In VitroPlant Regeneration from Commercial Cultivars of Soybean

Abstract

Soybean, a major legume crop, is the source of vegetable oil and protein. There is a need for transgenic approaches to breeding superior soybean varieties to meet future climate challenges. Efficient plant regeneration is a prerequisite for successful application of genetic transformation technology. Soybean cultivars are classified into different maturity groups based on photoperiod requirements. In this study, nine soybean varieties belonging to different maturity group were regenerated successfully from three different explants: half split hypocotyl, complete hypocotyl, and cotyledonary node. All the genotypes and explant types responded by producing adventitious shoots. Shoot induction potential ranged within 60–87%, 50–100%, and 75–100%, and regeneration rate ranged within 4.2–10, 2.7–4.2, and 2.6–10.5 shoots per explant using half split hypocotyl, complete hypocotyl, and cotyledonary explants, respectively, among all the tested genotypes. Bunya variety showed the best regeneration response using half split and complete hypocotyl explants and the PNR791 with cotyledonary node. The regenerated shoots were successfully rooted and acclimatized to glasshouse conditions. This study shows that commercial varieties of soybean are amenable to shoot regeneration with high regeneration frequencies and could be exploited for genetic transformation. Further, our results show no correlation between shoots regeneration capacity with the maturity grouping of the soybean cultivars tested.

In Vitro Regeneration Potential of White Lupin (Lupinus albus) from Cotyledonary Nodes

“…We found that, 1 2 M519 media fortified with KT 4.0 mg L −1 + NAA 0.1 mg L −1 showed maximum of 80% shoot regeneration frequency by using HCN explants, while all other combinations also induced shoot formation, albeit with a reduced percentage. Additionally, explant types (HCN, TCN, and WCN) exhibited different responses to different combinations of plant hormones (KT, NAA, and 6-BA), suggesting that, regeneration ability of plant depends on explant type and age [22,61].…”

“…Relatively very poor progress has been accomplished on plant regeneration system in legumes [21], due to the unsuccessful in vitro generation tissue culture systems. Several attempts have previously been made to develop successful regeneration system in legumes such as Glycine max [22], Vigna unguiculata [23], Medicago sativa [24], Phaseolus vulgaris [25] Arachis hypogaea [26] Trifolium resupinatum [27], Lathyrus sativus [28], Vigna aconitifolia [29], Onobrychis viciifolia [30], and Stylosanthes humilis [31], Lupinus angustifolius [32], L. luteus [32][33][34], and L. albus [32,35]. Uhde-Stone et al, demonstrated successful transgenic proteoid root development of white lupin by using Agrobacterium rhizogenes [36].…”

Development of an efficient regeneration system for the precious and fast-growing timber tree <i>Toona ciliata</i>

“…All these influences negatively affect the species growth and practical demand in a large scale. Plant genetic engineering can provide an effective way for genetic improvement (Raza et al 2017). The establishment of genetic transformation system that is a key technique for genetic engineering can be used for plant conservation (Anjusha and Gangaprasad 2016;Chauhan 2016).…”

The Influence of Seed Viability on the Germination and In Vitro Multiple Shoot Regeneration of Soybean (Glycine max L.)

“…In plant tissue culture, the effect of seed viability is not fully researched, especially its role in influencing seed germination, normal seedling development and vigour of seedlings from which cotyledonary explants are derived. Many authors, including Keneda et al [6], Paz et al [7], Shelar et al [8], Hartmann et al [9]and Raza et al [10] have highlighted the difficulties faced during in vitro regeneration of soybean, especially with regard to genotype specificity. These reports, as well as many others, paid less attention to the loss of seed viability, which occurs not only in soybean but even in Galenia pubescens, Swertia chirayita and Zea mays [11][12][13].Therefore, the objective of this research was to determine the effects that seed viability has on germination, seedling vigour and multiple shoot induction.…”

“…and Raza et al [10] have highlighted the difficulties faced during in vitro regeneration of soybean, especially with regard to genotype specificity. These reports, as well as many others, paid less attention to the loss of seed viability, which occurs not only in soybean but even in Galenia pubescens, Swertia chirayita and Zea mays [11][12][13].…”