In vitro renin–angiotensin system inhibition and in vivo antihypertensive activity of peptide fractions from lima bean (<scp>Phaseolus lunatus</scp> L.)

Ciau-Solís, Norma; Acevedo-Fernández, Juan José; Betancur‐Ancona, David

doi:10.1002/jsfa.8543

Cited by 51 publications

(37 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The results generally suggest that pigeon pea hydrolysates may have potential as antihypertensive agents. The results for PPHPp were higher than 10.53% reported for pepsin‐pancreatin lima bean hydrolysate (Ciau‐Solís, Acevedo‐Fernández, & Betancur‐Ancona, ). The observed differences in the ACE‐inhibitory activity have been attributed to differences in legume type, peptide mixture composition, changes in the parameters of the digestion process (enzyme, pH, time, temperature, substrate/enzyme ratio, etc.)…”

Section: Resultscontrasting

confidence: 62%

See 1 more Smart Citation

Antioxidant properties, ACE/renin inhibitory activities of pigeon pea hydrolysates and effects on systolic blood pressure of spontaneously hypertensive rats

Olagunju

Omoba

Enujiugha

et al. 2018

Food Science & Nutrition

View full text Add to dashboard Cite

Legumes are rich sources of protein in human diet and their consumption has been associated with the prevention of chronic diseases attributable to their bioactive components. Pigeon pea (Cajanus cajan) is an underutilized legume with relatively high protein content (~24%). Protein hydrolysates were prepared from pea isolate by enzymatic hydrolysis using pepsin and pancreatin. Hydrolysates were evaluated for their amino acid composition, antioxidant properties, in vitro and in vivo antihypertensive properties. The hydrolysates had high hydrophobic amino acids, especially isoleucine, phenylalanine, and leucine. Pepsin‐pancreatin‐hydrolyzed pea protein (PPHPp) showed significantly higher ability to scavenge DPPH˙ while pancreatin‐hydrolyzed pea protein (PPHPa) had higher ˙OH, ABTS˙+ scavenging, Fe3+ reducing and linoleic acid peroxidation inhibition. PPHPp exhibited superior angiotensin‐converting enzyme inhibition (61.82%) while PPHPa showed higher renin inhibition (14.28%). PPHPp exhibited strong antihypertensive effect, showing an instantaneous systolic blood pressure lowering effect (−26.12 mmHg) within 2‐h post‐oral administration. Pigeon pea protein hydrolysate (especially from pancreatin digest) could therefore, be a promising source of bioactive peptides and potential ingredient for formulation of functional foods against oxidative stress and hypertension.

show abstract

Section: Resultscontrasting

confidence: 62%

“…Ciau‐Solís et al. () reported a renin inhibitory activity of 5.5% for lima bean hydrolysate obtained using pepsin‐pancreatin sequential hydrolysis.…”

Section: Resultsmentioning

confidence: 99%

Antioxidant properties, ACE/renin inhibitory activities of pigeon pea hydrolysates and effects on systolic blood pressure of spontaneously hypertensive rats

Olagunju

Omoba

Enujiugha

et al. 2018

Food Science & Nutrition

View full text Add to dashboard Cite

show abstract

“…In this regard, Ciau‐Solís et al . () reported that peptide fractions from P. lunatus hydrolysate with molecular weight >1000 Da had higher ACE‐I inhibitory activity than those with < 1000 Da. However, further purification and physicochemical characterisation studies of ACE‐I inhibitory peptides from PG‐C dialysate must be done to confirm these results.…”

Section: Resultsmentioning

confidence: 99%

Pyropia columbina phycocolloids as microencapsulating material improve bioaccessibility of brewers’ spent grain peptides with ACE‐I inhibitory activity

Cian

Salgado

Mauri

et al. 2019

Int J of Food Sci Tech

View full text Add to dashboard Cite

Summary The effects of wall hydrocolloids on delivery and bioaccessibility of encapsulated brewers’ spent grain ACE‐I inhibitory peptides after simulated gastrointestinal digestion were assessed. Microencapsulation of peptides was carried out by spray drying using locust bean gum, P. columbina phycocolloids, or its mixtures as wall materials. Microcapsules presented round external surfaces with some concavities, negative surface charge and encapsulation efficiencies higher than 90%. The incorporation of phycocolloids to formulations increased the encapsulation efficiency, negative surface charge and resistance against digestive enzymes of microcapsules. Encapsulated peptides with P. columbina phycocolloids showed lower IC50 value of ACE‐I inhibition than un‐encapsulated peptides (2.4 ± 0.2 vs. 7.2 ± 0.3 mg mL−1 protein), but higher than that obtained for hydrolysate (1.5 ± 0.2 mg mL−1 protein), indicating a 75% protection of bioactivity. Electrostatic and hydrophobic interactions between P. columbina phycocolloids and brewers’ spent grain peptides could be implicated in the protection of peptides during gastrointestinal digestion.

show abstract

“…P. lunatus hydrolysate was obtained sequentially by Alcalase ® and Flavourzyme ® hydrolysis according to Ciau‐Solís et al . (). Degree of hydrolysis (DH) was calculated according to Nielsen et al .…”

Section: Methodsmentioning

confidence: 97%

“…A protein concentrate was made according to Betancur-Ancona et al (2004). P. lunatus hydrolysate was obtained sequentially by Alcalase Ò and Flavourzyme Ò hydrolysis according to Ciau-Sol ıs et al (2018). Degree of hydrolysis (DH) was calculated according to Nielsen et al (2001).…”

Section: Protein Hydrolysatementioning

confidence: 99%

Bioactive Phaseolus lunatus peptides release from maltodextrin/gum arabic microcapsules obtained by spray drying after simulated gastrointestinal digestion

Cian

Campos‐Soldini

Chel‐Guerrero

et al. 2018

Int J of Food Sci Tech

Self Cite

View full text Add to dashboard Cite

Bioactive peptides can be protected from the gastrointestinal environment by microencapsulation. The aim was to assess the effect of simulated gastrointestinal digestion on bioactive properties of P. lunatus peptides (ACE‐I, α‐glucosidase, α‐amylase, and dipeptidyl peptidase‐IV inhibition) microencapsulated by spray drying using maltodextrin/gun arabic in optimal formula. Microcapsules were formulated using a 22 factorial design. The simultaneous effect of P. lunatus hydrolysate (4 and 10 g 100 g−1) and proportion of maltodextrin/gum arabic (25:75 and 75:25 g 100 g−1) on yield, protein efficiency, size, protein release at pH 2.0 and pH 7.0 of the different microcapsules was studied. Microcapsules with maximum yield, protein efficiency, and protein release at pH 7.0, and minimum protein release at pH 2.0 were subjected to simulated gastrointestinal digestion. Microcapsules obtained in optimal formula preserved the α‐glucosidase, α‐amylase, and dipeptidyl peptidase‐IV inhibitory activity of P. lunatus peptides after in vitro gastrointestinal digestion.

show abstract

In vitro renin–angiotensin system inhibition and in vivo antihypertensive activity of peptide fractions from lima bean (Phaseolus lunatus L.)

Abstract: Peptide fractions from lima bean Phaseolus lunatus protein hydrolysates exhibit both in vitro and in vivo antihypertensive activity. Bioactive peptides from lima bean have potential applications as ingredients in functional foods. © 2017 Society of Chemical Industry.

Cited by 51 publications

References 24 publications

Antioxidant properties, ACE/renin inhibitory activities of pigeon pea hydrolysates and effects on systolic blood pressure of spontaneously hypertensive rats

Antioxidant properties, ACE/renin inhibitory activities of pigeon pea hydrolysates and effects on systolic blood pressure of spontaneously hypertensive rats

Pyropia columbina phycocolloids as microencapsulating material improve bioaccessibility of brewers’ spent grain peptides with ACE‐I inhibitory activity

Bioactive Phaseolus lunatus peptides release from maltodextrin/gum arabic microcapsules obtained by spray drying after simulated gastrointestinal digestion

Contact Info

Product

Resources

About