In vitro release kinetics of proteins from bioactive foams

Abstract

This study describes an approach to obtaining 3-D scaffolds for tissue engineering that allows the incorporation and release of biologically active proteins to stimulate cell function. Laminin was adsorbed on the textured surfaces of binary 70S30C (70 mol % SiO(2), 30 mol % CaO) and ternary 58S (60 mol % SiO(2), 36 mol % CaO, 4 mol % P(2)O(5)) foams. The covalent bonds between the binding sites of the proteins and the ligands on the scaffolds' surfaces did not denaturate the proteins. In vitro studies show that the foams modified with chemical groups and coated with laminin were bioactive, as demonstrated by the formation of a crystalline hydroxy carbonate apatite (HCA) layer formed on the surfaces of the foams upon exposure to simulated body fluid (SBF). The release of proteins from the foams also was investigated. Sustained and controlled release from the scaffolds over a 30-day period was achieved. Laminin release from the bioactive foams followed the dissolution rate of the material network. These results suggest that bioactive foams have the potential to act as scaffolds for soft-tissue engineering with a controlled release of proteins that can induce tissue formation or regeneration.

Nanoporosity Significantly Enhances the Biological Performance of Engineered Glass Tissue Scaffolds

“…Note that the optimal concentration of ionic products of dissolution, especially the silicate and Ca 2 + ions released from a BG surface, is important for bone regeneration. 3 On the other hand, proteins can bond to the BG surface, 58 and thus mediate cell attachment, migration, and growth. 29 These surface area-regulated protein absorption and ion release processes can greatly affect the performance of a biomaterial.…”

New trends in bioactive scaffolds: The importance of nanostructure

“…Sol-gel glasses can then easily be functionalised with groups such as mercapto-and amino-groups 24 that can be used to covalently bond proteins to the surface of the glasses. 25 Specific nanopore shapes and sizes may also affect which proteins are adsorbed to the glass surface. This can be used in a variety of applications.…”

Gene Therapy Progress and Prospects: In tissue engineering

“…1,2,16 The hierarchical structure of the bioactive foams can be tailored for tissue bonding, resorption and delivery of dissolution products that provide the controlled rates of release of osteogenic stimuli, [68][69][70] and the nanopores can be modified with various proteins to control cell integrin-scaffold interactions. 71,72 The calcia-silica-based materials are easily and economically processed to ISO standards 69 and have been used clinically for 20 years with FDA and CE regulatory clearance. Our studies have shown that the inorganic ionic dissolution products released during controlled resorbtion of the gel-glass scaffolds stimulate expression of genes and enhance osteogenesis in mouse and human ES cells, 16 adult human stem cells 1 and human foetal bone cells.…”