<i>In vitro</i> properties of red cells prepared from half‐strength citrate CPD/RAS‐2 (Erythro‐Sol) donations in PL‐146 plastic

Hornsey,; MacDonald, Jeffrey R.; Drummond,; Walker, Trent; Prowse,

doi:10.1046/j.1365-3148.2000.00229.x

Cited by 10 publications

(5 citation statements)

References 18 publications

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“…It is observed that, parameters such as 2,3-DPG, ATP, pH, surrounding medium Na + , K + and Cl − concentrations were measured within the range of published data (figure 6) (Weisbach et al 2000, Hornsey et al 2000. Stored RBCs were depleted of 2,3-DPG, following the 1st or 2nd week of storage (figure 6(a)) and they have a left-shifted oxygen dissociation curve, greater oxygen affinity, and supply less oxygen to tissues .…”

Section: Effects Of Physiological Parameters On the Electrical Proper...supporting

confidence: 67%

“…As shown in figure 6(b), under storage conditions, ATP fell down remarkably as the red blood cells have no mitochondria and they cannot regenerate adenosine triphosphate (Hornsey et al 2000). The mean ATP value on day 0 was 5.4 ± 0.5 µmol gHb −1 and a gradual decrease was observed with 1.6 ± 0.5 µmol gHb −1 (30%) on day 35.…”

Section: Effects Of Physiological Parameters On the Electrical Proper...mentioning

confidence: 94%

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Storage effects on the Cole-Cole parameters of erythrocyte suspensions

2006

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Chemical alterations of red blood cells (RBCs) during storage eventually affect the electrical properties of blood. In this study, the physiological parameters such as extracellular (SAGM + CPD + residual plasma) Na(+), K(+), Cl(-), pH, 2,3-DPG and ATP together with the Cole-Cole parameters were measured using erythrocyte suspensions from 51 male donors (31 donors form the training set and 20 donors are used for testing), on the 0th, 10th, 21st, 35th and 42nd days of storage. During storage, while the surrounding fluid resistance (R(e)) and the effective cell membrane capacitance (C(m)) increased progressively with time, the intracellular fluid resistance (R(i)) has decreased. Storage of RBCs resulted in a rise in K(+) and a fall in Na(+), Cl(-), pH, 2,3-DPG and ATP. Accordingly, electrical parameters were all correlated with Na(+), K(+), Cl(-), pH and ATP at varying levels. By applying multi-regression analysis, it is concluded that R(i), R(e) and C(m) are appropriate for modeling Na(+), K(+), Cl(-), pH and ATP during storage.

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Section: Effects Of Physiological Parameters On the Electrical Proper...supporting

confidence: 67%

Section: Effects Of Physiological Parameters On the Electrical Proper...mentioning

confidence: 94%

Storage effects on the Cole-Cole parameters of erythrocyte suspensions

2006

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“…The mean extracellular pH of 7,5 ± 0,2 (day 0) diminished slightly to 6,5 ± 0,2 by day 42. As shown in Figure 3(g), in storage conditions, ATP fell down remarkably as the red blood cells have no mitochondria and they can not regenerate adenosine triphosphate [11]. The mean ATP value on day 0 was 5,4 ± 0,5 μmol/gHb and a gradual decrease was observed with 1,6 ± 0,5 μmol/gHb (30%) on day 35.…”

Section: Resultsmentioning

confidence: 93%

“…3) [10][11]. Stored red blood cells (RBC) were depleted of 2,3-DPG, following the 1st or 2nd week of storage and they have a left-shifted oxygen dissociation curve, greater oxygen affinity, and supply less oxygen to tissues [12].…”

Section: Resultsmentioning

confidence: 99%

Effects of Physiological Parameters on the Electrical Properties of Blood Bank Stored Erythrocyte Suspensions

Sezdi¹,

Ülgen²

2006

2006 International Conference of the IEEE Engineering in Medicine and Biology Society

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In this study, the physiological parameters such as extracellular (SAGM + CPD + residual plasma) Na + , K + , Cl -, pH, 2,3-DPG and ATP together with the Cole-Cole parameters were measured using erythrocyte suspensions from 51 male donors (31 donors form the training set and 20 donors are used for testing), on the 0 th , 10 th , 21 st , 35 th and 42 nd days of storage. Accordingly, electrical parameters were all correlated with Na + , K + , Cl -, pH and ATP, at varying levels. By applying the multi-regression analysis, it is concluded that R i , R e and C m are appropriate for modeling Na + , K + , Cl -, pH and ATP during blood bank storage and predicting blood quality.

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“…As shown in Table 1, in storage conditions, ATP fell down remarkably as the red blood cells have no mitochondria and they could not regenerate adenosine triphosphate [16]. The mean ATP value on day 0 was 5.1 ± 0.4 lmol/gHb and on the 21st day, 54% of the mean initial value was presented.…”

Section: Resultsmentioning

confidence: 97%

Physiological quality assessment of stored whole blood by means of electrical measurements

Ülgen

Sezdi

2007

Med Bio Eng Comput

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The physiological parameters of blood such as extracellular Na + , K + , Cl -, pH, 2,3-DPG and ATP and the complex electrical impedance were measured using whole blood samples from 31 male donors (21 donors form the training set and ten donors were used for testing), on the 0th, 10th and 21st day of blood bank storage. During storage, while the extracellular fluid resistance (R e ) and the intracellular fluid resistance (R i ) decreased progressively with time, the effective cell membrane capacitance (C m ) has increased. Blood bank storage resulted in a rise in K + and a fall in Na + , Cl -, pH, 2,3-DPG and ATP. Accordingly, all electrical parameters correlated with Na + , K + , Cl -, pH and ATP, at varying levels. By applying the multi-regression analysis, it was demonstrated that R i , R e and especially C m were appropriate for the assessment of Na + , K + , Cl -, pH and ATP until the 21st day of storage.

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In vitro properties of red cells prepared from half‐strength citrate CPD/RAS‐2 (Erythro‐Sol) donations in PL‐146 plastic

Cited by 10 publications

References 18 publications

Storage effects on the Cole-Cole parameters of erythrocyte suspensions

Storage effects on the Cole-Cole parameters of erythrocyte suspensions

Effects of Physiological Parameters on the Electrical Properties of Blood Bank Stored Erythrocyte Suspensions

Physiological quality assessment of stored whole blood by means of electrical measurements

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