<i>In vitro</i> metabolism of testosterone‐4‐<sup>14</sup>C by canine salivary glands

Mosadoml, Adeyemi; Ofner, P.

doi:10.1111/j.1600-0714.1976.tb01755.x

Cited by 4 publications

(2 citation statements)

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“…Furthermore, in the present work [7a-3H]androstenedione was converted to testosterone, thus demonstrating the presence of 17/?-hydroxysteroid dehydrogenase which has previously been found in the salivary glands of the pig (Kadis & Chyn, 1975) and of other species, e.g. rat and mouse (Baldi & Charreau, 1972), rabbit and man (Cardinali, Denari & Rosner, 1971), man (ElAttar, 1974) and dog (Mosadomi & Ofner, 1976). However, the finding that 3H-labelled DHA and androstenedione were converted to relatively large yields of 5a-androstane-3,17-dione and androsterone indicates that the submaxillary gland of the boar also has the capacity to limit the production of potent androgens.…”

Section: Discussionsupporting

confidence: 85%

“…It is suggested that when testosterone is metabolized primarily to androstenedione as in the submaxillary gland of the dog (Mosadomi & Ofner, 1976) and the rat (Baldi & Charreau, 1972), that the gland in these species is not a significant androgen target organ, and morphological observations tend to support this hypothesis. How¬ ever, when testosterone is primarily reduced to 5<x-products with retention of the 17/?-hydroxy group, this is usually associated with tissues which are known to be a target for androgens (Baulieu, Lasnitzki & Robel, 1968;Ofner, Leav & Cavazos, 1974).…”

Section: Discussionmentioning

confidence: 99%

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Metabolism of Androgens in Vitro by the Submaxillary Salivary Gland of the Mature Domestic Boar

Booth¹

1977

Journal of Endocrinology

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Homogenates of the submaxillary glands of mature domestic boars were incubated with radioactively labelled C19 steroids. The metabolism of [7\g=a\-3H]dehydroepiandrosterone and [7\g=a\-3H]androstenedione was primarily to the end products 5\g=a\-androstane-3,17-dione and androsterone, but some testosterone, 5\g=a\-dihydrotestosterone and 5\g=a\-androstane-3\g=a\,17\g=b\-diol were also formed. When [7\g=a\-3H]5-androstene-3\g=b\,17\g=b\-diol was used as a substrate, the major metabolite was testosterone. Both [7\g=a\-3H]5-androstene-3\g=b\,17\g=b\-diol and [ 1\ g=a\ , 2\ g=a\ -3H] \ x=req-\ testosterone were converted to 5\g=a\-dihydrotestosterone and high yields of 5\g=a\-androstane\x=req-\ 3\g=a\,17\g=b\-diol, but only small yields of 5\g=a\-androstane-3\g=b\,17\g=b\-diol and androsterone were obtained. High yields of 5\g=a\-androstane-3\g=a\,17\g=b\-diol and to a lesser extent 5\g=a\-androstane\x=req-\ 3\g=b\,17\g=b\-diol were formed from the substrate [1\g=a\,2\g=a\-3H]5\g=a\-dihydrotestosterone.Conversely, when both 3H-labelled androstanediols were used as substrates, 5\g=a\-dihydrotestosterone was formed in equal amounts together with a large yield of 5\g=a\-androstane-3\g=a\,17\g=b\-diol from the 3\g=b\-epimer. There was no evidence for steroid sulphatase or aromatase activity in these incubations. These findings support the prohormone concept that precursor steroids of testosterone, known to be present in boar testis, could act as an additional source of potent androgens in peripheral target organs to androgens in this species.In a number of incubations with [1\g=a\,2\g=a\-3H]testosterone, free oestradiol-17\g=b\ and oestrone, but not oestrone sulphate enhanced the formation of androstenedione, but reduced the formation of 5\g=a\-dihydrotestosterone.However, both free and conjugated oestrogen reduced the formation of 5\g=a\-androstane-3\g=a\,17\g=b\-diol. These findings are discussed in relation to the high levels of oestrogen found in male pigs.

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Section: Discussionsupporting

confidence: 85%