<i>In vitro</i> rescue immature oocytes – a literature review

Jie, Huiying; Zhao, Minyi; Alqawasmeh, Odai; Chan, Carol Pui Shan; Lee, Tin‐Lap; Li, Tin‐Chiu; Chan, David Yiu Leung

doi:10.1080/14647273.2021.1876932

Cited by 17 publications

(12 citation statements)

References 83 publications

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“…Rescue IVM of GV oocytes from conventional IVF cycles is an additional consideration [38] (Fig. 4D), although it is not considered a clinical IVM procedure as it is a non-recommended and unconventional clinical practice [39,40]. Table 1 briefly outlines the pros and cons of these different approaches to IVM.…”

Section: Laboratory and Clinical Approaches To Ivmmentioning

confidence: 99%

“…D This is the maturation in vitro of immature GV-stage oocytes collected from conventional IVF cycles after OS and ovulation triggering, mostly with hCG [38]. These are commonly regarded as medically unusable oocytes and are usually discarded in most IVF clinics [39]. These oocytes are usually naked, as oocytes are denuded of cumulus cells after OPU prior to ICSI; hence, rescue IVM oocytes are invariably cultured in a denuded state from the GV to MII stage in vitro environment that allows the acquisition of developmental competence for the oocyte over~24 h in the pre-IVM step, and (d) elicit resumption of and progression of meiosis under conditions mimicking the post-LH surge follicular environment (Fig.…”

Section: Recapitulating the Follicle Environment: The Evolution Of Biphasic Ivmmentioning

confidence: 99%

“…• Relatively high MII rates at~70% [46] • Oocytes are collected at a mixture of meiotic stages [47] • Additional laboratory burden due to at least two rounds of ICSI per OPU [18] • IVM of GVB oocyte cohort is suboptimal as the exact extent of their meiotic progression at start cannot be determined [47] • Modest live birth rates [41,47] • Prohibitive to the use of any pre-IVM culture approach [34] Rescue IVM • GV oocytes are common in conventional IVF patients • May generate additional embryos for transfer [38] • Oocytes commonly have meiotic defects [48] • IVM in the absence of cumulus cell support → poor oocyte quality [20,27,40] • Questionable safety [39,40] majority of IVM treatment cycles after a short FSH-priming protocol for a least 3 days, with satisfactory pregnancy outcomes, either using a non-triggered IVM protocol [65] or hCG-triggered IVM protocol [66]. Nevertheless, based on the observation, again with retrospective data, that live birth rates after frozen ET (FET) were higher compared to fresh ET [53,54], it was suggested that endometrial quality in non-hCG-triggered IVM cycles may be suboptimal for normal embryo implantation.…”

Section: Advantages Disadvantagesmentioning

confidence: 99%

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Perspectives on the development and future of oocyte IVM in clinical practice

Vos

Grynberg

et al. 2021

J Assist Reprod Genet

112

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Oocyte in vitro maturation (IVM) is an assisted reproductive technology designed to obtain mature oocytes following culture of immature cumulus–oocyte complexes collected from antral follicles. Although IVM has been practiced for decades and is no longer considered experimental, the uptake of IVM in clinical practice is currently limited. The purpose of this review is to ensure reproductive medicine professionals understand the appropriate use of IVM drawn from the best available evidence supporting its clinical potential and safety in selected patient groups. This group of scientists and fertility specialists, with expertise in IVM in the ART laboratory and/or clinic, explore here the development of IVM towards acquisition of a non-experimental status and, in addition, critically appraise the current and future role of IVM in human ART.

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Section: Laboratory and Clinical Approaches To Ivmmentioning

confidence: 99%

Section: Recapitulating the Follicle Environment: The Evolution Of Biphasic Ivmmentioning

confidence: 99%

Section: Advantages Disadvantagesmentioning

confidence: 99%

See 1 more Smart Citation

Perspectives on the development and future of oocyte IVM in clinical practice

Vos

Grynberg

et al. 2021

J Assist Reprod Genet

112

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“…By this technique, oocytes are retrieved before the first meiosis is completed, and thus CoQ10 supplementation to culture media at this point may improve their oxidative status, enhance the mitochondrial function, and, subsequently, better their overall quality. Alternatively, immature oocytes deriving from conventional stimulation cycles, which have received ovulation trigger in vivo but have not completed meiosis I when retrieved, may benefit from this treatment [41].…”

Section: In Vitro Maturation Culturementioning

confidence: 99%

Does Coenzyme Q10 Supplementation Improve Human Oocyte Quality?

Rodríguez-Varela

Labarta

2021

IJMS

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Acquiring oocyte competence requires optimal mitochondrial function and adequate ATP levels. In this context, CoQ10 supplementation may improve human oocyte quality and subsequent reproductive performance given its role in ATP synthesis and mitochondrial protection from ROS oxidative damage. In infertility treatments, CoQ10 therapy can be orally supplied to promote a more favorable environment for oocyte development in vivo or by its addition to culture media in an attempt to improve its quality in vitro. Human clinical studies evaluating the impact of CoQ10 on reproductive performance are summarized in this review, although the available data do not clearly prove its ability to improve human oocyte quality. The main objective is to provide readers with a complete overview of this topic’s current status as well as the keys for potential future research lines that may help to take this therapy to clinical practice. Indeed, further clinical trials are needed to confirm these results along with molecular studies to evaluate the impact of CoQ10 supplementation on oxidative stress status and mitochondrial function in human gametes.

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“…A controlled ovarian stimulation (COS) treatment using gonadotropins is required for the development of multiple follicles in one treatment cycle, which is the standard treatment needed to obtain multiple mature oocytes for cycles aimed at in vitro fertilization (IVF). At many centers today, only mature oocytes are vitrified, but after oocyte retrieval around 15% of retrieved oocytes are found immature, and these are discarded without further utilization [ 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

Exploring the Developmental Potential of Human Germinal Vesicle Oocytes Aiming at Fertility Preservation: Can We Increase the Yields of Competent Oocytes through IVM Combined with Vitrification?

Hao

Phoon

Barbunopulos

et al. 2022

JCM

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The rescue in vitro maturation (rIVM) of germinal vesicle oocytes (GVs) has been proposed to improve the total number of mature oocytes in women undergoing fertility preservation. Currently, there is no consensus about the clinical utility of this practice, and heterogeneity in the protocols used may influence the final outcomes. This study investigated the developmental potential of mature metaphase II (MII) human oocytes obtained from GVs after rIVM and the impact of applying vitrification at different timepoints either before or after rIVM. After randomization, oocytes were assigned to undergo rIVM and thereafter vitrification or intracytoplasmic sperm injection (ICSI), or to undergo direct vitrification-warming and thereafter rIVM and ICSI. The likelihood of obtaining MII oocytes was just slightly higher in the fresh rIVM group compared to the vitrification-warming-rIVM group. When comparing fresh rIVM that underwent subsequently ICSI, the fertilization and developmental rates up to the blastocyst stage were seen to be reduced in both groups that underwent vitrification either before or after rIVM. Although some blastocysts were obtained in the fresh rIVM-ICSI group, the efficacy of these methods was low overall, suggesting that the further development of protocols for IVM conducted early after denudation is needed to improve the final results of rIVM aiming at fertility preservation.

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In vitro rescue immature oocytes – a literature review

Cited by 17 publications

References 83 publications

Perspectives on the development and future of oocyte IVM in clinical practice

Perspectives on the development and future of oocyte IVM in clinical practice

Does Coenzyme Q10 Supplementation Improve Human Oocyte Quality?

Exploring the Developmental Potential of Human Germinal Vesicle Oocytes Aiming at Fertility Preservation: Can We Increase the Yields of Competent Oocytes through IVM Combined with Vitrification?

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