<i>In vitro</i> maturation and ovulation of oocytes of the catfish, <i>Heteropneustes fossilis</i> (Bloch): Effects of mammalian hypophyseal hormones, catfish pituitary homogenate, steroid precursors and metabolites, and gonadal and adrenocortical steroids

Goswami, Shashi V.; Sundararaj, Bangalore I.

doi:10.1002/jez.1401780405

Cited by 83 publications

(12 citation statements)

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“…In these species, cortisol or corticosterone has been reported to promote final maturation of the oocyte [19,37]. In selected species, such maturational effects appear to reflect the direct actions of the corticosteroid per se [37], whereas in others, they seem to result from a synergy with the 20␤-hydroxymetabolites of progesterone [19]. The apparent discrepancy between the above data and the present results may be explained by the differences in experimental animal and/or conditions used.…”

Section: Discussioncontrasting

confidence: 81%

“…To date, the direct effects of glucocorticoids on oocyte maturation have been investigated in several species of fish. In these species, cortisol or corticosterone has been reported to promote final maturation of the oocyte [19,37]. In selected species, such maturational effects appear to reflect the direct actions of the corticosteroid per se [37], whereas in others, they seem to result from a synergy with the 20␤-hydroxymetabolites of progesterone [19].…”

Section: Discussionmentioning

confidence: 95%

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Effects of Glucocorticoids on Maturation of Pig Oocytes and Their Subsequent Fertilizing Capacity In Vitro1

Yang

Chen

1999

Biology of Reproduction

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The aim of this study was to assess the possible role of glucocorticoids in the maturation of pig oocytes and their subsequent fertilizing capacity in vitro. Pig cumulus-enclosed oocytes collected from prepubertal gilts were cultured in Waymouth MB752/1 medium supplemented with sodium pyruvate (50 microg/ml), LH (0.5 microg/ml), FSH (0.5 microg/ml), and estradiol-17beta (1 microg/ml) in the presence or absence of cortisol or dexamethasone (DEX) for 24 h; they then were cultured without hormonal supplements in the presence or absence of cortisol or DEX for an additional 16-24 h. Treatment of cumulus-enclosed or denuded oocytes with increasing concentrations of cortisol or DEX for 48 h resulted in a dose-response inhibition of germinal vesicle breakdown (GVB). Increasing duration (12-48 h) of treatment with DEX (10 microg/ml) led to a time-dependent inhibition of GVB, which achieved statistical significance by 12 h. The addition of DEX (10 microg/ml) to maturation medium immediately after culture or at 12 h, 24 h, or 36 h after culture also decreased the percentage of oocytes with GVB. When oocytes were exposed to DEX for 48 h, the maturation rate was reduced. The degree of this reduction was dependent on DEX, and a concentration of DEX higher than 0.1 microg/ml was needed. The inhibitory effect of DEX on the maturation of oocytes was prevented by the glucocorticoid receptor antagonist RU-486. Exposure of oocytes to DEX for 40 h did not prevent sperm penetration, affect the incidence of polyspermy, or decrease the ability of oocytes to form a male pronucleus. The intracellular concentration of glutathione (GSH) in cumulus-enclosed oocytes was 4.4 mM per oocyte. Exposure of oocytes to DEX (0.01-10 microg/ml) had no effect on GSH concentration. These results demonstrate that glucocorticoids directly inhibit the meiotic but not cytoplasmic maturation of pig oocytes in vitro. This inhibitory effect is not mediated through a decrease in the level of intracellular GSH.

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Section: Discussioncontrasting

confidence: 81%

Section: Discussionmentioning

confidence: 95%

Effects of Glucocorticoids on Maturation of Pig Oocytes and Their Subsequent Fertilizing Capacity In Vitro1

Yang

Chen

1999

Biology of Reproduction

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“…(Katz and Eckstein, 1974). The increase of DOC level was explained by its possible role in the maturation of oocytes and ovulation, in line with the findings of Goswami and Sundararaj (1971) in Heteropneustes fossilis. However, the increased level of the three androgens remained unexplained.…”

supporting

confidence: 87%

Estrogens and estrogenic effects in Tilapia aurea (Cichlidae, Teleostei)

Terkatin-Shimony¹,

Yaron²

1978

Ann. Biol. anim. Bioch. Biophys.

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Summary. Estradiol (E 2 ) level in plasma of non-breeding female Tilapia aurea, measured by RIA, was 0.33: : :!:: 0.02 ng/mi (mean ± SEM, n = 6). This level is about 10 p. 100 of the level found in breeding females, about 4 times higher than in ovariectomized females and similar to the level found in non-breeding males.Twenty-four hours following a single i. p. injection of 0.5 mg E 2 , the estradiol level in plasma of ovariectomized Tilapia was within the physiological range (0,2-10 ng/mi). This level was maintained for at least 4 days. Comparison of the vitellogenic potency of 3 estrogens in ovariectomized T. aurea shows that estriol was more potent than estradiol, which, in turn, was more potent than estrone. Since E 2 increased in the plasma of estrone-injected fish, it is assumed that estrone was converted to estradiol in the recipient fish.Introduction.

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“…maturation and ovulation. In the catfish, cortisol, but not corticosterone, was shown to have MIS activity in vitro (Goswami & Sundararaj 1971). However, in Fundulus heteroclitus, corticosterone induced a high percentage (98%) of GVBD (Greeley et al 1986).…”

Section: Figurementioning

confidence: 99%

Effects of gonadotrophin in vivo and 2-hydroxyoestradiol-17β in vitro on follicular steroid hormone profile associated with oocyte maturation in the catfish Heteropneustes fossilis

Mishra¹,

Joy²

2006

Journal of Endocrinology

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An HPLC method was used to tentatively identify progesterone (P 4 ) and its metabolites (17-hydroxyprogesterone (17-P 4 ) and 17,20 -dihydroxy-4-pregnen-3-one (17,20 -P)), corticosteroids (cortisol and corticosterone) and testosterone in ovary/follicular preparations of the catfish Heteropneustes fossilis associated with in vivo or in vitro oocyte maturation/ovulation. A single i.p. injection of human chorionic gonadotrophin (100 IU/fish, sampled at 0, 8 and 16 h) induced oocyte maturation and ovulation, which coincided with significant and progressive increases in 17,20 -P, and P 4 and 17-P 4 , the precursors of the former. Both cortisol and corticosterone also increased significantly. Conversely, testosterone decreased significantly and progressively over time. Under in vitro conditions, incubation of post-vitellogenic (intact) follicles or follicular envelope (layer) with 2-hydroxyoestradiol (2-OHE 2 , 5 µM for 0, 6 and 24 h) elicited a sharp significant increase in 17,20 -P, the increase being higher in the follicular envelope incubate. P 4 and 17-P 4 also registered significant increases over the time with the peak values at 24 h. Cortisol and corticosterone increased significantly in the intact follicle, but not in the follicular envelope incubate. Testosterone decreased significantly in the intact follicle, but increased significantly (24 h) in the follicular envelope incubate. Coincident with these changes, the percentage of germinal vesicle breakdown (GVBD) increased over the time in the intact follicle incubate (48·9% at 6 h and 79·8% at 24 h). Denuded oocytes on incubation with 2-OHE 2 (5 µM) did not produce any significant change in the percentage of GVBD or in the steroid profile. While corticosterone and 17,20 -P were undetected, P 4 , 17-P 4 , cortisol and testosterone were detected in low amounts. The results show that the 2-OHE 2 -induced GVBD response seems to be mediated through the production of 17,20 -P and corticosteroids. It is suggested that hydroxyoestrogens seem to be a component in the gonadotrophin cascade of regulation of oocyte maturation/ovulation in the catfish.

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In vitro maturation and ovulation of oocytes of the catfish, Heteropneustes fossilis (Bloch): Effects of mammalian hypophyseal hormones, catfish pituitary homogenate, steroid precursors and metabolites, and gonadal and adrenocortical steroids

Cited by 83 publications

References 28 publications

Effects of Glucocorticoids on Maturation of Pig Oocytes and Their Subsequent Fertilizing Capacity In Vitro1

Effects of Glucocorticoids on Maturation of Pig Oocytes and Their Subsequent Fertilizing Capacity In Vitro1

Estrogens and estrogenic effects in Tilapia aurea (Cichlidae, Teleostei)

Effects of gonadotrophin in vivo and 2-hydroxyoestradiol-17β in vitro on follicular steroid hormone profile associated with oocyte maturation in the catfish Heteropneustes fossilis

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