<i>In vitro</i>maturation and fertilization of prepubertal and pubertal black Bengal goat oocytes

Khatun, Mahmuda; Bhuiyan, Mohammad Musharraf Uddin; Ahmed, Jalal Uddin; Haque, Aminul; Rahman, Bahanur; Shamsuddin, M.

doi:10.4142/jvs.2011.12.1.75

Cited by 16 publications

(14 citation statements)

References 55 publications

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“…LH has been reported to increase IVM rate in oocytes collected from the ovaries of adult animals in many species (Eppig, 1996;Junk et al, 2003;Phillips and Dekel, 1982;Zuelke and Brackett, 1990). Our results are similar and in agreement with another study (Khatun et al, 2010) that also reported a similar maturation rate of 60% in black Bengal goat oocytes.…”

Section: Discussionsupporting

confidence: 93%

Development of prepubertal goat oocytes after theirin vitromaturation and chemical activation

Hong¹,

Vettical²,

Wani³

2020

Zygote

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Summary Experiments were conducted to study in vitro maturation of prepubertal goat oocytes and their developmental potential after chemical activation. In Experiment 1, cumulus–oocytes complexes collected from the ovaries of prepubertal goats slaughtered at a local abattoir were matured in vitro in TCM-199-based medium supplemented with 10 µg/ml luteinizing hormone (LH) (treatment 1) or 10 µg/ml LH + 0.1 mM l-cysteine (treatment 2). In Experiment 2, mature oocytes were activated with either 5 µM ionomycin or 7% ethanol. After 18 h, some oocytes were randomly fixed and stained to evaluate their chromatin status, while others were cultured in embryo culture medium to study their further development. In Experiment 3, oocytes activated with 5 µM ionomycin were cultured for 7 days in one of the four different culture media [Charles Rosenkrans medium (CR-1), TCM-199, potassium simplex optimization medium (KSOM) and synthetic oviductal fluid (SOF)] to study their developmental potential. The maturation rate in control, treatment 1, and treatment 2 media did not differ from each other (P > 0.05). However, the lowest degeneration of oocytes was observed in treatment 3 (P < 0.05) when compared with the other two groups. The proportion of activated oocytes was higher, while non-activated oocytes were lower in ionomycin group when compared with the group activated with ethanol (P < 0.05). The proportions of oocytes cleaved were 65.7, 56.8, 61.0 and 54.4% in CR-1, TCM-199, KSOM and SOF medium, respectively, with no significant difference. However, further development of cleaved oocytes was better in KSOM followed by SOF.

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Section: Discussionsupporting

confidence: 93%

Development of prepubertal goat oocytes after theirin vitromaturation and chemical activation

Hong¹,

Vettical²,

Wani³

2020

Zygote

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“…In vitro maturation rate of goat's oocytes without vitrification is similar to that reported in Bangladesh (Khatun et al, 2011) and India (Purohit et al, 2012). Contrasting to the present finding, higher rates of in vitro maturation (71.4 to 81.4%) were reported in goat oocytes when cultured without vitrification elsewhere (Izquierdo et al, 2002;Anguita et al, 2007).…”

Section: Resultssupporting

confidence: 83%

Vitrification of immature oocytes of goats in Bangladesh

Sharif¹,

Choudhury²,

Rahman³

et al. 2019

Bangl. vet.

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Cryopreservation of oocytes and embryos by vitrification can have advantages in assisted reproductive technologies (ARTs) in mammals. The aim of this study was to establish an effective vitrification procedure and cryodevice for goat’s oocytes in Bangladesh. Cumulus oocyte complexes (COCs) were collected from ovaries from slaughterhouse. COCs with more than 3 layers of cumulus cells were selected. COCs were vitrified by two-step procedure using 7.5% and 15% dimethyl sulphoxide (DMSO) as cryoprotective agent (CPA), loaded on Cryotop or French mini-straw, then directly plunged into liquid nitrogen (LN2). Then the COCs containing Cryotop or French mini-straws were warmed in 0.25 M sucrose and 20% FBS-supplemented tissue culture medium (TCM) 199 followed by in vitro culture in 50 μl droplets of bicarbonate-buffered TCM 199 supplemented with 10% FBS, pyruvate, FSH and oestradiol for 24 h at 39°C with 5% CO2 in humidified air. After maturation culture, oocytes were denuded and examined under inverted microscope for presence of polar body as the indication of maturation. The in vitro maturation rate of goat’s oocytes after vitrification and warming was 39.3 ± 6.8%, 31.3 ± 9.4%, 61.6 ± 14.2% when using Cryotop (cryodevice), French mini-straws and without vitrification (control), respectively. Maturation rate was significantly higher (P<0.05) without vitrification. It is suggested that both Cryotop and French mini-straw are efficient cryodevices for vitrification of goat’s oocytes and further investigation is required to optimize the protocol for vitrification and warming procedure for the satisfactory survival of goat’s oocytes. The Bangladesh Veterinarian (2018) 35(1&2): 7-12

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“…These events are defined as oocyte maturation and lead to an ovulated fertilizable oocyte. For IVM, oocytes are incubated for upto 24 h (bovines) [ 23 , 24 ]; 27 h (caprines) [ 22 , 25 ] and 44 h (porcines) [ 26 ] in medium supplemented with hormones (gonadotropins) and growth factors under mineral oil at 39 °C in 5 % CO 2 in humidified air. Gonadotrophins play a major role in triggering resumption of meiosis in the oocyte and expansion of cumulus oophorus.…”

Section: Step By Step Guide To Hmcmentioning

confidence: 99%

Handmade cloning: recent advances, potential and pitfalls

Verma

Arora

Sethi

et al. 2015

J Animal Sci Biotechnol

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Handmade cloning (HMC) is the most awaited, simple and micromanipulator-free version of somatic cell nuclear transfer (SCNT). The requirement of expensive micromanipulators and skilled expertise is eliminated in this technique, proving it as a major revolution in the field of embryology. During the past years, many modifications have been incorporated in this technique to boost its efficiency. This alternative approach to micromanipulator based traditional cloning (TC) works wonder in generating comparable or even higher birth rates in addition to declining costs drastically and enabling cryopreservation. This technique is not only applicable to intraspecies nuclear transfer but also to interspecies nuclear transfer (iSCNT) thus permitting conservation of endangered species. It also offers unique possibilities for automation of SCNT which aims at production of transgenic animals that can cure certain human diseases by producing therapeutics hence, providing a healthier future for the wellbeing of humans. The present review aims at highlighting certain aspects of HMC including recent advancements in procedure and factors involved in elevating its efficiency besides covering the potentials and pitfalls of this technique.

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In vitromaturation and fertilization of prepubertal and pubertal black Bengal goat oocytes

Cited by 16 publications

References 55 publications

Development of prepubertal goat oocytes after theirin vitromaturation and chemical activation

Development of prepubertal goat oocytes after theirin vitromaturation and chemical activation

Vitrification of immature oocytes of goats in Bangladesh

Handmade cloning: recent advances, potential and pitfalls

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