<i>In vitro</i> production of polyhydroxyalkanoates: achievements and applications

Thomson, Nicholas M.; Roy, Ipsita; Summers, David; Sivaniah, Easan

doi:10.1002/jctb.2299

Cited by 41 publications

(35 citation statements)

References 81 publications

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“…In fact, CoA is known to inhibit both pha A and pha C , while pha B is inhibited by NADP + . Therefore polymer biosynthesis is highly reduced if any of these are present in large amounts (Satoh et al , 2003; Thomson et al , 2010). …”

Section: Resultsmentioning

confidence: 99%

Growth kinetics, effect of carbon substrate in biosynthesis of mcl-PHA by Pseudomonas putida Bet001

Gumel¹,

Annuar²,

Heidelberg³

2014

Braz. J. Microbiol.

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Growth associated biosynthesis of medium chain length poly-3-hydroxyalkanoates (mcl-PHA) in Pseudomonas putida Bet001 isolated from palm oil mill effluent was studied. Models with substrate inhibition terms described well the kinetics of its growth. Selected fatty acids (C8:0 to C18:1) and ammonium were used as carbon and nitrogen sources during growth and PHA biosynthesis, resulting in PHA accumulation of about 50 to 69% (w/w) and PHA yields ranging from 10.12 g L−1 to 15.45 g L−1, respectively. The monomer composition of the PHA ranges from C4 to C14, and was strongly influenced by the type of carbon substrate fed. Interestingly, an odd carbon chain length (C7) monomer was also detected when C18:1 was fed. Polymer showed melting temperature (Tm) of 42.0 (± 0.2) °C, glass transition temperature (Tg) of −1.0 (± 0.2) °C and endothermic melting enthalpy of fusion (ΔHf) of 110.3 (± 0.1) J g−1. The molecular weight (Mw) range of the polymer was relatively narrow between 55 to 77 kDa.

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Section: Resultsmentioning

confidence: 99%

Growth kinetics, effect of carbon substrate in biosynthesis of mcl-PHA by Pseudomonas putida Bet001

Gumel¹,

Annuar²,

Heidelberg³

2014

Braz. J. Microbiol.

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“…Acrylates easily form polymers because the double bonds are very reactive. Blends of synthetic polymers and PHA offer cost performance benefits because acrylate is cheap and available year-round (Thomson et al 2010). Blending of PHA with Acrylate can lower the cost as well as it can also control the mechanical properties of the blend.…”

Section: Introductionmentioning

confidence: 99%

Biodegradability studies of polyhydroxyalkanoate (PHA) film produced by a marine bacteria using Jatropha biodiesel byproduct as a substrate

Shrivastav

Mishra

Pancha

et al. 2010

World J Microbiol Biotechnol

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Polyhydroxyalkanoates are water-insoluble, hydrophobic polymers and can be degraded by microorganisms that produce extracellular PHA depolymerase. The present work was aimed to evaluate the degradability of Polyhydroxyalkanoate film produced by Halomonas hydrothermalis using Jatropha biodiesel byproduct as a substrate. PHB films were subjected to degradation in soil and compared with the synthetic polymer (acrylate) and blend prepared using the synthetic polymer (acrylate) and PHB. After 50 days, 60% of weight loss in PHB film and after 180 days 10% of blended film was degraded while no degradation was found in the synthetic film. Scanning electron microscopy and confocal microscopy revealed that after 50 days the PHB film and the blended film became more porous after degradation while synthetic film was not porous.The degradative process was biologically mediated which was evident by the control in which the PHB films were kept in sterile soil and the films showed inherent integrity over time. The TGA and DSC analysis shows that the melting temperatures were changed after degradation indicating physical changes in the polymer during degradation.

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“…There is interest in developing in vitro PHA production processes (4). This would facilitate the development of continuous production systems, eliminate the costly and environmentally damaging process of PHA recovery from within bacterial cells, allow the polymerization of monomeric units that are not easily produced by bacteria, and extend the range of physical properties of the final product.…”

mentioning

confidence: 99%

Efficient Production of Active Polyhydroxyalkanoate Synthase in Escherichia coli by Coexpression of Molecular Chaperones

Thomson

Saika

Ushimaru

et al. 2013

Appl Environ Microbiol

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cThe type I polyhydroxyalkanoate synthase from Cupriavidus necator was heterologously expressed in Escherichia coli with simultaneous overexpression of chaperone proteins. Compared to expression of synthase alone (14.55 mg liter ؊1 ), coexpression with chaperones resulted in the production of larger total quantities of enzyme, including a larger proportion in the soluble fraction. The largest increase was seen when the GroEL/GroES system was coexpressed, resulting in approximately 6-fold-greater enzyme yields (82.37 mg liter ؊1 ) than in the absence of coexpressed chaperones. The specific activity of the purified enzyme was unaffected by coexpression with chaperones. Therefore, the increase in yield was attributed to an enhanced soluble fraction of synthase. Chaperones were also coexpressed with a polyhydroxyalkanoate production operon, resulting in the production of polymers with generally reduced molecular weights. This suggests a potential use for chaperones to control the physical properties of the polymer.

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In vitro production of polyhydroxyalkanoates: achievements and applications

Cited by 41 publications

References 81 publications

Growth kinetics, effect of carbon substrate in biosynthesis of mcl-PHA by Pseudomonas putida Bet001

Growth kinetics, effect of carbon substrate in biosynthesis of mcl-PHA by Pseudomonas putida Bet001

Biodegradability studies of polyhydroxyalkanoate (PHA) film produced by a marine bacteria using Jatropha biodiesel byproduct as a substrate

Efficient Production of Active Polyhydroxyalkanoate Synthase in Escherichia coli by Coexpression of Molecular Chaperones

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