In Vitro Propagation Of ‘Gisela 5’ Rootstock As Affected By Mineral Composition Of Media And Plant Growth Regulators

Abstract

The purpose of the presented study was to determine the best mineral composition of media and plant growth regulators in the micropropagation of the ‘Gisela 5’ (Prunus cerasus × P. canescens) dwarf sweet cherry rootstock. Nodal explants from young healthy shoots were excised and cultured on Murashige and Skoog medium without growth regulators. In vitro raised shoot tips were transferred to three culture media including Murashige and Skoog (MS), Driver and Kuniyuki (DKW) and Lloyd and McCown (WPM) containing benzyl adenine (BA) (0.5, 1 or 2 mg·l−1) in combination with kinetin (Kin) at 0 or 0.5 mg·l−1. WPM and DKW media were proving to be the most effective, resulting in a higher percentage of shoot multiplication and shoot number as compared to MS. BA in concentration 2 mg·l−1 resulted in the highest number of microshoots per explant (3.1). For rooting, 0, 0.5, 1 or 2 mg·l−1 indole-3-butyric acid (IBA) on MS, DKW and WPM media were tested. WPM medium containing 2 mg·l−1 IBA was most effective for rooting (93.7%) in comparison to MS (53.1%) and DKW (14.0%). Rooted plantlets were successfully hardened and established in pots.

First Report of Krymsk® 5 (cv. VSL 2) Cherry Rootstock In Vitro Propagation: Studying the Effect of Cytokinins, Auxins and Endogenous Sugars

“…As far the root number is concerned, the highest number was obtained by the combinations 5 μΜ IBA -2.5 μΜ NAA, 20 μΜ IBA -2.5 μΜ NAA, 20 μΜ IBA -5 μΜ NAA, 20 μΜ IBA -10 μΜ NAA and 10 μΜ NAA, indicating that IBA and NAA may act synergistically on root elongation. Increasing IBA concentration did not increase root number, unlike the results reported by Fotopoulos and Sotiropoulos, (2005) and Fallahpour et al (2015) in PR 204/84 and GiSelA® 5 respectively. The highest root length was observed under IBA at 5 μΜ and the lowest under the equimolar addition of both auxins at 5 μM.…”

In Vitro Propagation of Some Mahaleb Genotypes as Candidate Rootstock for Sweet Cherries

“…According to the average of years, root length was determined to be between 11.00 mm and 41.69 mm. In in vitro replication studies of the Prunus species, the root length reported as (Sülüşoğlu and Çavuşoğlu, 2013) 1.68-3.29 cm; (Hosseinpour et al, 2015) 1-2.03 cm and (Fallahpour et al, 2015) 1.5-3.1 cm. The results we obtained from the trial are in agreement with the results of the studies on this subject.…”

Adjustment of culture media to optimize in vitro rhizogenesis of domestic plums of various origins

“…Among the most harmful diseases of plum sharka (smallpox) (PPV) -Plum pox potyvirus is and now has become a limiting factor in the cultivation of plum fruits due to their premature abscission or deterioration in quality, leading to yield losses of up to 85-100% [5,6] Due to the fact that the domestic plum is a hexaploid, cultigenic species, it is necessary to take this feature into account when working with it in an in vitro culture, selecting for each stage of subcultivation, an individual set of components of the nutrient medium. Over the past decade, in the literature, there are data concerning the experiments on the culture of isolated tissues of certain varieties of plum [4,[7][8][9][10][11].…”

“…The rooting stage is crucial and precedes the adaptation process. Higher efficiency of rhizogenesis of plum varieties on a nutrient artificial medium -MS -Murashige and Skoog (1962), as indicated by the authors working in this direction, there is a need to adjust it taking into account varietal characteristics [5,[7][8][9]. For example, a two-fold diluted MS nutrient medium is proposed [12,13].…”