“…As with previous studies (22,(30)(31)(32)(33)(34)(35)(36), an MHC class II peptide binding predictive algorithm (29) was used to select candidate peptides from the LMP1 sequence that would bind to the products of three common human MHC class II alleles (HLA-DR1, HLA-DR4, and HLA-DR7). Three peptides, LMP1 [35][36][37][38][39][40][41][42][43][44][45][46][47][48][49] (ALLFWLYIVMSDWTG), LMP1 102-116 (GQALFLGIVLFIFGC), and LMP1 159-175 (YLQQNWWT-LLVDLLWLL) were identified as potentially binding the three MHC class II alleles (data not presented), suggesting these could function as promiscuous HTL epitopes. In past studies, we have observed that peptide sequences predicted to bind to HLA-DR1, HLA-DR4, and HLA-DR7 have elicited HTL responses restricted by additional MHC class II alleles, such as DR9, DR13, DR15, or DR53 (22,30,32,(34)(35)(36).…”