<i>In Vitro</i>Phenotypic Variation in Larch Cotyledon Number

Aderkas, Patrick von

doi:10.1086/338824

Cited by 17 publications

(14 citation statements)

References 21 publications

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“…1993), whereas the reduced number of cotyledons caused by long exposure or by high concentrations of PCIB was not due to fused cotyledons. A reduction in the number of cotyledons, similar to the observed, has been reported in somatic embryos of conifers by inclusion of benzyladenine (BA) into the maturation medium (Von Aderkas 2002).…”

Section: Discussionsupporting

confidence: 72%

Effect of anti‐auxins on maturation of embryogenic tissue cultures of Nordmanns fir (Abies nordmanniana)

Grace

Krogstrup

2002

Physiologia Plantarum

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The present study was conducted to improve the transition from proliferation to maturation in embryogenic cultures of Nordmanns fir. For that reason, chemicals reported to affect endogenous levels or activity of auxin were included in the growth media during maturation. The auxin antagonist PCIB reduced proliferation and promoted the development of numerous high-quality mature embryos in the tested cell lines. PCIB could not substitute for exogenously supplied ABA and the positive effect was only found when PCIB and ABA were used in combination. The effect of PCIB was dependent on the concentration and the application period. The auxin transport inhibitor TIBA also reduced proliferation, but had no positive effect on maturation. The auxin synergist phloroglucinol had the opposite effect of PCIB; proliferation was increased and no maturation was initiated. A lowered concentration of boron had no effect on proliferation but had some positive effect on maturation. The optimum protocol for PCIB application was strongly genotype dependent, and a general scheme that covered the tested cell lines could not be found. Overexposure to PCIB during maturation caused abnormal development of the mature embryos, which was revealed by a reduced number of cotyledons. These results suggest that endogenously produced auxin may be one reason for low or failing maturation of embryogenic cultures of Nordmanns fir, but also imply that auxin may play a critical role for proper development of cotyledons during the later stages of embryo maturation.

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Section: Discussionsupporting

confidence: 72%

Effect of anti‐auxins on maturation of embryogenic tissue cultures of Nordmanns fir (Abies nordmanniana)

Grace

Krogstrup

2002

Physiologia Plantarum

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“…Angiosperm monocots and dicots are tightly constrained to form one or two cotyledons, respectively. By contrast, Pinaceae conifers can have highly variable numbers of cotyledons (n c ), both between and within species (von Aderkas, 2002). Average n c for species are reported as low as 3 (in Tsuga) and as high as 9 (in Cedrus) (Butts and Buchholz, 1940).…”

Section: Introductionmentioning

confidence: 99%

Two-stage patterning dynamics in conifer cotyledon whorl morphogenesis

2018

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“…Thus, our results provide support for both a lack of genetic variation and selection as reasons for the current lack of variation in wild radish cotyledon number. Mabberley, 1989;von Aderkas, 2002). Still, the vast majority of plant species produce one or two cotyledons.…”

Section: Introductionmentioning

confidence: 99%

Mechanisms of constraints: the contributions of selection and genetic variance to the maintenance of cotyledon number in wild radish

Conner

Agrawal

2005

J of Evolutionary Biology

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The evolutionary mechanisms underlying the maintenance of invariant traits are poorly understood, partly because the lack of variance makes these mechanisms difficult to study. Although the number of cotyledons that plant species produce is highly canalized, populations of plants frequently contain individuals with abnormal cotyledon numbers. In a garden study with 1857 wild radish plants from 75 paternal half‐sibling families, 89 (almost 5%) had cotyledon numbers less or greater than two. We found evidence for direct selection on cotyledon number, but no evidence for additive genetic variation for cotyledon number. In spite of the very large sample size, our power to detect variation and selection was hampered by the small number of individuals (10) producing more than two cotyledons. Thus, our results provide support for both a lack of genetic variation and selection as reasons for the current lack of variation in wild radish cotyledon number.

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In VitroPhenotypic Variation in Larch Cotyledon Number

Cited by 17 publications

References 21 publications

Effect of anti‐auxins on maturation of embryogenic tissue cultures of Nordmanns fir (Abies nordmanniana)

Effect of anti‐auxins on maturation of embryogenic tissue cultures of Nordmanns fir (Abies nordmanniana)

Two-stage patterning dynamics in conifer cotyledon whorl morphogenesis

Mechanisms of constraints: the contributions of selection and genetic variance to the maintenance of cotyledon number in wild radish

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