<i>In Vitro</i>Morphological Assessment of Apoptosis Induced by Antiproliferative Constituents from the Rhizomes of<i>Curcuma zedoaria</i>

Rahman, Syarifah Nur Syed Abdul; Wahab, Norhanom Abdul; Malek, Sri Nurestri Abd

doi:10.1155/2013/257108

Cited by 121 publications

(75 citation statements)

References 23 publications

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“…Cell apoptosis is a complicated process that is regulated by various factors and signaling pathways, with the two main apoptotic pathways being the intrinsic, or mitochondrial, pathway and the extrinsic pathway [30]. The activation of caspases is a key factor in the signaling pathway that induces cell apoptosis, and the most important caspases are caspase-3, which is often referred to as the pre-caspase for the caspase cascade, and caspase-9 which is regarded as the execution caspase in cell apoptosis [31].…”

Section: Discussionmentioning

confidence: 99%

In Vitro Effects of HAS-2 Gene Silencing on the Proliferation and Apoptosis of the MCF-7 Human Breast Cancer Cell Line

Zhang¹,

Feng²,

Wang³

et al. 2016

Cell Physiol Biochem

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Background: Breast cancer is characterized by a distinct metastatic pattern involving the regional lymph nodes, bone marrow, lung and liver. This study is aimed to investigate the effects of silencing the HAS-2 gene on the proliferation and apoptosis of human breast cancer cells. Methods: MCF-7 cells were collected and assigned into control, scrambled siRNA and HAS-2- siRNA groups. After transfection, the morphological changes in the MCF-7 cells were observed using phase contrast microscopy. qRT-PCR and Western blot assays were used to detect the mRNA and protein expression of apoptosis-related proteins. CCK-8 and flow cytometry were performed to evaluate cell proliferation, the cell cycle and apoptosis. Results: In the control and the scrambled siRNA groups, cells grew adhered to the wall and mainly showed a spindle shape with a clear nucleolus. Compared with the control and scrambled siRNA groups, increases in the number of cells in early apoptosis and metaphase cells in apoptosis were observed in the HAS-2-siRNA group. The HAS-2-siRNA group showed decreased expression of HAS-2 relative to that in the control and scrambled siRNA groups. No significant differences in cell proliferation, cell cycle distribution or apoptosis were noted between the control and scrambled siRNA groups. In the HAS-2-siRNA group, the cell proliferation ability decreased significantly, but the number of cells in the G0/G1 stage, the number of apoptotic cells and the expression of caspase-3 and caspase-9 increased significantly. Conclusion: Our findings indicate that HAS-2 gene silencing may inhibit proliferation and promote apoptosis in the MCF-7 human breast cancer cell line.

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Section: Discussionmentioning

confidence: 99%

In Vitro Effects of HAS-2 Gene Silencing on the Proliferation and Apoptosis of the MCF-7 Human Breast Cancer Cell Line

Zhang¹,

Feng²,

Wang³

et al. 2016

Cell Physiol Biochem

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“…The experiment was realized according to the method described by Syed et al (2013). Briefly, cells were grown in tissue culture dishes and treated with or without the aqueous extract of P. daemia at concentrations (5, 10, 19, 40, 77, 153, 306, 615, 1225, 2450 μg/ml).…”

Section: Methodsmentioning

confidence: 99%

“…After 24 h of incubation in an incubator (37°C in 5% CO2), the cells were harvested and washed with cold PBS (0.1 M, pH 7.4). The cells were suspended in Hoechst 33342 solution (10 μg/ml, Sigma–Aldrich) and were incubated (37°C in 5% CO 2 ) for 7 min (Syed et al, 2013). After incubation with Hoechst 33342, the cells were stained with propidium iodide (2.5 μg/ml, Sigma–Aldrich).…”

Section: Methodsmentioning

confidence: 99%

Antiepileptogenic and Neuroprotective Effects of Pergularia daemia on Pilocarpine Model of Epilepsy

et al. 2017

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In this study, we investigated antiepileptogenic and neuroprotective effects of the aqueous extract of Pergularia daemia roots (PDR) using in vivo and in vitro experimental models. In in vivo studies, status epilepticus caused by pilocarpine injection triggers epileptogenesis which evolves during about 1–2 weeks. After 2 h of status epilepticus, mice were treated during the epileptogenesis period for 7 days with sodium valproate and vitamin C (standards which demonstrated to alter epileptogenesis), or Pergularia daemia. The animals were then, 1 week after status epilepticus, challenged with acute pentylenetetrazole (PTZ) administration to test behaviorally the susceptibility to a convulsant agent of animals treated or not with the plan extract. Memory was assessed after PTZ administration in the elevated plus maze and T-maze paradigms at 24 and 48 h. Antioxidant and acetylcholinesterase activities were determined in the hippocampus after sacrifice, in vitro studies were conducted using embryonic rat primary cortical cultures exposed to L-glutamate. Cell survival rate was measured and apoptotic and necrotic cell death determined. The results showed that chronic oral administration of PDR significantly and dose-dependently increased the latency to myoclonic jerks, clonic seizures and generalized tonic–clonic seizures, and the seizure score. In addition, PDR at all doses (from 4.9 to 49 mg/kg) significantly decreased the initial and retention transfer latencies in the elevated plus maze. Interestingly PDR at the same doses significantly increased the time spent and the number of entries in T-maze novel arm. PDR significantly increased the activities of acetylcholinesterase and antioxidant enzymes superoxide dismutase, catalase, and total glutathione and proteins, and decreased malondialdehyde level. Furthermore, PDR increased viability rate of primary cortical neurons after L-glutamate-induced excitotoxicity, in a dose dependent manner. Altogether these results suggest that PDR has antiepileptogenic and neuroprotective effects, which could be mediated by antioxidant and antiapoptotic activities.

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“…All the cells were cultured using protocols described earlier [14]. MCF7, Ca Ski, A549, and HT29 cells were cultured in supplemented RPMI 1640 medium, HCT116 cells in supplemented McCoy's 5A medium, and MRC5 in supplemented Eagle's minimum essential medium (EMEM).…”

Section: Methodsmentioning

confidence: 99%

Essential Oil Content of the Rhizome ofCurcuma purpurascensBl. (Temu Tis) and Its Antiproliferative Effect on Selected Human Carcinoma Cell Lines

Hong

Lee

Rahman

et al. 2014

The Scientific World Journal

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Curcuma purpurascens Bl., belonging to the Zingiberaceae family, is known as temu tis in Yogyakarta, Indonesia. In this study, the hydrodistilled dried ground rhizome oil was investigated for its chemical content and antiproliferative activity against selected human carcinoma cell lines (MCF7, Ca Ski, A549, HT29, and HCT116) and a normal human lung fibroblast cell line (MRC5). Results from GC-MS and GC-FID analysis of the rhizome oil of temu tis showed turmerone as the major component, followed by germacrone, ar-turmerone, germacrene-B, and curlone. The rhizome oil of temu tis exhibited strong cytotoxicity against HT29 cells (IC50 value of 4.9 ± 0.4 μg/mL), weak cytotoxicity against A549, Ca Ski, and HCT116 cells (with IC50 values of 46.3 ± 0.7, 32.5 ± 1.1, and 35.0 ± 0.3 μg/mL, resp.), and no inhibitory effect against MCF7 cells. It exhibited mild cytotoxicity against a noncancerous human lung fibroblast cell line (MRC5), with an IC50 value of 25.2 ± 2.7 μg/mL. This is the first report on the chemical composition of this rhizome's oil and its selective antiproliferative effect on HT29. The obtained data provided a basis for further investigation of the mode of cell death.

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In VitroMorphological Assessment of Apoptosis Induced by Antiproliferative Constituents from the Rhizomes ofCurcuma zedoaria

Cited by 121 publications

References 23 publications

In Vitro Effects of HAS-2 Gene Silencing on the Proliferation and Apoptosis of the MCF-7 Human Breast Cancer Cell Line

In Vitro Effects of HAS-2 Gene Silencing on the Proliferation and Apoptosis of the MCF-7 Human Breast Cancer Cell Line

Antiepileptogenic and Neuroprotective Effects of Pergularia daemia on Pilocarpine Model of Epilepsy

Essential Oil Content of the Rhizome ofCurcuma purpurascensBl. (Temu Tis) and Its Antiproliferative Effect on Selected Human Carcinoma Cell Lines

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