this animal model demonstrated that the incidence of cutaneous melanomas was increased by selective breeding. In this study, 85 % of the progeny from selected matings developed cutaneous melanomas by 1 year of age;

AUGMENTATION OF E‐ROSETTE FORMATION BY LYMPHOCYTES OF CANCER PATIENTS STIMULATED IN VITRO WITH TUMOUR EXTRACTS

“…1979), as well as with phytomitogens (Agbata and Kirkpatrick, 1979). Recent publications report that tumour-related host immunity in human breast cancer (Ramey el al.. 1979) and in melanoma of swine (Aultman and Hook, 1979) may be detected by a similar reaction employing crude TAA from tumour extracts. E-rosette augmentation (ERA) appears to be a rapid and technically simple procedure for detecting cell-mediated immunoreactivity in cancer patients.…”